With the rise of nanotechnology, inorganic nanomaterials have been developed, which have a marked microbicide effect on a wide variety of microorganisms, such as viruses, bacteria and fungi. In the present work, the synthesis, characterization and study of the antimicrobial activity of copper oxide (I), cuprite, Cu2 O, obtained by chemical route was carried out. Copper sulfate pentahydrate was used as the precursor ascorbic acid and glucose, were used as organic reducers. The characterization was carried out by X-ray diffraction (XRD) and scanning electron microscopy (SEM). The in vitro antimicrobial activity of the cuprite particles on the Staphylococcus aureus bacterium was also determined, determining the Minimum Inhibitory Concentration (MIC). The results of the analysis by X-ray diffraction, confirmed that from the synthesis using ascorbic acid as a reducer and at pH = 1.66, 100% pu...