Inhibitory effect of the methanolic fraction of Oxapampa propolis on different isolated genotypes of Streptococcus mutans in children with caries
Descripción del Articulo
Objective: To determine the inhibitory effect of the methanolic fraction of propolis from Oxapampa, Peru, on different genotypes of Streptococcus mutans in children diagnosed with caries. Materials and methods: 150 oral swab samples were collected, seeded for isolation of S. mutans and then identifi...
| Autores: | , , , , , |
|---|---|
| Formato: | artículo |
| Fecha de Publicación: | 2024 |
| Institución: | Universidad Peruana Cayetano Heredia |
| Repositorio: | Revistas - Universidad Peruana Cayetano Heredia |
| Lenguaje: | español |
| OAI Identifier: | oai:revistas.upch.edu.pe:article/6158 |
| Enlace del recurso: | https://revistas.upch.edu.pe/index.php/REH/article/view/6158 |
| Nivel de acceso: | acceso abierto |
| Materia: | Streptococcus mutans extracto propóleo caries dental inhibición bacteriana propolis extract dental caries bacterial inhibition extrato de própolis cárie dentária inibição bacteriana |
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Revistas - Universidad Peruana Cayetano Heredia |
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Inhibitory effect of the methanolic fraction of Oxapampa propolis on different isolated genotypes of Streptococcus mutans in children with caries Efecto inhibitorio de la fracción metanólica del propóleo de Oxapampa en diferentes genotipos aislados de Streptococcus mutans en niños con caries Efeito inibitório da fração metanólica da própolis de Oxapampa em diferentes genótipos de Streptococcus mutans isolados de crianças com cárie |
| title |
Inhibitory effect of the methanolic fraction of Oxapampa propolis on different isolated genotypes of Streptococcus mutans in children with caries |
| spellingShingle |
Inhibitory effect of the methanolic fraction of Oxapampa propolis on different isolated genotypes of Streptococcus mutans in children with caries Vargas Gil, Mayra Lizeth Streptococcus mutans extracto propóleo caries dental inhibición bacteriana Streptococcus mutans propolis extract dental caries bacterial inhibition Streptococcus mutans extrato de própolis cárie dentária inibição bacteriana |
| title_short |
Inhibitory effect of the methanolic fraction of Oxapampa propolis on different isolated genotypes of Streptococcus mutans in children with caries |
| title_full |
Inhibitory effect of the methanolic fraction of Oxapampa propolis on different isolated genotypes of Streptococcus mutans in children with caries |
| title_fullStr |
Inhibitory effect of the methanolic fraction of Oxapampa propolis on different isolated genotypes of Streptococcus mutans in children with caries |
| title_full_unstemmed |
Inhibitory effect of the methanolic fraction of Oxapampa propolis on different isolated genotypes of Streptococcus mutans in children with caries |
| title_sort |
Inhibitory effect of the methanolic fraction of Oxapampa propolis on different isolated genotypes of Streptococcus mutans in children with caries |
| dc.creator.none.fl_str_mv |
Vargas Gil, Mayra Lizeth Millones Gómez, Pablo Alejandro Tay Chu Jon , Lidia Yileng Valderrama Negrón, Ana Cecilia Collantes Diaz, Ingrit Elida Maurtua Torres, Dora Jesús |
| author |
Vargas Gil, Mayra Lizeth |
| author_facet |
Vargas Gil, Mayra Lizeth Millones Gómez, Pablo Alejandro Tay Chu Jon , Lidia Yileng Valderrama Negrón, Ana Cecilia Collantes Diaz, Ingrit Elida Maurtua Torres, Dora Jesús |
| author_role |
author |
| author2 |
Millones Gómez, Pablo Alejandro Tay Chu Jon , Lidia Yileng Valderrama Negrón, Ana Cecilia Collantes Diaz, Ingrit Elida Maurtua Torres, Dora Jesús |
| author2_role |
author author author author author |
| dc.subject.none.fl_str_mv |
Streptococcus mutans extracto propóleo caries dental inhibición bacteriana Streptococcus mutans propolis extract dental caries bacterial inhibition Streptococcus mutans extrato de própolis cárie dentária inibição bacteriana |
| topic |
Streptococcus mutans extracto propóleo caries dental inhibición bacteriana Streptococcus mutans propolis extract dental caries bacterial inhibition Streptococcus mutans extrato de própolis cárie dentária inibição bacteriana |
| description |
