The purpose of this research was to study the effect of mass and humidity of eucalyptus leaves on the oil extraction performance of Eucalyptus Globulus by steam distillation. A unifactorial design was used, using different masses of eucalyptus leaves fed to the extraction chamber while maintaining operating conditions. An oil with a density of 0,906 g/ml has been obtained, refractive index of 1,462 and absolute viscosity of 2,28 cp. A maximum yield of 1,28% m/m was obtained with a humidity of 60,95%. It was determined that as the humidity of the leaves for oil extraction decreases, a higher yield is obtained. The optimal conditions for extraction were the following: cooling water flow: 102,5 L/h; steam flow rate for extraction: 2,67 L/h; extraction chamber volume: 0,016 m3; which resulted in an oil volume of 56,32 mL for a mass of 4 kg of leaves. The volume of oil extracted depends direc...