Fecal IgA levels in mice inoculated with attenuated strains of Salmonella Typhimurium
Descripción del Articulo
The present study aimed to evaluate the immunogenic activity of three life attenuated strains of Salmonella Typhimurium isolated from guinea pig, by evaluating the excretion of faecal IgA in mice. The study was carried out with 28 four-week-old female mice of the Balb/c strain, distributed into four...
| Autores: | , , , , |
|---|---|
| Formato: | artículo |
| Fecha de Publicación: | 2022 |
| Institución: | Universidad Nacional Mayor de San Marcos |
| Repositorio: | Revistas - Universidad Nacional Mayor de San Marcos |
| Lenguaje: | español |
| OAI Identifier: | oai:ojs.csi.unmsm:article/22898 |
| Enlace del recurso: | https://revistasinvestigacion.unmsm.edu.pe/index.php/veterinaria/article/view/22898 |
| Nivel de acceso: | acceso abierto |
| Materia: | attenuated strain Cavia porcellus immunogenicity immunoglobulin A Salmonella Typhimurium cepa atenuada inmunogenicidad inmunoglobulina A |
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Fecal IgA levels in mice inoculated with attenuated strains of Salmonella TyphimuriumNiveles de IgA fecal en ratones inoculados con cepas atenuadas de Salmonella TyphimuriumChanganaquí, ChristianLuna E., LuisCarhuaricra H., DennisMaturrano H., LeninRosadio A., RaúlChanganaquí, ChristianLuna E., LuisCarhuaricra H., DennisMaturrano H., LeninRosadio A., Raúlattenuated strainCavia porcellusimmunogenicityimmunoglobulin ASalmonella Typhimuriumcepa atenuadaCavia porcellusinmunogenicidadinmunoglobulina ASalmonella TyphimuriumThe present study aimed to evaluate the immunogenic activity of three life attenuated strains of Salmonella Typhimurium isolated from guinea pig, by evaluating the excretion of faecal IgA in mice. The study was carried out with 28 four-week-old female mice of the Balb/c strain, distributed into four groups (seven mice per group): strain 1, strain 2, strain 3 and control group (PBS). Each mouse was inoculated with 109 CFU/100 µl of the attenuated strains using an orogastric probe. Fresh stool samples were collected at days 1, 9, 15 and 21 post-immunizations. The quantification of faecal IgA was carried out by an indirect in-house ELISA test. The results indicate that the mice inoculated with strains 1 and 2 presented a higher immunogenic activity (faecal IgA) compared to the Control group.El presente estudio tuvo como objetivo evaluar la actividad inmunogénica de tres cepas vivas atenuadas de Salmonella Typhimurium aislados de cuy, mediante la evaluación de la excreción de IgA fecal en ratones. El estudio se realizó con 28 ratones hembra de la cepa Balb/c de cuatro semanas de edad, divididos en cuatro grupos (siete ratones por grupo): cepa 1, cepa 2, cepa 3 y grupo control (PBS). Cada ratón fue inoculado con 109 UFC/100 µl de las cepas atenuadas por vía oral mediante el uso de una sonda orogástrica. Se recolectaron muestras de heces frescas a los días 1, 9, 15 y 21 días pos-inmunización. La cuantificación de IgA fecal fue realizada mediante una prueba de ELISA in house indirecto. Los resultados indican que los ratones inoculados con las cepas 1 y 2 presentaron una mayor actividad inmunogénica (IgA fecal) frente al grupo control.Universidad Nacional Mayor de San Marcos, Facultad de Medicina Veterinaria2022-06-29info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://revistasinvestigacion.unmsm.edu.pe/index.php/veterinaria/article/view/2289810.15381/rivep.v33i3.22898Revista de Investigaciones Veterinarias del Perú; Vol. 33 Núm. 3 (2022); e22898Revista de Investigaciones Veterinarias del Perú; Vol. 33 No. 3 (2022); e228981682-34191609-9117reponame:Revistas - Universidad Nacional Mayor de San Marcosinstname:Universidad Nacional Mayor de San Marcosinstacron:UNMSMspahttps://revistasinvestigacion.unmsm.edu.pe/index.php/veterinaria/article/view/22898/18200Derechos de autor 2022 Christian Changanaquí, Luis Luna E., Dennis Carhuaricra H., Lenin Maturrano H., Raúl Rosadio A.http://creativecommons.org/licenses/by/4.0info:eu-repo/semantics/openAccessoai:ojs.csi.unmsm:article/228982022-06-30T13:14:24Z |
| dc.title.none.fl_str_mv |
Fecal IgA levels in mice inoculated with attenuated strains of Salmonella Typhimurium Niveles de IgA fecal en ratones inoculados con cepas atenuadas de Salmonella Typhimurium |
| title |
Fecal IgA levels in mice inoculated with attenuated strains of Salmonella Typhimurium |
| spellingShingle |
Fecal IgA levels in mice inoculated with attenuated strains of Salmonella Typhimurium Changanaquí, Christian attenuated strain Cavia porcellus immunogenicity immunoglobulin A Salmonella Typhimurium cepa atenuada Cavia porcellus inmunogenicidad inmunoglobulina A Salmonella Typhimurium |
| title_short |
