ABSTRACT Essential oils (EOs) from Citrus are the main by-product of Citrus-processing industries. In addition to food/beverage and cosmetic applications, citrus EOs could also potentially be used as an alternative to antibiotics in food-producing animals. A commercial citrus EO—Brazilian Orange Terpenes (BOT)—was fractionated by vacuum fractional distillation to separate BOT into various fractions: F1, F2, F3, and F4. Next, the chemical composition and biological activities of BOT and its fractions were characterized. Results showed the three first fractions had a high relative amount of limonene (≥10.86), even higher than the whole BOT. Conversely, F4 presented a larger relative amount of BOT’s minor compounds (carvone, cis-carveol, trans-carveol, cis-p-Mentha-2,8-dien-1-ol, and trans-p-Mentha-2,8-dien-1-ol) and a very low relative amount of limonene (0.08–0.13). Antibacteria...

ABSTRACT Post-weaning diarrhea (PWD) is an often disease affecting piglets. It is caused mainly by enterotoxigenic Escherichia coli (ETEC) colonization in pig gut. Antibiotics has been used to prevent, combat and control PWD and its negative impact on the productivity of pig breeding sector. Nonetheless, antibiotics due to their wide antibacterial spectrum also can reach beneficial gut bacteria, such as Lactobacillus. Lately, essential oils (EOs) have emerged as a potential alternative to using antibiotics in animal breeding because of their effect on bacterial growth. Commonly, citrus EOs are by-products of food industry and the availability of these EOs in the worldwide market is huge. Thus, six commercials citrus EOs were evaluated on ETEC strains, as model of pathogenic bacteria, and on Lactobacillus species, as models of beneficial bacteria. In overall, citrus EOs exhibited a select...

ABSTRACT Essential oils (EOs) have become a promising alternative to antibiotic use in animal breeding because of their biological properties. Citrus EOs, a by-product of citrus processing industries, could be a feasible alternative due to their vast production worldwide. Encapsulation techniques, such as microencapsulation, could enable EO application in animal feed, preserving EO bioactivity. This study analyzes the use of foodgrade polymers, chitosan and modified starch, for microencapsulation of a commercial citrus EO, Brazilian orange terpenes (BOT), by spray-drying. Physical properties characterization showed that the microencapsulated BOT (MB-OT) had a moisture content = 5.39 %, aw = 0.40 and bulk density = 0.42 g/mL. Overall, microparticles presented a slight irregular-spherical shape, with a mean diameter of 2.77 μm and high polydispersity. Encapsulation efficiency reached 61.1...

Essential oils (EOs) have turned a promising alternative to using antibiotics in poultry production due to their antimicrobial properties. EOs could effectively combat pathogenic bacteria affecting poultry. Particularly, Citrus EOs, a by-product of citrus processing industries, could be a feasible alternative to this end due to their vast availability in the global market. Enterococci are associated with intestinal and extra-intestinal infections in poultry, which can increase poultry mortality. On the other hand, Lactobacilli are beneficial bacteria inhabiting the poultry gut and have health-promoting effects. The aim of this study was to evaluate the antibacterial activity of a commercial citrus EO, Orange oil phase essence (OOPE), on Enterococcus faecalis and Lactobacillus rhamnosus as well as to determine OOPE chemical composition. Results showed that OOPE inhibited E. faecalis and L...

Food choice is often influenced by extrinsic factors, which drive consumer perception of a given product or service. It is therefore critical to study them during the design of food packaging. In this context, the aim of this study was to investigate the effect of extrinsic factors on the expected acceptance, purchase intention and holistic perception of consumers of the popular Peruvian dessert mazamorra morada. Twelve stimuli were created by using a fractional factorial design considering packaging material, nutritional information, quality, convenience, and naturalness as factors, previously obtained by open-ended questions. Ninety-seven consumers answered an online survey indicating their expected acceptance, purchase intention and holistic perception. The results showed that the most salient factors for consumers, in terms of expected acceptance, purchase intention, and holistic per...

Firstly, this study aimed to determine the effect of adding nisin (0, 250 and 500 IU mL-1) and oregano essential oil (OEO) (0, 0.025% and 0.05%), alone or in combination, on Staphylococcus aureus load in cow’s milk. Next, it was evaluated the sensory, physicochemical and microbiological quality of fresh cheese (FC) manufactured with milk added of nisin + OEO (C1) or nisin alone (C2) compared to a control cheese (C0, milk without the addition of nisin or OEO). Results showed that adding nisin effectively reduced the S. aureus count in milk in a similar manner to adding way the combination of nisin + OEO compared to the control. FC quality evaluation results revealed that C1 and C2 did not alter the physicochemical quality of FC (pH, fat and total solids content) during storage (0-8 days) compared to the control. Furthermore, C1 and C2 reduced in a similar manner aerobic mesophilic bacte...