The Junín sheep is a rustic Peruvian breed adapted to the high Andean areas of the central highlands. It began with a population base of Creole sheep crossed with Corriedale sheep and with subsequent crosses using males from the North American west (Columbia, Panama and Warhill) as well as Corriedale rams from the USA and New Zealand. The livestock company SAIS Tupac Amaru Ltda. N.° 1 is the main depository of a population of around 100 000 animals. The present study aimed to evaluate the genetic diversity and population structure of this breed, as well as its relationship with other breeds. In total, 20 samples of sheep from the Junín herd were analyzed and high-density microarrays of single nucleotide polymorphic markers (SNPs) containing more than 500 000 SNP-type markers were used. The expected heterozygosity (HE) value was 0.429 and the inbreeding coefficient was 0.02. Regarding ...

The present study aimed to evaluate the immunogenic activity of three life attenuated strains of Salmonella Typhimurium isolated from guinea pig, by evaluating the excretion of faecal IgA in mice. The study was carried out with 28 four-week-old female mice of the Balb/c strain, distributed into four groups (seven mice per group): strain 1, strain 2, strain 3 and control group (PBS). Each mouse was inoculated with 109 CFU/100 µl of the attenuated strains using an orogastric probe. Fresh stool samples were collected at days 1, 9, 15 and 21 post-immunizations. The quantification of faecal IgA was carried out by an indirect in-house ELISA test. The results indicate that the mice inoculated with strains 1 and 2 presented a higher immunogenic activity (faecal IgA) compared to the Control group.