Fecal IgA levels in mice inoculated with attenuated strains of Salmonella Typhimurium |
| title_full |
Fecal IgA levels in mice inoculated with attenuated strains of Salmonella Typhimurium |
| title_fullStr |
Fecal IgA levels in mice inoculated with attenuated strains of Salmonella Typhimurium |
| title_full_unstemmed |
Fecal IgA levels in mice inoculated with attenuated strains of Salmonella Typhimurium |
| title_sort |
Fecal IgA levels in mice inoculated with attenuated strains of Salmonella Typhimurium |
| dc.creator.none.fl_str_mv |
Changanaquí, Christian Luna E., Luis Carhuaricra H., Dennis Maturrano H., Lenin Rosadio A., Raúl Changanaquí, Christian Luna E., Luis Carhuaricra H., Dennis Maturrano H., Lenin Rosadio A., Raúl |
| author |
Changanaquí, Christian |
| author_facet |
Changanaquí, Christian Luna E., Luis Carhuaricra H., Dennis Maturrano H., Lenin Rosadio A., Raúl |
| author_role |
author |
| author2 |
Luna E., Luis Carhuaricra H., Dennis Maturrano H., Lenin Rosadio A., Raúl |
| author2_role |
author author author author |
| dc.subject.none.fl_str_mv |
attenuated strain Cavia porcellus immunogenicity immunoglobulin A Salmonella Typhimurium cepa atenuada Cavia porcellus inmunogenicidad inmunoglobulina A Salmonella Typhimurium |
| topic |
attenuated strain Cavia porcellus immunogenicity immunoglobulin A Salmonella Typhimurium cepa atenuada Cavia porcellus inmunogenicidad inmunoglobulina A Salmonella Typhimurium |
| description |
The present study aimed to evaluate the immunogenic activity of three life attenuated strains of Salmonella Typhimurium isolated from guinea pig, by evaluating the excretion of faecal IgA in mice. The study was carried out with 28 four-week-old female mice of the Balb/c strain, distributed into four groups (seven mice per group): strain 1, strain 2, strain 3 and control group (PBS). Each mouse was inoculated with 109 CFU/100 µl of the attenuated strains using an orogastric probe. Fresh stool samples were collected at days 1, 9, 15 and 21 post-immunizations. The quantification of faecal IgA was carried out by an indirect in-house ELISA test. The results indicate that the mice inoculated with strains 1 and 2 presented a higher immunogenic activity (faecal IgA) compared to the Control group. |
| publishDate |
2022 |
| dc.date.none.fl_str_mv |
2022-06-29 |
| dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
| format |
article |
| status_str |
publishedVersion |
| dc.identifier.none.fl_str_mv |
https://revistasinvestigacion.unmsm.edu.pe/index.php/veterinaria/article/view/22898 10.15381/rivep.v33i3.22898 |
| url |
https://revistasinvestigacion.unmsm.edu.pe/index.php/veterinaria/article/view/22898 |
| identifier_str_mv |
10.15381/rivep.v33i3.22898 |
| dc.language.none.fl_str_mv |
spa |
| language |
spa |
| dc.relation.none.fl_str_mv |
https://revistasinvestigacion.unmsm.edu.pe/index.php/veterinaria/article/view/22898/18200 |
| dc.rights.none.fl_str_mv |
http://creativecommons.org/licenses/by/4.0 info:eu-repo/semantics/openAccess |
| rights_invalid_str_mv |
http://creativecommons.org/licenses/by/4.0 |
| eu_rights_str_mv |
openAccess |
| dc.format.none.fl_str_mv |
application/pdf |
| dc.publisher.none.fl_str_mv |
Universidad Nacional Mayor de San Marcos, Facultad de Medicina Veterinaria |
| publisher.none.fl_str_mv |
Universidad Nacional Mayor de San Marcos, Facultad de Medicina Veterinaria |
| dc.source.none.fl_str_mv |
Revista de Investigaciones Veterinarias del Perú; Vol. 33 Núm. 3 (2022); e22898 Revista de Investigaciones Veterinarias del Perú; Vol. 33 No. 3 (2022); e22898 1682-3419 1609-9117 reponame:Revistas - Universidad Nacional Mayor de San Marcos instname:Universidad Nacional Mayor de San Marcos instacron:UNMSM |
| instname_str |
Universidad Nacional Mayor de San Marcos |
| instacron_str |
UNMSM |
| institution |
UNMSM |
| reponame_str |
Revistas - Universidad Nacional Mayor de San Marcos |
| collection |
Revistas - Universidad Nacional Mayor de San Marcos |
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13.887938 |
Nota importante:
La información contenida en este registro es de entera responsabilidad de la institución que gestiona el repositorio institucional donde esta contenido este documento o set de datos. El CONCYTEC no se hace responsable por los contenidos (publicaciones y/o datos) accesibles a través del Repositorio Nacional Digital de Ciencia, Tecnología e Innovación de Acceso Abierto (ALICIA).
La información contenida en este registro es de entera responsabilidad de la institución que gestiona el repositorio institucional donde esta contenido este documento o set de datos. El CONCYTEC no se hace responsable por los contenidos (publicaciones y/o datos) accesibles a través del Repositorio Nacional Digital de Ciencia, Tecnología e Innovación de Acceso Abierto (ALICIA).
