The present investigation was aimed at obtaining the Median Lethal Dose and lethal time dose with five and seven time in nematodes (larvae of Diatraea sacharalis III Fabricius) using a Probit analysis. Statistical Analysis was performed in this research is the analysis of variance (ANOVA) of a completely randomized design, which allowed us to analyze whether or not statistically significant, which we conclude that if there is a difference among the five doses (0 ml, 2 ml, 4 ml, 8 ml and 16 ml) of Heterorhabditis bacteriophora, finding that the dose which has a higher mortality rate is the dose of 8 ml and 16 ml, and no difference between both doses. It was found that the median lethal dose (DL50) is 0.6674 ml. We found that the mean lethal time (TL50) is 144.63 hours. Among the different methods that can be to find the DL50 and TL50, we can say that the most suitable is the Probit Analys...