The objective of the work was focused on studying the biodiversity of ecotypes and aggressiveness ranges of Moniliophthora perniciosa, in Theobroma cacao. Plant material with the presence of the pathogen from 12 plantations of T. cacao L Nacional was sampled, analyzing its morphological characteristics, identification by PCR and the aggressiveness of the disease in national cacao EET-19. The basidiocarps differ in size and basidiospore production considering the strain MpLRBF-105 with the largest diameter of basidiocarp and basidiospore discharge. The basidiocarps differ in size and basidiospore production considering the strain MpLRBF-105 with the largest diameter of basidiocarp and basidiospore discharge. The content of lamelulas would be adding the increase of basidiospores in the strains (MpLRBF105 and MpBOLN107), with the largest dimensions between lengths of (11.58 to 12.01 µm) an...

El objetivo se enfocó en caracterizar el potencial antagonista de PGPR en inhibición al desarrollo micelial, conidios en F. oxysporum y esporas en M. roreri. La técnica de PCR se empleó para la identificación de F. oxysporum y M. roreri. Seleccionando las bacterias: Acinetobacter calcoaceticus BMR2-12, Klebsiella variicola BO3-4, Enterobacter asburiae BA4-19, Enterobacter asburiae PM3-14, Pseudomonas protegens CHA0, Pseudomonas veronii R4 y Bacillus subtilis. Se evaluaron actividades antagónicas: a) Inhibición micelial en F. oxysporum y M. roreri; b) Reducción en producción de conidios y esporas por 12 y 15 días post-incubación. La PCR confirmó la amplificación de 670 pb para F. oxysporum. La secuenciación de 750 pb definió un grado de similitud del 99% a M. roreri, al compararse con el GenBank de NCBI. La aplicación de B. subtilis es de mayor actividad antagónica a inhi...

El objetivo se enfocó en caracterizar el potencial antagonista de PGPR en inhibición al desarrollo micelial, conidios en F. oxysporum y esporas en M. roreri. La técnica de PCR se empleó para la identificación de F. oxysporum y M. roreri. Seleccionando las bacterias: Acinetobacter calcoaceticus BMR2-12, Klebsiella variicola BO3-4, Enterobacter asburiae BA4-19, Enterobacter asburiae PM3-14, Pseudomonas protegens CHA0, Pseudomonas veronii R4 y Bacillus subtilis. Se evaluaron actividades antagónicas: a) Inhibición micelial en F. oxysporum y M. roreri; b) Reducción en producción de conidios y esporas por 12 y 15 días post-incubación. La PCR confirmó la amplificación de 670 pb para F. oxysporum. La secuenciación de 750 pb definió un grado de similitud del 99% a M. roreri, al compararse con el GenBank de NCBI. La aplicación de B. subtilis es de mayor actividad antagónica a inhi...

The objective of the work focused on characterizing the biodiversity of Phytophthora spp. and rhizobacteria with potential for biocontrol in black pod disease in CCN-51. Nine plant samples with symptoms of the disease were collected from cocoa-producing areas, evaluating their morphological characteristics and identification by PCR. Different morphotypes of colonies are observed: cottony, regular and starry edge characteristic for Phytophthora spp. Electrophoresis profiles reveal an amplicon of 159 bp for P. palmivora, demonstrating its distribution in the nine zones. The tests on mycelial inhibition in vitro to P. palmivora, determined high levels of antagonism of 78 and 60% by direct confrontation with the rhizobacteria. These results in bio-protection to CCN-51 by applications of P. veronii R4, A. calcoaceticus BMR2-12, S. marcescens PM3-8 and P. protegens CHA0 are effective in the co...

The objective of the work focused on characterizing the biodiversity of Phytophthora spp. and rhizobacteria with potential for biocontrol in black pod disease in CCN-51. Nine plant samples with symptoms of the disease were collected from cocoa-producing areas, evaluating their morphological characteristics and identification by PCR. Different morphotypes of colonies are observed: cottony, regular and starry edge characteristic for Phytophthora spp. Electrophoresis profiles reveal an amplicon of 159 bp for P. palmivora, demonstrating its distribution in the nine zones. The tests on mycelial inhibition in vitro to P. palmivora, determined high levels of antagonism of 78 and 60% by direct confrontation with the rhizobacteria. These results in bio-protection to CCN-51 by applications of P. veronii R4, A. calcoaceticus BMR2-12, S. marcescens PM3-8 and P. protegens CHA0 are effective in the co...