Isolation and partial characterization of a myotoxin from Bothrops atrox snake venom (Ophidia: Viperidae)

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A myotoxin from Bothrops atrox snake venom was purified by cationic exchange on CM-Sephadex C-50 with 0,05M ammonium acetate pH 7. The myotoxin is a basic protein and by gel filtration and PAGE-SDS was demonstrated that protein has a molecular weight of 27 kDa and two polipeptides chain of 14 kDa ea...

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Detalles Bibliográficos
Autores: Huatuco, Sergio, Escobar, Enrique, Yarlequé, Armando
Formato: artículo
Fecha de Publicación:2004
Institución:Universidad Nacional Mayor de San Marcos
Repositorio:Revistas - Universidad Nacional Mayor de San Marcos
Lenguaje:español
OAI Identifier:oai:ojs.csi.unmsm:article/2436
Enlace del recurso:https://revistasinvestigacion.unmsm.edu.pe/index.php/rpb/article/view/2436
Nivel de acceso:acceso abierto
Materia:miotoxina
Bothrops atrox
veneno de serpiente
mionecrosis
myotoxin
snake venom
myonecrosis
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spelling Isolation and partial characterization of a myotoxin from Bothrops atrox snake venom (Ophidia: Viperidae)Aislamiento y caracterización parcial de una miotoxina del veneno de la serpiente Bothrops atrox (Ophidia: Viperidae)Huatuco, SergioEscobar, EnriqueYarlequé, ArmandomiotoxinaBothrops atroxveneno de serpientemionecrosismyotoxinBothrops atroxsnake venommyonecrosisA myotoxin from Bothrops atrox snake venom was purified by cationic exchange on CM-Sephadex C-50 with 0,05M ammonium acetate pH 7. The myotoxin is a basic protein and by gel filtration and PAGE-SDS was demonstrated that protein has a molecular weight of 27 kDa and two polipeptides chain of 14 kDa each one. The inoculation of myotoxin in gastrocnemius muscle of white mice produce liberation of creatin kinase as well as myonecrosis. The myotoxin has phospholipasic, anticoagulant and edematic activity, but not hemolytic activity.Se ha purificado una miotoxina del veneno de la serpiente Bothrops atrox, empleando una columna de intercambio catiónico de CM-Sephadex C-50 equilibrada con buffer acetato de amonio 0,05M a pH 7. La miotoxina es una proteína básica, y por cromatografía de filtración y PAGE-SDS se ha determinado que tiene un peso molecular de 27 kDa, estando formada por dos cadenas polipeptídicas de 14 kDa cada una. La inoculación de la miotoxina en el músculo gastrocnemius de ratones albinos produce la liberación de creatina kinasa así como la necrosis del tejido. La miotoxina tiene actividad de fosfolipasa, anticoagulante y edemática, pero no hemolítica.Universidad Nacional Mayor de San Marcos, Facultad de Ciencias Biológicas2004-07-15info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://revistasinvestigacion.unmsm.edu.pe/index.php/rpb/article/view/243610.15381/rpb.v11i1.2436Revista Peruana de Biología; Vol. 11 Núm. 1 (2004); 79-86Revista Peruana de Biología; Vol. 11 No. 1 (2004); 79-861727-99331561-0837reponame:Revistas - Universidad Nacional Mayor de San Marcosinstname:Universidad Nacional Mayor de San Marcosinstacron:UNMSMspahttps://revistasinvestigacion.unmsm.edu.pe/index.php/rpb/article/view/2436/2134Derechos de autor 2004 Sergio Huatuco, Enrique Escobar, Armando Yarlequéhttps://creativecommons.org/licenses/by-nc-sa/4.0info:eu-repo/semantics/openAccessoai:ojs.csi.unmsm:article/24362020-05-25T11:29:34Z
dc.title.none.fl_str_mv Isolation and partial characterization of a myotoxin from Bothrops atrox snake venom (Ophidia: Viperidae)
Aislamiento y caracterización parcial de una miotoxina del veneno de la serpiente Bothrops atrox (Ophidia: Viperidae)
