Direct blood analysis of Bartonella bacilliformis Multi Locus Sequence Typing in patients with Oroya’s fever during a Peruvian outbreak

Descripción del Articulo

ASTMH 64th Annual Meeting. October 25-29, 2015 Philadelphia Marriott Downtown Philadelphia, Pennsylvania USA
Detalles Bibliográficos
Autores: Pons, Maria J., Silva, Wilmer, Gomes, Cláudia, Ruiz, Joaquim, Del Valle Mendoza, Juana Mercedes, Universidad Peruana de Ciencias Aplicadas (UPC)
Formato: objeto de conferencia
Fecha de Publicación:2015
Institución:Universidad Peruana de Ciencias Aplicadas
Repositorio:UPC-Institucional
Lenguaje:inglés
OAI Identifier:oai:repositorioacademico.upc.edu.pe:10757/582375
Enlace del recurso:http://hdl.handle.net/10757/582375
Nivel de acceso:acceso abierto
Materia:Bartonella bacilliformis
Peru
https://purl.org/pe-repo/ocde/ford#3.00.00
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dc.title.es_PE.fl_str_mv Direct blood analysis of Bartonella bacilliformis Multi Locus Sequence Typing in patients with Oroya’s fever during a Peruvian outbreak
title Direct blood analysis of Bartonella bacilliformis Multi Locus Sequence Typing in patients with Oroya’s fever during a Peruvian outbreak
spellingShingle Direct blood analysis of Bartonella bacilliformis Multi Locus Sequence Typing in patients with Oroya’s fever during a Peruvian outbreak
Pons, Maria J.
Bartonella bacilliformis
Peru
https://purl.org/pe-repo/ocde/ford#3.00.00
title_short Direct blood analysis of Bartonella bacilliformis Multi Locus Sequence Typing in patients with Oroya’s fever during a Peruvian outbreak
title_full Direct blood analysis of Bartonella bacilliformis Multi Locus Sequence Typing in patients with Oroya’s fever during a Peruvian outbreak
title_fullStr Direct blood analysis of Bartonella bacilliformis Multi Locus Sequence Typing in patients with Oroya’s fever during a Peruvian outbreak
title_full_unstemmed Direct blood analysis of Bartonella bacilliformis Multi Locus Sequence Typing in patients with Oroya’s fever during a Peruvian outbreak
title_sort Direct blood analysis of Bartonella bacilliformis Multi Locus Sequence Typing in patients with Oroya’s fever during a Peruvian outbreak
dc.creator.es_PE.fl_str_mv Universidad Peruana de Ciencias Aplicadas (UPC)
author Pons, Maria J.
author_facet Pons, Maria J.
Silva, Wilmer
Gomes, Cláudia
Ruiz, Joaquim
Del Valle Mendoza, Juana Mercedes
Universidad Peruana de Ciencias Aplicadas (UPC)
author_role author
author2 Silva, Wilmer
Gomes, Cláudia
Ruiz, Joaquim
Del Valle Mendoza, Juana Mercedes
Universidad Peruana de Ciencias Aplicadas (UPC)
author2_role author
author
author
author
author
dc.contributor.author.fl_str_mv Pons, Maria J.
