Comparative analysis of antimicrobial resistance in enterotoxigenic Escherichia coli isolates from two paediatric cohort studies in Lima, Peru

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El texto completo de este trabajo no está disponible en el Repositorio Académico UPC por restricciones de la casa editorial donde ha sido publicado.
Detalles Bibliográficos
Autores: Medina, Anicia M., Rivera, Fulton P., Pons, Maria J, Riveros, Maribel, Gomes, Cláudia, Bernal, María, Meza, Rina, Maves, Ryan C., Huicho, Luis, Chea Woo, Elsa, Lanata, Claudio F., Gil, Ana I., Ochoa, Theresa J., Ruiz, Joaquim
Formato: artículo
Fecha de Publicación:2015
Institución:Universidad Peruana de Ciencias Aplicadas
Repositorio:UPC-Institucional
Lenguaje:inglés
OAI Identifier:oai:repositorioacademico.upc.edu.pe:10757/609204
Enlace del recurso:http://hdl.handle.net/10757/609204
Nivel de acceso:acceso embargado
Materia:Antibiotic resistance
Children
Diarrhoea
Enterotoxigenic Escherichia coli
Peru
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network_acronym_str UUPC
network_name_str UPC-Institucional
repository_id_str 2670
dc.title.es_PE.fl_str_mv Comparative analysis of antimicrobial resistance in enterotoxigenic Escherichia coli isolates from two paediatric cohort studies in Lima, Peru
title Comparative analysis of antimicrobial resistance in enterotoxigenic Escherichia coli isolates from two paediatric cohort studies in Lima, Peru
spellingShingle Comparative analysis of antimicrobial resistance in enterotoxigenic Escherichia coli isolates from two paediatric cohort studies in Lima, Peru
Medina, Anicia M.
Antibiotic resistance
Children
Diarrhoea
Enterotoxigenic Escherichia coli
Peru
title_short Comparative analysis of antimicrobial resistance in enterotoxigenic Escherichia coli isolates from two paediatric cohort studies in Lima, Peru
title_full Comparative analysis of antimicrobial resistance in enterotoxigenic Escherichia coli isolates from two paediatric cohort studies in Lima, Peru
title_fullStr Comparative analysis of antimicrobial resistance in enterotoxigenic Escherichia coli isolates from two paediatric cohort studies in Lima, Peru
title_full_unstemmed Comparative analysis of antimicrobial resistance in enterotoxigenic Escherichia coli isolates from two paediatric cohort studies in Lima, Peru
title_sort Comparative analysis of antimicrobial resistance in enterotoxigenic Escherichia coli isolates from two paediatric cohort studies in Lima, Peru
author Medina, Anicia M.
author_facet Medina, Anicia M.
Rivera, Fulton P.
Pons, Maria J
Riveros, Maribel
Gomes, Cláudia
Bernal, María
Meza, Rina
Maves, Ryan C.
Huicho, Luis
Chea Woo, Elsa
Lanata, Claudio F.
Gil, Ana I.
Ochoa, Theresa J.
Ruiz, Joaquim
author_role author
author2 Rivera, Fulton P.
Pons, Maria J
Riveros, Maribel
Gomes, Cláudia
Bernal, María
Meza, Rina
Maves, Ryan C.
Huicho, Luis
Chea Woo, Elsa
Lanata, Claudio F.
Gil, Ana I.
Ochoa, Theresa J.
Ruiz, Joaquim
author2_role author
author
author
author
author
author
author
author
author
author
author
author
author
dc.contributor.email.es_PE.fl_str_mv joruiz@clinic.ub.es
dc.contributor.author.fl_str_mv Medina, Anicia M.
Rivera, Fulton P.
Pons, Maria J
Riveros, Maribel
Gomes, Cláudia
Bernal, María
Meza, Rina
Maves, Ryan C.
Huicho, Luis
Chea Woo, Elsa
Lanata, Claudio F.
Gil, Ana I.
Ochoa, Theresa J.