Objective: To determine the inhibitory effect of the methanolic fraction of propolis from Oxapampa, Peru, on different genotypes of Streptococcus mutans in children diagnosed with caries. Materials and methods: 150 oral swab samples were collected, seeded for isolation of S. mutans and then identified by biochemical tests of carbohydrate fermentation, Api 20 Strep (bioMérieux) and conventional PCR. The 138 strains isolated and identified from patients and the S. mutans ATCC® 25175™ control were confronted with the methanolic fraction by the disk diffusion method, having as positive control 0.12% chlorhexidine and negative control Milli-Q water and DMSO (1:1). Results: It was found that the inhibition diameter of the strains extracted from children diagnosed with caries against the methanolic fraction showed greater diameter (14.13 mm) in relation to the strain of S. mutans ATCC® 25175™ (10.16 mm) with statistically significant differences. In the genotyping of the 138 strains with the different specific primers (c, e, f, and k), it was found that 63.77% belonged to genotype c, 21.73% to genotypes c and e, and 14.50% to genotypes c and f. Conclusions: The methanolic fraction of Oxapampa propolis presents inhibitory activity on S. mutans strains isolated and genotyped in children diagnosed with caries. In addition, it presents greater inhibitory activity in strains obtained from children compared to S. mutans ATCC® 25175™. |
| publishDate |
2024 |
| dc.date.none.fl_str_mv |
2024-12-23 |
| dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
| format |
article |
| status_str |
publishedVersion |
| dc.identifier.none.fl_str_mv |
https://revistas.upch.edu.pe/index.php/REH/article/view/6158 10.20453/reh.v34i4.6158 |
| url |
https://revistas.upch.edu.pe/index.php/REH/article/view/6158 |
| identifier_str_mv |
10.20453/reh.v34i4.6158 |
| dc.language.none.fl_str_mv |
spa |
| language |
spa |
| dc.relation.none.fl_str_mv |
https://revistas.upch.edu.pe/index.php/REH/article/view/6158/5912 https://revistas.upch.edu.pe/index.php/REH/article/view/6158/6096 |
| dc.rights.none.fl_str_mv |
http://creativecommons.org/licenses/by/4.0 info:eu-repo/semantics/openAccess |
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http://creativecommons.org/licenses/by/4.0 |
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openAccess |
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application/pdf text/xml |
| dc.publisher.none.fl_str_mv |
Universidad Peruana Cayetano Heredia |
| publisher.none.fl_str_mv |
Universidad Peruana Cayetano Heredia |
| dc.source.none.fl_str_mv |
Revista Estomatológica Herediana; Vol. 34 No. 4 (2024): Octubre-diciembre; 307-315 Revista Estomatológica Herediana; Vol. 34 Núm. 4 (2024): Octubre-diciembre; 307-315 Revista Estomatológica Herediana; v. 34 n. 4 (2024): Octubre-diciembre; 307-315 2225-7616 1019-4355 10.20453/reh.v34i4 reponame:Revistas - Universidad Peruana Cayetano Heredia instname:Universidad Peruana Cayetano Heredia instacron:UPCH |
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Universidad Peruana Cayetano Heredia |
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UPCH |
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UPCH |
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Revistas - Universidad Peruana Cayetano Heredia |
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Revistas - Universidad Peruana Cayetano Heredia |
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1842351109499256832 |
| spelling |
Inhibitory effect of the methanolic fraction of Oxapampa propolis on different isolated genotypes of Streptococcus mutans in children with cariesEfecto inhibitorio de la fracción metanólica del propóleo de Oxapampa en diferentes genotipos aislados de Streptococcus mutans en niños con cariesEfeito inibitório da fração metanólica da própolis de Oxapampa em diferentes genótipos de Streptococcus mutans isolados de crianças com cárieVargas Gil, Mayra LizethMillones Gómez, Pablo AlejandroTay Chu Jon , Lidia YilengValderrama Negrón, Ana CeciliaCollantes Diaz, Ingrit ElidaMaurtua Torres, Dora JesúsStreptococcus mutansextracto propóleo caries dentalinhibición bacterianaStreptococcus mutans propolis extractdental caries bacterial inhibitionStreptococcus mutans extrato de própoliscárie dentária inibição bacterianaObjective: To determine the inhibitory effect of the methanolic fraction of propolis from Oxapampa, Peru, on different genotypes of Streptococcus mutans in children diagnosed with caries. Materials and methods: 150 oral swab samples were collected, seeded for isolation of S. mutans and then identified by biochemical tests of carbohydrate fermentation, Api 20 Strep (bioMérieux) and conventional PCR. The 138 strains isolated and identified from patients and the S. mutans ATCC® 25175™ control were confronted with the methanolic fraction by the disk diffusion method, having as positive control 0.12% chlorhexidine and negative control Milli-Q water and DMSO (1:1). Results: It was found that the inhibition diameter of the strains extracted from children diagnosed with caries against the methanolic fraction showed greater diameter (14.13 mm) in relation to the strain of S. mutans ATCC® 25175™ (10.16 mm) with statistically significant differences. In the genotyping of the 138 strains with the different specific primers (c, e, f, and k), it was found that 63.77% belonged