Aggregatibacter actinomycetemcomitans in periodontal disease, identification through the PCR technique

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Objectives: The aim of this article was to investigate the pathogen Aggregatibacter actinomycetemcomitans (A. actinomycetemcomitans) and identify it by polymerase chain reaction (PCR). Materials and methods: The alkaline lysis technique was used for DNA extraction, and a segment of the 16S rRNA gene...

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Autores: Orellana Bravo , Paola Patricia, Andrade Tacuri, Carlos Fernando, Masabanda Ibarra, Stalin Mauricio, Centeno Dávila , María del Cisne, Tenemaza Tiban, Javier Marcelo, Mogrovejo Suarez , John Xavier, Montenegro Delgado, Carlos Joaquin, Ledesma Villacis, Nathaly Fernanda
Formato: artículo
Fecha de Publicación:2024
Institución:Universidad de San Martín de Porres
Repositorio:Revistas - Universidad de San Martín de Porres
Lenguaje:español
OAI Identifier:oai:revistas.usmp.edu.pe:article/2988
Enlace del recurso:https://portalrevistas.aulavirtualusmp.pe/index.php/Rev-Kiru0/article/view/2988
Nivel de acceso:acceso abierto
Materia:Aggregatibacter actinomycetemcomitans; Periodontal Diseases; Polymerase Chain Reaction
Aggregatibacter actinomycetemcomitans; Enfermedades periodontales; Reacción en Cadena de la Polimerasa
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spelling Aggregatibacter actinomycetemcomitans in periodontal disease, identification through the PCR techniqueAggregatibacter actinomycetemcomitans en enfermedad periodontal, identificación mediante la técnica de PCR Orellana Bravo , Paola Patricia Andrade Tacuri, Carlos Fernando Masabanda Ibarra, Stalin Mauricio Centeno Dávila , María del Cisne Tenemaza Tiban, Javier Marcelo Mogrovejo Suarez , John Xavier Montenegro Delgado, Carlos Joaquin Ledesma Villacis, Nathaly Fernanda Aggregatibacter actinomycetemcomitans; Periodontal Diseases; Polymerase Chain Reaction Aggregatibacter actinomycetemcomitans; Enfermedades periodontales; Reacción en Cadena de la PolimerasaObjectives: The aim of this article was to investigate the pathogen Aggregatibacter actinomycetemcomitans (A. actinomycetemcomitans) and identify it by polymerase chain reaction (PCR). Materials and methods: The alkaline lysis technique was used for DNA extraction, and a segment of the 16S rRNA gene of A. actinomycetemcomitans was amplified by PCR and specific primers. The specificity of the primers was tested using genetic material extracted from the reference strain A. actinomycetemcomitans ATCC 33384, against other strains. Results: This pathogen was investigated in detail and A. actinomycetemcomitans was identified by means of specific primers. Conclusions: A sensitive and specific technique for the detection of A. actinomycetemcomitans was obtained by conventional PCR.Objetivos: La finalidad del presente artículo fue investigar sobre el patógeno Aggregatibacter actinomycetemcomitans (A. actinomycetemcomitans) e identificarlo mediante la reacción en cadena de la polimerasa (PCR). Materiales y métodos: Se utilizó la técnica de lisis alcalina para la extracción de ácido desoxirribonucleico (ADN), mediante PCR e iniciadores específicos se amplificó un segmento del gen ácido ribonucleico ribosómico (ARNr) 16S de A. actinomycetemcomitans. La especificidad de los iniciadores se ensayó utilizando material genético extraído de la cepa de referencia A. actinomycetemcomitans ATCC 33384, frente a otras cepas. Resultados: Se investigó a detalle este patógeno y se identificó mediante primers específicos a A. actinomycetemcomitans. Conclusiones: Mediante PCR convencional se obtuvo una técnica sensible y específica para la detección de A. actinomycetemcomitans.  Universidad de San Martín de Porres, Facultad de Odontología.2024-10-31info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdftext/xmlhttps://portalrevistas.aulavirtualusmp.pe/index.php/Rev-Kiru0/article/view/298810.24265/kiru.2024.v21n4.04KIRU ; Vol. 21 No. 4 (2024): KIRU (OCTUBRE - DICIEMBRE); 205-210 KIRU ISSN electrónico 2410-2717 ISSN Impreso 1812 - 7886; Vol. 21 Núm. 4 (2024): KIRU (OCTUBRE - DICIEMBRE); 205-2102410-27171812-7886reponame:Revistas - Universidad de San Martín de Porresinstname:Universidad de San Martín de Porresinstacron:USMPspahttps://portalrevistas.aulavirtualusmp.pe/index.php/Rev-Kiru0/article/view/2988/3734https://portalrevistas.aulavirtualusmp.pe/index.php/Rev-Kiru0/article/view/2988/3735Derechos de autor 2024 Paola Patricia Orellana Bravo , Carlos Fernando Andrade Tacuri, Stalin Mauricio Masabanda Ibarra, María del Cisne Centeno Dávila , Javier Marcelo Tenemaza Tiban, John Xavier Mogrovejo Suarez , Carlos Joaquin Montenegro Delgado, Nathaly Fernanda Ledesma Villacishttps://creativecommons.org/licenses/by/4.0info:eu-repo/semantics/openAccessoai:revistas.usmp.edu.pe:article/29882025-06-17T19:14:43Z
dc.title.none.fl_str_mv Aggregatibacter actinomycetemcomitans in periodontal disease, identification through the PCR technique