title Isolation and partial characterization of a myotoxin from Bothrops atrox snake venom (Ophidia: Viperidae)
spellingShingle Isolation and partial characterization of a myotoxin from Bothrops atrox snake venom (Ophidia: Viperidae)
Huatuco, Sergio
miotoxina
Bothrops atrox
veneno de serpiente
mionecrosis
myotoxin
Bothrops atrox
snake venom
myonecrosis
title_short Isolation and partial characterization of a myotoxin from Bothrops atrox snake venom (Ophidia: Viperidae)
title_full Isolation and partial characterization of a myotoxin from Bothrops atrox snake venom (Ophidia: Viperidae)
title_fullStr Isolation and partial characterization of a myotoxin from Bothrops atrox snake venom (Ophidia: Viperidae)
title_full_unstemmed Isolation and partial characterization of a myotoxin from Bothrops atrox snake venom (Ophidia: Viperidae)
title_sort Isolation and partial characterization of a myotoxin from Bothrops atrox snake venom (Ophidia: Viperidae)
dc.creator.none.fl_str_mv Huatuco, Sergio
Escobar, Enrique
Yarlequé, Armando
author Huatuco, Sergio
author_facet Huatuco, Sergio
Escobar, Enrique
Yarlequé, Armando
author_role author
author2 Escobar, Enrique
Yarlequé, Armando
author2_role author
author
dc.subject.none.fl_str_mv miotoxina
Bothrops atrox
veneno de serpiente
mionecrosis
myotoxin
Bothrops atrox
snake venom
myonecrosis
topic miotoxina
Bothrops atrox
veneno de serpiente
mionecrosis
myotoxin
Bothrops atrox
snake venom
myonecrosis
description A myotoxin from Bothrops atrox snake venom was purified by cationic exchange on CM-Sephadex C-50 with 0,05M ammonium acetate pH 7. The myotoxin is a basic protein and by gel filtration and PAGE-SDS was demonstrated that protein has a molecular weight of 27 kDa and two polipeptides chain of 14 kDa each one. The inoculation of myotoxin in gastrocnemius muscle of white mice produce liberation of creatin kinase as well as myonecrosis. The myotoxin has phospholipasic, anticoagulant and edematic activity, but not hemolytic activity.
publishDate 2004
dc.date.none.fl_str_mv 2004-07-15
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://revistasinvestigacion.unmsm.edu.pe/index.php/rpb/article/view/2436
10.15381/rpb.v11i1.2436
url https://revistasinvestigacion.unmsm.edu.pe/index.php/rpb/article/view/2436
identifier_str_mv 10.15381/rpb.v11i1.2436
dc.language.none.fl_str_mv spa
language spa
dc.relation.none.fl_str_mv https://revistasinvestigacion.unmsm.edu.pe/index.php/rpb/article/view/2436/2134
dc.rights.none.fl_str_mv Derechos de autor 2004 Sergio Huatuco, Enrique Escobar, Armando Yarlequé
https://creativecommons.org/licenses/by-nc-sa/4.0
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Derechos de autor 2004 Sergio Huatuco, Enrique Escobar, Armando Yarlequé
https://creativecommons.org/licenses/by-nc-sa/4.0
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Universidad Nacional Mayor de San Marcos, Facultad de Ciencias Biológicas
publisher.none.fl_str_mv Universidad Nacional Mayor de San Marcos, Facultad de Ciencias Biológicas
dc.source.none.fl_str_mv Revista Peruana de Biología; Vol. 11 Núm. 1 (2004); 79-86
Revista Peruana de Biología; Vol. 11 No. 1 (2004); 79-86
1727-9933
1561-0837
reponame:Revistas - Universidad Nacional Mayor de San Marcos
instname:Universidad Nacional Mayor de San Marcos
instacron:UNMSM
instname_str Universidad Nacional Mayor de San Marcos
instacron_str UNMSM
institution UNMSM
reponame_str Revistas - Universidad Nacional Mayor de San Marcos
collection Revistas - Universidad Nacional Mayor de San Marcos
repository.name.fl_str_mv
repository.mail.fl_str_mv
_version_ 1795238309135384576
score 13.7211075
Isolation and partial characterization of a myotoxin from Bothrops atrox snake venom (Ophidia: Viperidae)
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