to genotype c, 21.73% to genotypes c and e, and 14.50% to genotypes c and f. Conclusions: The methanolic fraction of Oxapampa propolis presents inhibitory activity on S. mutans strains isolated and genotyped in children diagnosed with caries. In addition, it presents greater inhibitory activity in strains obtained from children compared to S. mutans ATCC® 25175™.RESUMEN Objetivo: Determinar el efecto inhibitorio de la fracción metanólica del propóleo de Oxapampa, Perú, en diferentes genotipos de Streptococcus mutans en niños diagnosticados con caries. Materiales y métodos: Se recolectaron 150 muestras de hisopado oral, las cuales fueron sembradas para el aislamiento de S. mutans y luego identificadas por pruebas bioquímicas de fermentación de carbohidratos, Api 20 Strep (bioMérieux) y PCR convencional. Las 138 cepas aisladas e identificadas de los pacientes y el control de S. mutans ATCC® 25175™ se enfrentaron a la fracción metanólica por el método de difusión en disco, teniendo como control positivo clorhexidina al 0,12 % y control negativo agua Milli-Q y DMSO (1:1). Resultados: Se encontró que el diámetro de inhibición de las cepas extraídas de los niños diagnosticados con caries frente a la fracción metanólica mostró mayor diámetro (14,13 mm) en relación con la cepa de S. mutans ATCC® 25175™ (10,16 mm) con diferencias estadísticamente significativas. En la genotipificación de las 138 cepas con los diferentes primers específicos (c, e, f y k), se encontró que el 63,77 % pertenecía al genotipo c, el 21,73 % a los genotipos c y e, y el 14,50 % a los genotipos c y f. Conclusiones: La fracción metanólica del propóleo de Oxapampa presenta actividad inhibitoria en cepas de S. mutans aisladas y genotipificadas en niños diagnosticados con caries. Además, presenta mayor actividad inhibitoria en las cepas obtenidas de los niños en comparación con el S. mutans ATCC® 25175™.Objetivo: Determinar o efeito inibitório da fração metanólica da própolis de Oxapampa, Peru, sobre diferentes genótipos de Streptococcus mutans em crianças com diagnóstico de cárie. Materiais e métodos: 150 amostras de swab oral foram coletadas, semeadas para isolamento de S. mutans e depois identificadas por testes bioquímicos de fermentação de carboidratos, Api 20 Strep (bioMérieux) e PCR convencional. As 138 cepas isoladas e identificadas dos pacientes e o controle S. mutans ATCC® 25175™ foram desafiados com a fração metanólica pelo método de difusão em disco, tendo clorexidina 0,12 % como controle positivo e água Milli-Q e DMSO (1:1) como controle negativo. Resultados: Verificou-se que o diâmetro de inibição das cepas extraídas de crianças diagnosticadas com cárie contra a fração metanólica apresentou maior diâmetro (14,13 mm) em relação à cepa S. mutans ATCC® 25175™ (10,16 mm) com diferenças estatisticamente significativas. Na genotipagem das 138 cepas com os diferentes primers específicos (c, e, f e k), verificou-se que 63,77 % pertenciam ao genótipo c, 21,73 % aos genótipos c e e, e 14,50 % aos genótipos c e f. Conclusões: A fração metanólica da própolis de Oxapampa apresenta atividade inibitória sobre cepas de S. mutans isoladas e genotipadas em crianças diagnosticadas com cárie. Além disso, apresenta maior atividade inibitória em cepas obtidas de crianças em comparação com S. mutans ATCC® 25175™.Universidad Peruana Cayetano Heredia2024-12-23info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdftext/xmlhttps://revistas.upch.edu.pe/index.php/REH/article/view/615810.20453/reh.v34i4.6158Revista Estomatológica Herediana; Vol. 34 No. 4 (2024): Octubre-diciembre; 307-315Revista Estomatológica Herediana; Vol. 34 Núm. 4 (2024): Octubre-diciembre; 307-315Revista Estomatológica Herediana; v. 34 n. 4 (2024): Octubre-diciembre; 307-3152225-76161019-435510.20453/reh.v34i4reponame:Revistas - Universidad Peruana Cayetano Herediainstname:Universidad Peruana Cayetano Herediainstacron:UPCHspahttps://revistas.upch.edu.pe/index.php/REH/article/view/6158/5912https://revistas.upch.edu.pe/index.php/REH/article/view/6158/6096Derechos de autor 2024 Mayra Lizeth Vargas Gil, Pablo Alejandro Millones Gómez, Lidia Yileng Tay Chu Jon , Ana Cecilia Valderrama Negrón, Ingrit Elida Collantes Diaz, Dora Jesús Maurtua Torreshttp://creativecommons.org/licenses/by/4.0info:eu-repo/semantics/openAccessoai:revistas.upch.edu.pe:article/61582025-04-21T17:29:44Z |
| score |
12.660138 |
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La información contenida en este registro es de entera responsabilidad de la institución que gestiona el repositorio institucional donde esta contenido este documento o set de datos. El CONCYTEC no se hace responsable por los contenidos (publicaciones y/o datos) accesibles a través del Repositorio Nacional Digital de Ciencia, Tecnología e Innovación de Acceso Abierto (ALICIA).
