Two new species of Pavanelliella (Dactylogyridae) were described and some details about the morphology of Pavanelliella pavanellii Kritsky & Boeger, 1998 were added based on specimens collected from pimelodid fishes from Mogi Guaçu River, Brazil. Pavanelliella takemotoi sp. n., collected from nasal cavities of Pimelodus maculatus Lacepède, 1803 differs from its congeners by having a male copulatory organ (MCO) with 2–5 rings, vaginal canal sinuous without loops in distal portion of vaginal canal and anteriorly directed vagina containing five irregular rings around the vestibule. Pavanelliella laertei sp. n. collected from P. heraldoi Azpelicueta, 2001 is separated from the other species of the genus by lacking a vaginal vestibule and by distal end shape of accessory piece, which resemble flames or is bifurcated with one flank hooked in P. laertei sp. n. The specimens of P. pavane...