Many methods have been reported for the extraction of oils from microalgae, either for food, medicinal purposes and recently as feedstock to produce biofuels. For the production of biofuels such as biodiesel generally are used oilseeds which have shown to have many limitations due to the availability of land and water for cultivation, low crop yields and more so that seeds are used to food. For this reason in recent years have been studying the feasibility of the use of microalgae to produce biodiesel which can overcome the above limitations. Several authors have methodologies for extracting of different microalgae oil whose ranges of extraction are on the order of 11.2% to 22.9%.This work presents a modified (improved) oil extraction method from the microalgae Nannocloropsis oculata previously lyophilized using ultrasound treatment in a mix oforganic solvents cyclohexane-iso...