Ectoparasites of the genus Neobenedenia Yamaguti, 1963, are responsible for causing mortality within aquaculture systems. Given their pronounced morphological resemblance, the identification of Neobenedenia species based on phenotypic characteristics poses significant challenges. Thus, molecular techniques are used to complement discrimination between species with very limited morphological variation, such as Neobenedenia melleni (MacCallum, 1927) Yamaguti 1963 and N. girellae (Hargis, 1955) Yamaguti, 1963. Molecular markers can enhance the ability to describe biological diversity and understand the biogeography of these species.  The gulf of California has traditionally hindered the exchange of genes for various species with different life histories. In this study, Neobenedenia specimens were collected from wild and captive Seriola rivoliana Valenciennes, 1833, at four locations wi...