MYCOBACTERIUM AVIUM SUBSP. PARATUBERCULOSIS IN DAIRY CATTLE IN THE LIMA AREA DETECTED THROUGH THREE DIAGNOSTIC TECHNIQUES
Descripción del Articulo
The objective of the study was to determine the presence of Mycobacterium avium subsp. paratuberculosis (Map) in dairy cattle in Lima, Peru. The Intradermal Tuberculin Test was applied to 60 cows from three herds and serum samples were tested using two commercial ELISA kits for antibodies against Ma...
| Autores: | , , , |
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| Formato: | artículo |
| Fecha de Publicación: | 2011 |
| Institución: | Universidad Nacional Mayor de San Marcos |
| Repositorio: | Revistas - Universidad Nacional Mayor de San Marcos |
| Lenguaje: | español |
| OAI Identifier: | oai:ojs.csi.unmsm:article/339 |
| Enlace del recurso: | https://revistasinvestigacion.unmsm.edu.pe/index.php/veterinaria/article/view/339 |
| Nivel de acceso: | acceso abierto |
| Materia: | Paratuberculosis enfermedad de Johne Mycobacterium avium subsp. paratuberculosis bacteria ácido alcohol resistente paratuberculosis Johne’s disease Mycobacterium avium subsp acid-fast bacteria |
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MYCOBACTERIUM AVIUM SUBSP. PARATUBERCULOSIS IN DAIRY CATTLE IN THE LIMA AREA DETECTED THROUGH THREE DIAGNOSTIC TECHNIQUESMycobacterium avium SUBSP. paratuberculosis EN BOVINOS LECHEROS DE LA ZONA DE LIMA DETECTADO MEDIANTE TRES TÉCNICAS DIAGNÓSTICASBustamante V., JoséAguilar O., JoséOrtiz M., MartínBustamante L., JoséParatuberculosisenfermedad de JohneMycobacterium avium subsp. paratuberculosisbacteria ácido alcohol resistenteparatuberculosisJohne’s diseaseMycobacterium avium subspacid-fast bacteriaThe objective of the study was to determine the presence of Mycobacterium avium subsp. paratuberculosis (Map) in dairy cattle in Lima, Peru. The Intradermal Tuberculin Test was applied to 60 cows from three herds and serum samples were tested using two commercial ELISA kits for antibodies against Map. Twenty-two cows (36.7%) were positive to at least to one of these diagnostic techniques (8 to one of the ELISA tests and 18 to the intradermal test). Feacal samples from the 22 positive animals were cultured on Herrold’s egg-yolk medium (HEYM) yielding five Map positive samples. PCR was used in the isolated strains and Map DNA was confirmed in two cows. A slight concordance (0.15, Kappa test) was found between techniques evaluating cellular and humoral immune response, establishing that these tests are not interchangeable (5.55> 3.84, Mc Nemar test). The results confirm the presence of Map in dairy cattle in Lima, Peru.El presente estudio tuvo como objetivo evidenciar la presencia de Mycobacterium avium subsp. paratuberculosis (Map), en el ganado bovino lechero del departamento de Lima, Perú. Se evaluaron 60 vacas procedentes de tres establos, mediante la prueba tuberculínica intradérmica y dos kits comerciales de ELISA específicos contra Mycobacterium avium subsp. paratuberculosis. Resultaron positivos 22 animales (36.7%) a una de las tres técnicas diagnósticas (8 a una de las pruebas de ELISA y 18 a la prueba intradérmica). Se tomó muestras de heces a los 22 animales positivos y se cultivaron en el medio de Herrold con yema de huevo, resultando cinco muestras positivas al aislamiento de Map. Asimismo, se realizó el PCR a partir del cultivo, encontrándose dos muestras positivas. Se encontró una concordancia ligera (0.15, prueba Kappa) entre las técnicas que evalúan la respuesta inmune