Aimed to know the effect of Pseudomonas aeruginosa bacteria, white fungus waste, and nano fertilizer on the pyrophosphatase enzyme, growth characteristics, and yield of stevia plants. The bacterial vaccine represented the first factor. It was added at two levels: B0 (without inoculum) and B1 (injecting 2 ml of liquid bio-inoculum represented by P. aeruginosa). The second factor was adding white fungus waste at three levels defined by Ab0 (without adding white mushroom waste), Ab1 (adding white mushroom waste at level 5 tons/h), and Ab2 (adding white mushroom waste at level 10 tons/h). Nano fertilizer was added as a third factor at four levels: N0 (without adding nano fertilizer), N1 (adding 4 kg/h of nano zinc), N2 (adding 2 kg/h of nano boron), and N3 (adding a mixture of 1 kg/h of nano boron + 2 kg/h of nano zinc). The triple combination B1Ab1N3 achieved a significant superiority in th...