Ruiz, Joaquim
dc.subject.es_PE.fl_str_mv Antibiotic resistance
Children
Diarrhoea
Enterotoxigenic Escherichia coli
Peru
topic Antibiotic resistance
Children
Diarrhoea
Enterotoxigenic Escherichia coli
Peru
description El texto completo de este trabajo no está disponible en el Repositorio Académico UPC por restricciones de la casa editorial donde ha sido publicado.
publishDate 2015
dc.date.accessioned.es_PE.fl_str_mv 2016-05-12T15:10:45Z
dc.date.available.es_PE.fl_str_mv 2016-05-12T15:10:45Z
dc.date.issued.fl_str_mv 2015-08
dc.type.es_PE.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.citation.es_PE.fl_str_mv Comparative analysis of antimicrobial resistance in enterotoxigenic Escherichia coli isolates from two paediatric cohort studies in Lima, Peru 2015, 109 (8):493 Transactions of The Royal Society of Tropical Medicine and Hygiene
dc.identifier.issn.es_PE.fl_str_mv 0035-9203
1878-3503
dc.identifier.doi.es_PE.fl_str_mv 10.1093/trstmh/trv054
dc.identifier.uri.es_PE.fl_str_mv http://hdl.handle.net/10757/609204
dc.identifier.journal.es_PE.fl_str_mv Transactions of The Royal Society of Tropical Medicine and Hygiene (Trans R Soc Trop Med Hyg.)
identifier_str_mv Comparative analysis of antimicrobial resistance in enterotoxigenic Escherichia coli isolates from two paediatric cohort studies in Lima, Peru 2015, 109 (8):493 Transactions of The Royal Society of Tropical Medicine and Hygiene
0035-9203
1878-3503
10.1093/trstmh/trv054
Transactions of The Royal Society of Tropical Medicine and Hygiene (Trans R Soc Trop Med Hyg.)
url http://hdl.handle.net/10757/609204
dc.language.iso.es_PE.fl_str_mv eng
language eng
dc.relation.url.es_PE.fl_str_mv http://trstmh.oxfordjournals.org/lookup/doi/10.1093/trstmh/trv054
dc.rights.es_PE.fl_str_mv info:eu-repo/semantics/embargoedAccess
eu_rights_str_mv embargoedAccess
dc.format.es_PE.fl_str_mv application/pdf
dc.publisher.es_PE.fl_str_mv Oxford University Press
dc.source.es_PE.fl_str_mv Universidad Peruana de Ciencias Aplicadas (UPC)
Repositorio Académico - UPC
dc.source.none.fl_str_mv reponame:UPC-Institucional
instname:Universidad Peruana de Ciencias Aplicadas
instacron:UPC
instname_str Universidad Peruana de Ciencias Aplicadas
instacron_str UPC
institution UPC
reponame_str UPC-Institucional
collection UPC-Institucional
bitstream.url.fl_str_mv https://repositorioacademico.upc.edu.pe/bitstream/10757/609204/1/license.txt
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bitstream.checksumAlgorithm.fl_str_mv MD5
repository.name.fl_str_mv Repositorio académico upc
repository.mail.fl_str_mv upc@openrepository.com
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spelling Medina, Anicia M.Rivera, Fulton P.Pons, Maria JRiveros, MaribelGomes, CláudiaBernal, MaríaMeza, RinaMaves, Ryan C.Huicho, LuisChea Woo, ElsaLanata, Claudio F.Gil, Ana I.Ochoa, Theresa J.Ruiz, Joaquimjoruiz@clinic.ub.es2016-05-12T15:10:45Z2016-05-12T15:10:45Z2015-08Comparative analysis of antimicrobial resistance in enterotoxigenic Escherichia coli isolates from two paediatric cohort studies in Lima, Peru 2015, 109 (8):493 Transactions of The Royal Society of Tropical Medicine and Hygiene0035-92031878-350310.1093/trstmh/trv054http://hdl.handle.net/10757/609204Transactions of The Royal Society of Tropical Medicine and Hygiene (Trans R Soc Trop Med Hyg.)El texto completo de este trabajo no está disponible en el Repositorio Académico UPC por restricciones de la casa editorial donde ha sido publicado.Background Antibiotic resistance is increasing worldwide, being of special concern in low- and middle-income countries. The aim of this study was to determine the antimicrobial susceptibility and mechanisms of resistance in 205 enterotoxigenic Escherichia coli (ETEC) isolates from two cohort studies in children <24 months in Lima, Peru. Methods ETEC were identified by an in-house multiplex real-time PCR. Susceptibility to 13 antimicrobial agents was tested by disk diffusion; mechanisms of resistance were evaluated by PCR. Results ETEC isolates