Modern agricultural treatment affects soils by reducing their microbial flora and stimulates pathogens to resist the effect of agrochemicals, although there are biological options on the market, these are not competitive, different efforts are made to develop biological controllers that increase the percentage of viable spores, better performance of the substrate design and aggressive antagonism against the pathogen, to carry out these evaluations the present research applied a random factorial design resulting in a matrix of 20 experimental units to determine the concentration values for the design of the substrate and optimal viability spores of Trichoderma spp. by solid fermentation applying a byproduct of Oryza sativa (rice) as substrate.The study strains were molecularly identified as Trichoderma Harzianum sp. and Fusarium oxisporum sp. which were faced in antagonism tests identifyi...