Aggregatibacter actinomycetemcomitans en enfermedad periodontal, identificación mediante la técnica de PCR
title Aggregatibacter actinomycetemcomitans in periodontal disease, identification through the PCR technique
spellingShingle Aggregatibacter actinomycetemcomitans in periodontal disease, identification through the PCR technique
Orellana Bravo , Paola Patricia
Aggregatibacter actinomycetemcomitans; Periodontal Diseases; Polymerase Chain Reaction
Aggregatibacter actinomycetemcomitans; Enfermedades periodontales; Reacción en Cadena de la Polimerasa
title_short Aggregatibacter actinomycetemcomitans in periodontal disease, identification through the PCR technique
title_full Aggregatibacter actinomycetemcomitans in periodontal disease, identification through the PCR technique
title_fullStr Aggregatibacter actinomycetemcomitans in periodontal disease, identification through the PCR technique
title_full_unstemmed Aggregatibacter actinomycetemcomitans in periodontal disease, identification through the PCR technique
title_sort Aggregatibacter actinomycetemcomitans in periodontal disease, identification through the PCR technique
dc.creator.none.fl_str_mv Orellana Bravo , Paola Patricia
Andrade Tacuri, Carlos Fernando
Masabanda Ibarra, Stalin Mauricio
Centeno Dávila , María del Cisne
Tenemaza Tiban, Javier Marcelo
Mogrovejo Suarez , John Xavier
Montenegro Delgado, Carlos Joaquin
Ledesma Villacis, Nathaly Fernanda
author Orellana Bravo , Paola Patricia
author_facet Orellana Bravo , Paola Patricia
Andrade Tacuri, Carlos Fernando
Masabanda Ibarra, Stalin Mauricio
Centeno Dávila , María del Cisne
Tenemaza Tiban, Javier Marcelo
Mogrovejo Suarez , John Xavier
Montenegro Delgado, Carlos Joaquin
Ledesma Villacis, Nathaly Fernanda
author_role author
author2 Andrade Tacuri, Carlos Fernando
Masabanda Ibarra, Stalin Mauricio
Centeno Dávila , María del Cisne
Tenemaza Tiban, Javier Marcelo
Mogrovejo Suarez , John Xavier
Montenegro Delgado, Carlos Joaquin
Ledesma Villacis, Nathaly Fernanda
author2_role author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Aggregatibacter actinomycetemcomitans; Periodontal Diseases; Polymerase Chain Reaction
Aggregatibacter actinomycetemcomitans; Enfermedades periodontales; Reacción en Cadena de la Polimerasa
topic Aggregatibacter actinomycetemcomitans; Periodontal Diseases; Polymerase Chain Reaction
Aggregatibacter actinomycetemcomitans; Enfermedades periodontales; Reacción en Cadena de la Polimerasa
description Objectives: The aim of this article was to investigate the pathogen Aggregatibacter actinomycetemcomitans (A. actinomycetemcomitans) and identify it by polymerase chain reaction (PCR). Materials and methods: The alkaline lysis technique was used for DNA extraction, and a segment of the 16S rRNA gene of A. actinomycetemcomitans was amplified by PCR and specific primers. The specificity of the primers was tested using genetic material extracted from the reference strain A. actinomycetemcomitans ATCC 33384, against other strains. Results: This pathogen was investigated in detail and A. actinomycetemcomitans was identified by means of specific primers. Conclusions: A sensitive and specific technique for the detection of A. actinomycetemcomitans was obtained by conventional PCR.
publishDate 2024
dc.date.none.fl_str_mv 2024-10-31
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://portalrevistas.aulavirtualusmp.pe/index.php/Rev-Kiru0/article/view/2988
10.24265/kiru.2024.v21n4.04
url https://portalrevistas.aulavirtualusmp.pe/index.php/Rev-Kiru0/article/view/2988
identifier_str_mv 10.24265/kiru.2024.v21n4.04
dc.language.none.fl_str_mv spa
language spa
dc.relation.none.fl_str_mv https://portalrevistas.aulavirtualusmp.pe/index.php/Rev-Kiru0/article/view/2988/3734
https://portalrevistas.aulavirtualusmp.pe/index.php/Rev-Kiru0/article/view/2988/3735
dc.rights.none.fl_str_mv https://creativecommons.org/licenses/by/4.0
info:eu-repo/semantics/openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/4.0
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
text/xml
dc.publisher.none.fl_str_mv Universidad de San Martín de Porres, Facultad de Odontología.
publisher.none.fl_str_mv Universidad de San Martín de Porres, Facultad de Odontología.
dc.source.none.fl_str_mv KIRU ; Vol. 21 No. 4 (2024): KIRU (OCTUBRE - DICIEMBRE); 205-210
KIRU ISSN electrónico 2410-2717 ISSN Impreso 1812 - 7886; Vol. 21 Núm. 4 (2024): KIRU (OCTUBRE - DICIEMBRE); 205-210
2410-2717
1812-7886
reponame:Revistas - Universidad de San Martín de Porres
instname:Universidad de San Martín de Porres
instacron:USMP
instname_str Universidad de San Martín de Porres
instacron_str USMP
institution USMP
reponame_str Revistas - Universidad de San Martín de Porres
collection Revistas - Universidad de San Martín de Porres
repository.name.fl_str_mv
repository.mail.fl_str_mv
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Aggregatibacter actinomycetemcomitans in periodontal disease, identification through the PCR technique
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