Mycoplasma haemofelis is the most pathogenic haemotropic species that affects felines by parasitizing red blood cells and inducing a haemolytic process that can lead to anaemia. The present study aimed to determine the prevalence of this infection in domestic felines from the city of Pereira, Risaralda, Colombia. Samples from 104 domestic felines between 1 and 10 years of age were analysed. Blood samples were taken for cytological (haematological parameters) and molecular analysis by PCR. Additionally, the possible risk factors associated with the presence of M. haemofelis (age, sex, vaccination status and geographical area of origin) were evaluated. The prevalence by molecular analysis was 80.8%, while it was 80.7% with the Giemsa stain and 81.7% with the Wright stain, and the youngest animals (1-2 years) being the most affected. A significant statistical correlation (p=0.0038) of age w...

The study aimed to determine the presence of the infectious agents that cause toxoplasmosis, brucellosis and leptospirosis using the conventional PCR molecular technique in domestic and wild cats, as well as in the personnel related to the care and management of these animals in the city of Pereira, Risaralda. (Colombia). Blood samples were taken from domestic cats (n=99) and wild cats in captivity (n=4) and from the personnel responsible for their care and management (veterinarians, zootechnicians, and operators; n=65). A prevalence of 37% for toxoplasmosis and 1% for leptospirosis in domestic felines was estimated. The presence of these pathogens was not found in the personnel.