Research correlational descriptive, cross-sectional, whose objective was to determine the level of knowledge and attitude of biosafety nursing internal Bethlehem Hospital of Trujillo. The universe consisted of 31 internal nursing who were applied two instruments "biosafety level of knowledge" and "attitude biosecurity". The processed results are presented and interpreted in tables of single and double entry. For statistical analysis the test criteria of independence (chi-square) was used reached the following conclusions: 80.6 percent of domestic nursing presented a regular level, good knowledge 12.9 percent, and 6.5 percent bad level. 58.1 percent of domestic nurses have a favorable attitude on Biosafety, 41.9 percent an unfavorable attitude on biosafety; no significant relationship exists between the level of knowledge and attitude of biosecurity. (p>0.05)