The objective was to determine the relationship between knowledge and hygienic food preparation in mothers with children under 5 years of age at the Fernando Lores Health Establishment, Iquitos 2023. The type of research was quantitative, the design used was non-experimental, correlational and transversal, the sample included 103 mothers. The techniques were interview, observation and home visit; The instruments were the knowledge questionnaire on hygienic food preparation with a validity of 98.2% and a reliability of 96.25%, and the hygienic food preparation checklist with a validity of 98.5% and a reliability of 96.25%. of 96.25%. of 96.5%. Results: Regarding knowledge about hygienic food preparation, it was found that 66.0% of mothers had low knowledge, 21.4% had regular knowledge, while only 12.6% had high knowledge. In hygienic food preparation, 32.0% had adequate hygienic preparati...