celular y humoral y se estableció que estas pruebas no son reemplazables (5.55>3.84, prueba de Mc Nemar). Los resultados permitieron evidenciar la presencia de Map en ganado bovino lechero de Lima, Perú.Universidad Nacional Mayor de San Marcos, Facultad de Medicina Veterinaria2011-12-30info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://revistasinvestigacion.unmsm.edu.pe/index.php/veterinaria/article/view/33910.15381/rivep.v22i4.339Revista de Investigaciones Veterinarias del Perú; Vol. 22 Núm. 4 (2011); 394-402Revista de Investigaciones Veterinarias del Perú; Vol. 22 No. 4 (2011); 394-4021682-34191609-9117reponame:Revistas - Universidad Nacional Mayor de San Marcosinstname:Universidad Nacional Mayor de San Marcosinstacron:UNMSMspahttps://revistasinvestigacion.unmsm.edu.pe/index.php/veterinaria/article/view/339/299Derechos de autor 2011 José Bustamante V., José Aguilar O., Martín Ortiz M., José Bustamante L.https://creativecommons.org/licenses/by-nc-sa/4.0info:eu-repo/semantics/openAccessoai:ojs.csi.unmsm:article/3392020-03-19T18:25:16Z |
| dc.title.none.fl_str_mv |
MYCOBACTERIUM AVIUM SUBSP. PARATUBERCULOSIS IN DAIRY CATTLE IN THE LIMA AREA DETECTED THROUGH THREE DIAGNOSTIC TECHNIQUES Mycobacterium avium SUBSP. paratuberculosis EN BOVINOS LECHEROS DE LA ZONA DE LIMA DETECTADO MEDIANTE TRES TÉCNICAS DIAGNÓSTICAS |
| title |
MYCOBACTERIUM AVIUM SUBSP. PARATUBERCULOSIS IN DAIRY CATTLE IN THE LIMA AREA DETECTED THROUGH THREE DIAGNOSTIC TECHNIQUES |
| spellingShingle |
MYCOBACTERIUM AVIUM SUBSP. PARATUBERCULOSIS IN DAIRY CATTLE IN THE LIMA AREA DETECTED THROUGH THREE DIAGNOSTIC TECHNIQUES Bustamante V., José Paratuberculosis enfermedad de Johne Mycobacterium avium subsp. paratuberculosis bacteria ácido alcohol resistente paratuberculosis Johne’s disease Mycobacterium avium subsp acid-fast bacteria |
| title_short |
MYCOBACTERIUM AVIUM SUBSP. PARATUBERCULOSIS IN DAIRY CATTLE IN THE LIMA AREA DETECTED THROUGH THREE DIAGNOSTIC TECHNIQUES |
| title_full |
MYCOBACTERIUM AVIUM SUBSP. PARATUBERCULOSIS IN DAIRY CATTLE IN THE LIMA AREA DETECTED THROUGH THREE DIAGNOSTIC TECHNIQUES |
| title_fullStr |
MYCOBACTERIUM AVIUM SUBSP. PARATUBERCULOSIS IN DAIRY CATTLE IN THE LIMA AREA DETECTED THROUGH THREE DIAGNOSTIC TECHNIQUES |
| title_full_unstemmed |
MYCOBACTERIUM AVIUM SUBSP. PARATUBERCULOSIS IN DAIRY CATTLE IN THE LIMA AREA DETECTED THROUGH THREE DIAGNOSTIC TECHNIQUES |
| title_sort |
MYCOBACTERIUM AVIUM SUBSP. PARATUBERCULOSIS IN DAIRY CATTLE IN THE LIMA AREA DETECTED THROUGH THREE DIAGNOSTIC TECHNIQUES |
| dc.creator.none.fl_str_mv |
Bustamante V., José Aguilar O., José Ortiz M., Martín Bustamante L., José |
| author |
Bustamante V., José |
| author_facet |
Bustamante V., José Aguilar O., José Ortiz M., Martín Bustamante L., José |
| author_role |
author |
| author2 |
Aguilar O., José Ortiz M., Martín Bustamante L., José |
| author2_role |
author author author |
| dc.subject.none.fl_str_mv |
Paratuberculosis enfermedad de Johne Mycobacterium avium subsp. paratuberculosis bacteria ácido alcohol resistente paratuberculosis Johne’s disease Mycobacterium avium subsp acid-fast bacteria |
| topic |
Paratuberculosis enfermedad de Johne Mycobacterium avium subsp. paratuberculosis bacteria ácido alcohol resistente paratuberculosis Johne’s disease Mycobacterium avium subsp acid-fast bacteria |
| description |