Silva, Wilmer
Gomes, Cláudia
Ruiz, Joaquim
Del Valle Mendoza, Juana Mercedes
dc.subject.es_PE.fl_str_mv Bartonella bacilliformis
Peru
topic Bartonella bacilliformis
Peru
https://purl.org/pe-repo/ocde/ford#3.00.00
dc.subject.ocde.es_PE.fl_str_mv https://purl.org/pe-repo/ocde/ford#3.00.00
description ASTMH 64th Annual Meeting. October 25-29, 2015 Philadelphia Marriott Downtown Philadelphia, Pennsylvania USA
publishDate 2015
dc.date.accessioned.es_PE.fl_str_mv 2015-11-18T21:10:38Z
dc.date.available.es_PE.fl_str_mv 2015-11-18T21:10:38Z
dc.date.issued.fl_str_mv 2015-11-18
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dc.language.iso.es_PE.fl_str_mv eng
language eng
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dc.publisher.es_PE.fl_str_mv Universidad Peruana de Ciencias Aplicadas (UPC)
dc.source.es_PE.fl_str_mv Universidad Peruana de Ciencias Aplicadas (UPC)
Repositorio Académico - UPC
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spelling 02047e40e6be0f3cd89d981ee769eb49-19ea67379a0374af84e7e5083032cbcb9-1a7c43f3affe955e89fe095769303ce4a-1a9aff6ea13b87ce5161f68519cf5544d-107b0477e1d55a0c217b087d0df738c08-1Pons, Maria J.Silva, WilmerGomes, CláudiaRuiz, JoaquimDel Valle Mendoza, Juana MercedesUniversidad Peruana de Ciencias Aplicadas (UPC)2015-11-18T21:10:38Z2015-11-18T21:10:38Z2015-11-18http://hdl.handle.net/10757/582375ASTMH 64th Annual Meeting. October 25-29, 2015 Philadelphia Marriott Downtown Philadelphia, Pennsylvania USAThe bacteria Bartonella bacilliformis is the etiological agent of Carrion’s disease, which is a neglected poverty-related disease, affecting Mountain Andean valleys of Peru, Colombia and Ecuador. This disease, in absence of treatment presents a high mortality during the acute phase, called Oroya’s Fever. The second phase is characterized by the development of dermal eruptions, known as “Peruvian wart”. This bacterium is a fastidious slow growing microorganism, being difficult and cumbersome to culture and isolate from clinical sources. Then, the available data about phylogenetic relationship in clinical samples are really scarce, but suggesting high variability. The aim of the study was to perform direct blood analysis of B. bacilliformis Multi Locus Sequence Typing (MLST), a genotyping tool, in patients with Oroya fever during an outbreak. The present study demonstrate that the direct blood MLST PCR is a technique useful in the phylogenic characterization of this fastidious microorganism endemic from Andean regions. In this study, we demonstrate that the outbreak of Oroya’s fever was caused by closely related Sequence Typing (ST) microorganisms and, additionally, new STs have been described.application/pdfengUniversidad Peruana de Ciencias Aplicadas (UPC)info:eu-repo/semantics/openAccessUniversidad Peruana de Ciencias Aplicadas (UPC)Repositorio Académico - UPCreponame:UPC-Institucionalinstname:Universidad Peruana de Ciencias Aplicadasinstacron:UPCBartonella bacilliformis1a74396f-0c70-46ea-8894-e3c1ca9a9d51600Perud6e19394-1ad0-4de6-8bb9-5ec7e2e6d476600https://purl.org/pe-repo/ocde/ford#3.00.00Direct blood analysis of Bartonella bacilliformis Multi Locus Sequence Typing in patients with Oroya’s fever during a Peruvian outbreakinfo:eu-repo/semantics/conferenceObject2018-06-16T16:58:12ZThe bacteria Bartonella bacilliformis is the etiological agent of Carrion’s disease, which is a neglected poverty-related disease, affecting Mountain Andean valleys of Peru, Colombia and Ecuador. This disease, in absence of treatment presents a high mortality during the acute phase, called Oroya’s Fever. The second phase is characterized by the development of dermal eruptions, known as “Peruvian wart”. This bacterium is a fastidious slow growing microorganism, being difficult and cumbersome to culture and isolate from clinical sources. Then, the available data about phylogenetic relationship in clinical samples are really scarce, but suggesting high variability. The aim of the study was to perform direct blood analysis of B. bacilliformis Multi Locus Sequence Typing (MLST), a genotyping tool, in patients with Oroya fever during an outbreak. The present study demonstrate that the direct blood MLST PCR is a technique useful in the phylogenic characterization of this fastidious microorganism endemic from Andean regions. 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Direct blood analysis of Bartonella bacilliformis Multi Locus Sequence Typing in patients with Oroya’s fever during a Peruvian outbreak
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