One of the consequences of the misuse or abuse of antibiotics is that certain types of microorganisms become resistant to their effects. This makes it a public health problem. Therefore, the present research sought to identify the secondary metabolites and establish the antibacterial activity of the ethanolic extract of the leaves of Eschweilera juruensis R. Knuth (machimango) against Staphylococcus aureus ATCC 25923, Pseudomonas aeruginosa ATCC 27853 and Escherichia coli ATCC 25922 and their clinical isolates. Two methods were used: phytochemical screening, according to Schabra et al. and the Kirby-Bauer disk diffusion method, using concentrations of 125 mg/mL, 62.5 mg/mL and 31.25 mg/mL. Through phytochemical screening, the following secondary metabolites were identified: flavones, lactones, reducing sugars, alkaloids, triterpenes and steroids. Regarding the antibacterial activity, it ...