This study evaluated alpaca oocytes that developed to metaphase II (MII), using different concentrations of follicle stimulating hormone (FSH) with and without equine chorionic gonadotropin (eCG) during in vitro maturation. Oocytes were obtained from ovaries of slaughtered alpacas. Oocytes were matured in vitro for 36h in TCM-99 and supplemented in groups with different doses of FSH: 0.5, 0.25, 0μg.mL-1 and eCG: 15, 5, 0IU.mL-1. Oocytes were stained with 2% lacmoid, and examined for their nuclear status. The parameter for comparisons between groups was the percentage of oocytes in MII. There was an interaction between FSH and eCG (P<0.05). A higher percentage of oocytes in MII were obtained when using 0.5μg.mL-1 of FSH with any concentration of 15, 5 or 0IU.mL-1 of eCG [58.4±1.94% (n=78); 59.5±1.94% (n=85); 54.3±0.56% (n=81); P<0.05]. A similar percentage was also found using 0.25μ...

This study evaluated alpaca oocytes that developed to metaphase II (MII), using different concentrations of follicle stimulating hormone (FSH) with and without equine chorionic gonadotropin (eCG) during in vitro maturation. Oocytes were obtained from ovaries of slaughtered alpacas. Oocytes were matured in vitro for 36h in TCM-99 and supplemented in groups with different doses of FSH: 0.5, 0.25, 0μg.mL-1 and eCG: 15, 5, 0IU.mL-1. Oocytes were stained with 2% lacmoid, and examined for their nuclear status. The parameter for comparisons between groups was the percentage of oocytes in MII. There was an interaction between FSH and eCG (P<0.05). A higher percentage of oocytes in MII were obtained when using 0.5μg.mL-1 of FSH with any concentration of 15, 5 or 0IU.mL-1 of eCG [58.4±1.94% (n=78); 59.5±1.94% (n=85); 54.3±0.56% (n=81); P<0.05]. A similar percentage was also found using 0.25μ...