In vitro lethal activity of extract protein Bacillus thuringiensis against Nematodirus spathiger eggs and infective larvae (L3)

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A suspension containing the total protein extract of Bacillus thuringiensis of five strains (kurstaki HD-1, thompsoni HD-542, neoleonensis T-24-001, israeliensis HD-500 and israeliensis WHO-2013-9) was obtained. The concentration (mg/ml) of each strain was determined by the Biuret method. The nemati...

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Detalles Bibliográficos
Autores: Ochoa Silvera, Adolfo Alfredo, Mujica Lengua, Fidel Rodolfo
Formato: artículo
Fecha de Publicación:2015
Institución:Universidad Nacional Mayor de San Marcos
Repositorio:Revistas - Universidad Nacional Mayor de San Marcos
Lenguaje:español
OAI Identifier:oai:ojs.csi.unmsm:article/11187
Enlace del recurso:https://revistasinvestigacion.unmsm.edu.pe/index.php/veterinaria/article/view/11187
Nivel de acceso:acceso abierto
Materia:Bacillus thuringiensis
Nematodirus spathiger
biological control
control biológico
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spelling In vitro lethal activity of extract protein Bacillus thuringiensis against Nematodirus spathiger eggs and infective larvae (L3)Actividad Letal in vitro del Extracto Proteico Total de Bacillus thuringiensis sobre Huevos y Larvas (L3) Infectivas de Nematodirus SpathigerOchoa Silvera, Adolfo AlfredoMujica Lengua, Fidel RodolfoBacillus thuringiensisNematodirus spathigerbiological controlBacillus thuringiensisNematodirus spathigercontrol biológicoA suspension containing the total protein extract of Bacillus thuringiensis of five strains (kurstaki HD-1, thompsoni HD-542, neoleonensis T-24-001, israeliensis HD-500 and israeliensis WHO-2013-9) was obtained. The concentration (mg/ml) of each strain was determined by the Biuret method. The nematicidal activity was evaluated in Petri plates facing eggs and infective larvae (L3) of Nematodirus spathiger against the suspension of total protein extract (1.2 ml). Five treatments (strains) and 10 repetitions were used. The control group contained only the nematodes. The Petri dishes were incubated at 25 °C for 24, 48 and 72 h. Kurstaki HD-1 strain in a concentration of 5.042 mg/ml showed the best anthelmintic efficiency in the three incubation times, obtaining 44.7% mortality in eggs and 45.6% in larva. The total protein extract of B. thuringiensis kurstaki HD-1 contains the nematicidal toxins that could be used for future in vivo experimentally infected animals with N. spathiger.Se obtuvo una suspensión conteniendo el extracto proteico total de Bacillus thuringiensis de las variedades kurstaki HD-1, thompsoni HD-542, neoleonensis T-24- 001, israeliensis HD-500 e israeliensis WHO-2013-9 y se determinó la concentración (mg/ml) de cada variedad por el método de Biuret. La actividad nematicida se evaluó en placas de Petri enfrentando huevos y larvas (L3) infectivas de Nematodirus spathiger contra la suspensión del extracto proteico total (1.2 ml), considerando cinco tratamientos (variedades) y 10 repeticiones. El grupo control solo contenía nematodos. Las placas de Petri se incubaron a 25 °C por 24, 48 y 72 h. La variedad kurstaki HD-1 en una concentración de 5.042 mg/ml fue la variedad con mayor eficiencia antihelmíntica en los tres tiempos de incubación, obteniendo una mortalidad de 44.7% en huevos y 45.6% en larvas. El extracto proteico total de B. thuringiensis variedad kurstaki HD-1 contiene las toxinas nematicidas que pudieran ser empleadas para el futuro en pruebas in vivo con animales infectados experimentalmente con N. spathiger.Universidad Nacional Mayor de San Marcos, Facultad de Medicina Veterinaria2015-09-14info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://revistasinvestigacion.unmsm.edu.pe/index.php/veterinaria/article/view/1118710.15381/rivep.v26i3.11187Revista de Investigaciones Veterinarias del Perú; Vol. 26 Núm. 3 (2015); 509-518Revista de Investigaciones Veterinarias del Perú; Vol. 26 No. 3 (2015); 509-5181682-34191609-9117reponame:Revistas - Universidad Nacional Mayor de San Marcosinstname:Universidad Nacional Mayor de San Marcosinstacron:UNMSMspahttps://revistasinvestigacion.unmsm.edu.pe/index.php/veterinaria/article/view/11187/10273Derechos de autor 2015 Adolfo Alfredo Ochoa Silvera, Fidel Rodolfo Mujica Lenguahttps://creativecommons.org/licenses/by-nc-sa/4.0info:eu-repo/semantics/openAccessoai:ojs.csi.unmsm:article/111872020-03-29T19:24:08Z
dc.title.none.fl_str_mv In vitro lethal activity of extract protein Bacillus thuringiensis against Nematodirus spathiger eggs and infective larvae (L3)
