Objective. To describe the barriers to access and availability in emergency clinical laboratories in two type IV hospitals with different geographical locations in the Zuliana Region. Method. Descriptive and comparative study, with a non-experimental cross-sectional design. Non-probabilistic sampling with a sample size of 290 service users, 80 from Hospital 1 (H1) and 210 from Hospital 2 (H2). The data were obtained through a mixed survey of own elaboration, validated by agreement between experts. Descriptive statistics and comparison of means were used, using the T Student technique at 95% confidence. Results. The main access barrier found was geographical, in H1 (97.5%) and H2 (71.9%). Differences were found in the form of transportation and time required to reach the health center; in H1 the patients used their vehicles (48.8%) with a transfer time of 13.25±4.71 minutes; and in H2 pu...