Identification of Malassezia species isolated from healthy skin in residents of Lima, Peru

• Autores: Bejar, Vilma, Rojas, Carlos, Guevara G., José M., Pareja, Elizabeth, Huamán, Ana, Sevilla, Raúl, Tapia, Mario, Zerpa, Rito, Gonzales, Sofía, Villanueva, Freddy, Valencia, Esther, Marocho, Luis, Abanto, Emma
• Formato: artículo
• Fecha de Publicación: 2014
• Institución: Universidad Nacional Mayor de San Marcos
• Repositorio: Revistas - Universidad Nacional Mayor de San Marcos
• Lenguaje: español
• OAI Identifier: oai:ojs.csi.unmsm:article/8347
• Enlace del recurso: https://revistasinvestigacion.unmsm.edu.pe/index.php/anales/article/view/8347
• Nivel de acceso: acceso abierto
• Materia: Malassezia spp; healthy skin; Tween assimilation; piel sana; asimilación de tween

Objective: To identify Malassezia species in healthy skin seborrhea areas in Lima inhabitants. Design: Cross-sectional study. Setting: Daniel Alcides Carrion Tropical Medicine Institute, Universidad Nacional Mayor de San Marcos, Lima, Peru. Participants: Asymptomatic persons. Interventions: Skin samples were collected from 129 asymptomatic residents of several districts of Lima city using Mariat and Adan-Campos’ technique. Malassezia isolation was performed in modified Dixon medium and incubated at 32° C for 7 days. Colonies were identified by macro and micro morphological characteristics and typing was determined by biochemical and physiological properties using Guillot’s technique. Main outcome measures: Malassezia species, participants’ gender, age and anatomical region. Results: Malassezia spp was isolated in 43.4% of the residents, 49.2% in men and 37.5% in women. From various body regions 68 cultures were positive: scalp 31 (45.6%), back 36 (52.9%) and frontal region 1 (1.5%). Isolates most common age group (47.2%) was that of adolescents-young (14-25 year-old). M. slooffiae was found in 83.8% and M. obtusa in 16.2% of cases. Conclusions: Malassezia spp. was present in healthy human skin. M. slooffiae was the predominant species in positive cases (83.8%) followed by M. obtusa (16.2%).