Actividad Letal in vitro del Extracto Proteico Total de Bacillus thuringiensis sobre Huevos y Larvas (L3) Infectivas de Nematodirus Spathiger
title In vitro lethal activity of extract protein Bacillus thuringiensis against Nematodirus spathiger eggs and infective larvae (L3)
spellingShingle In vitro lethal activity of extract protein Bacillus thuringiensis against Nematodirus spathiger eggs and infective larvae (L3)
Ochoa Silvera, Adolfo Alfredo
Bacillus thuringiensis
Nematodirus spathiger
biological control
Bacillus thuringiensis
Nematodirus spathiger
control biológico
title_short In vitro lethal activity of extract protein Bacillus thuringiensis against Nematodirus spathiger eggs and infective larvae (L3)
title_full In vitro lethal activity of extract protein Bacillus thuringiensis against Nematodirus spathiger eggs and infective larvae (L3)
title_fullStr In vitro lethal activity of extract protein Bacillus thuringiensis against Nematodirus spathiger eggs and infective larvae (L3)
title_full_unstemmed In vitro lethal activity of extract protein Bacillus thuringiensis against Nematodirus spathiger eggs and infective larvae (L3)
title_sort In vitro lethal activity of extract protein Bacillus thuringiensis against Nematodirus spathiger eggs and infective larvae (L3)
dc.creator.none.fl_str_mv Ochoa Silvera, Adolfo Alfredo
Mujica Lengua, Fidel Rodolfo
author Ochoa Silvera, Adolfo Alfredo
author_facet Ochoa Silvera, Adolfo Alfredo
Mujica Lengua, Fidel Rodolfo
author_role author
author2 Mujica Lengua, Fidel Rodolfo
author2_role author
dc.subject.none.fl_str_mv Bacillus thuringiensis
Nematodirus spathiger
biological control
Bacillus thuringiensis
Nematodirus spathiger
control biológico
topic Bacillus thuringiensis
Nematodirus spathiger
biological control
Bacillus thuringiensis
Nematodirus spathiger
control biológico
description A suspension containing the total protein extract of Bacillus thuringiensis of five strains (kurstaki HD-1, thompsoni HD-542, neoleonensis T-24-001, israeliensis HD-500 and israeliensis WHO-2013-9) was obtained. The concentration (mg/ml) of each strain was determined by the Biuret method. The nematicidal activity was evaluated in Petri plates facing eggs and infective larvae (L3) of Nematodirus spathiger against the suspension of total protein extract (1.2 ml). Five treatments (strains) and 10 repetitions were used. The control group contained only the nematodes. The Petri dishes were incubated at 25 °C for 24, 48 and 72 h. Kurstaki HD-1 strain in a concentration of 5.042 mg/ml showed the best anthelmintic efficiency in the three incubation times, obtaining 44.7% mortality in eggs and 45.6% in larva. The total protein extract of B. thuringiensis kurstaki HD-1 contains the nematicidal toxins that could be used for future in vivo experimentally infected animals with N. spathiger.
publishDate 2015
dc.date.none.fl_str_mv 2015-09-14
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://revistasinvestigacion.unmsm.edu.pe/index.php/veterinaria/article/view/11187
10.15381/rivep.v26i3.11187
url https://revistasinvestigacion.unmsm.edu.pe/index.php/veterinaria/article/view/11187
identifier_str_mv 10.15381/rivep.v26i3.11187
dc.language.none.fl_str_mv spa
language spa
dc.relation.none.fl_str_mv https://revistasinvestigacion.unmsm.edu.pe/index.php/veterinaria/article/view/11187/10273
dc.rights.none.fl_str_mv Derechos de autor 2015 Adolfo Alfredo Ochoa Silvera, Fidel Rodolfo Mujica Lengua
https://creativecommons.org/licenses/by-nc-sa/4.0
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Derechos de autor 2015 Adolfo Alfredo Ochoa Silvera, Fidel Rodolfo Mujica Lengua
https://creativecommons.org/licenses/by-nc-sa/4.0
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Universidad Nacional Mayor de San Marcos, Facultad de Medicina Veterinaria
publisher.none.fl_str_mv Universidad Nacional Mayor de San Marcos, Facultad de Medicina Veterinaria
dc.source.none.fl_str_mv Revista de Investigaciones Veterinarias del Perú; Vol. 26 Núm. 3 (2015); 509-518
Revista de Investigaciones Veterinarias del Perú; Vol. 26 No. 3 (2015); 509-518
1682-3419
1609-9117
reponame:Revistas - Universidad Nacional Mayor de San Marcos
instname:Universidad Nacional Mayor de San Marcos
instacron:UNMSM
instname_str Universidad Nacional Mayor de San Marcos
instacron_str UNMSM
institution UNMSM
reponame_str Revistas - Universidad Nacional Mayor de San Marcos
collection Revistas - Universidad Nacional Mayor de San Marcos
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 13.971837
In vitro lethal activity of extract protein Bacillus thuringiensis against Nematodirus spathiger eggs and infective larvae (L3)
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