The objective of the study was to determine the presence of Mycobacterium avium subsp. paratuberculosis (Map) in dairy cattle in Lima, Peru. The Intradermal Tuberculin Test was applied to 60 cows from three herds and serum samples were tested using two commercial ELISA kits for antibodies against Map. Twenty-two cows (36.7%) were positive to at least to one of these diagnostic techniques (8 to one of the ELISA tests and 18 to the intradermal test). Feacal samples from the 22 positive animals were cultured on Herrold’s egg-yolk medium (HEYM) yielding five Map positive samples. PCR was used in the isolated strains and Map DNA was confirmed in two cows. A slight concordance (0.15, Kappa test) was found between techniques evaluating cellular and humoral immune response, establishing that these tests are not interchangeable (5.55> 3.84, Mc Nemar test). The results confirm the presence of Map in dairy cattle in Lima, Peru. |
| publishDate |
2011 |
| dc.date.none.fl_str_mv |
2011-12-30 |
| dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
| format |
article |
| status_str |
publishedVersion |
| dc.identifier.none.fl_str_mv |
https://revistasinvestigacion.unmsm.edu.pe/index.php/veterinaria/article/view/339 10.15381/rivep.v22i4.339 |
| url |
https://revistasinvestigacion.unmsm.edu.pe/index.php/veterinaria/article/view/339 |
| identifier_str_mv |
10.15381/rivep.v22i4.339 |
| dc.language.none.fl_str_mv |
spa |
| language |
spa |
| dc.relation.none.fl_str_mv |
https://revistasinvestigacion.unmsm.edu.pe/index.php/veterinaria/article/view/339/299 |
| dc.rights.none.fl_str_mv |
Derechos de autor 2011 José Bustamante V., José Aguilar O., Martín Ortiz M., José Bustamante L. https://creativecommons.org/licenses/by-nc-sa/4.0 info:eu-repo/semantics/openAccess |
| rights_invalid_str_mv |
Derechos de autor 2011 José Bustamante V., José Aguilar O., Martín Ortiz M., José Bustamante L. https://creativecommons.org/licenses/by-nc-sa/4.0 |
| eu_rights_str_mv |
openAccess |
| dc.format.none.fl_str_mv |
application/pdf |
| dc.publisher.none.fl_str_mv |
Universidad Nacional Mayor de San Marcos, Facultad de Medicina Veterinaria |
| publisher.none.fl_str_mv |
Universidad Nacional Mayor de San Marcos, Facultad de Medicina Veterinaria |
| dc.source.none.fl_str_mv |
Revista de Investigaciones Veterinarias del Perú; Vol. 22 Núm. 4 (2011); 394-402 Revista de Investigaciones Veterinarias del Perú; Vol. 22 No. 4 (2011); 394-402 1682-3419 1609-9117 reponame:Revistas - Universidad Nacional Mayor de San Marcos instname:Universidad Nacional Mayor de San Marcos instacron:UNMSM |
| instname_str |
Universidad Nacional Mayor de San Marcos |
| instacron_str |
UNMSM |
| institution |
UNMSM |
| reponame_str |
Revistas - Universidad Nacional Mayor de San Marcos |
| collection |
Revistas - Universidad Nacional Mayor de San Marcos |
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13.754011 |
Nota importante:
La información contenida en este registro es de entera responsabilidad de la institución que gestiona el repositorio institucional donde esta contenido este documento o set de datos. El CONCYTEC no se hace responsable por los contenidos (publicaciones y/o datos) accesibles a través del Repositorio Nacional Digital de Ciencia, Tecnología e Innovación de Acceso Abierto (ALICIA).
La información contenida en este registro es de entera responsabilidad de la institución que gestiona el repositorio institucional donde esta contenido este documento o set de datos. El CONCYTEC no se hace responsable por los contenidos (publicaciones y/o datos) accesibles a través del Repositorio Nacional Digital de Ciencia, Tecnología e Innovación de Acceso Abierto (ALICIA).