were resistant to ampicillin (64%), cotrimoxazole (52%), tetracycline (37%); 39% of the isolates were multidrug-resistant. Heat-stable toxin producing (ETEC-st) (48%) and heat-labile toxin producing ETEC (ETEC-lt) (40%) had higher rates of multidrug resistance than isolates producing both toxins (ETEC-lt-st) (21%), p<0.05. Only 10% of isolates were resistant to nalidixic acid and none to ciprofloxacin or cefotaxime. Ampicillin and sulfamethoxazole resistance were most often associated with blaTEM (69%) and sul2 genes (68%), respectively. Tetracycline resistance was associated with tet(A) (49%) and tet(B) (39%) genes. Azithromycin inhibitory diameters were ≤15 mm in 36% of isolates, with 5% of those presenting the mph(A) gene. Conclusions ETEC from Peruvian children are often resistant to older, inexpensive antibiotics, while remaining susceptible to ciprofloxacin, cephalosporins and furazolidone. Fluoroquinolones and azithromycin remain the drugs of choice for ETEC infections in Peru. However, further development of resistance should be closely monitored.Revisión por paresapplication/pdfengOxford University Presshttp://trstmh.oxfordjournals.org/lookup/doi/10.1093/trstmh/trv054info:eu-repo/semantics/embargoedAccessUniversidad Peruana de Ciencias Aplicadas (UPC)Repositorio Académico - UPCreponame:UPC-Institucionalinstname:Universidad Peruana de Ciencias Aplicadasinstacron:UPCAntibiotic resistance67e766ba-d831-44cf-bf46-b050ff171470600Children5ad1aad6-4658-4cfa-98fe-2e470fd1ab32600Diarrhoeae46a89b1-0af8-44b3-9da5-544671ba0e3b600Enterotoxigenic Escherichia colifdab1a5e-82c7-46d4-bd0c-7a6e8a52dbed600Perud6e19394-1ad0-4de6-8bb9-5ec7e2e6d476600Comparative analysis of antimicrobial resistance in enterotoxigenic Escherichia coli isolates from two paediatric cohort studies in Lima, Peruinfo:eu-repo/semantics/articleBackground Antibiotic resistance is increasing worldwide, being of special concern in low- and middle-income countries. The aim of this study was to determine the antimicrobial susceptibility and mechanisms of resistance in 205 enterotoxigenic Escherichia coli (ETEC) isolates from two cohort studies in children <24 months in Lima, Peru. Methods ETEC were identified by an in-house multiplex real-time PCR. Susceptibility to 13 antimicrobial agents was tested by disk diffusion; mechanisms of resistance were evaluated by PCR. Results ETEC isolates were resistant to ampicillin (64%), cotrimoxazole (52%), tetracycline (37%); 39% of the isolates were multidrug-resistant. Heat-stable toxin producing (ETEC-st) (48%) and heat-labile toxin producing ETEC (ETEC-lt) (40%) had higher rates of multidrug resistance than isolates producing both toxins (ETEC-lt-st) (21%), p<0.05. Only 10% of isolates were resistant to nalidixic acid and none to ciprofloxacin or cefotaxime. Ampicillin and sulfamethoxazole resistance were most often associated with blaTEM (69%) and sul2 genes (68%), respectively. Tetracycline resistance was associated with tet(A) (49%) and tet(B) (39%) genes. Azithromycin inhibitory diameters were ≤15 mm in 36% of isolates, with 5% of those presenting the mph(A) gene. Conclusions ETEC from Peruvian children are often resistant to older, inexpensive antibiotics, while remaining susceptible to ciprofloxacin, cephalosporins and furazolidone. Fluoroquinolones and azithromycin remain the drugs of choice for ETEC infections in Peru. However, further development of resistance should be closely monitored.LICENSElicense.txtlicense.txttext/plain; charset=utf-81659https://repositorioacademico.upc.edu.pe/bitstream/10757/609204/1/license.txt1ed8f33c5404431ad7aabc05080746c5MD51false10757/609204oai:repositorioacademico.upc.edu.pe:10757/6092042019-08-30 07:48:19.312Repositorio académico upcupc@openrepository.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Comparative analysis of antimicrobial resistance in enterotoxigenic Escherichia coli isolates from two paediatric cohort studies in Lima, Peru
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