Caracterización fenotípica y genotípica de factores de virulencia de cepas de Escherichia coli uropatógena productora de carbapenemasa

• Autores: Ramos-Nolazco, Gabriela Alessandra, Coral-Clavijo, Sebastián Alberto, Gonzales Escalante, Edgar, Egoavil-Rosales, Rocio del Pilar
• Formato: artículo
• Fecha de Publicación: 2025
• Institución: Universidad Nacional Mayor de San Marcos
• Repositorio: Revistas - Universidad Nacional Mayor de San Marcos
• Lenguaje: español
• OAI Identifier: oai:revistasinvestigacion.unmsm.edu.pe:article/31563
• Enlace del recurso: https://revistasinvestigacion.unmsm.edu.pe/index.php/anales/article/view/31563
• Nivel de acceso: acceso abierto
• Materia: Uropathogenic Escherichia coli; Carbapenem-Resistant Enterobacteriaceae; Virulence Factors; Escherichia coli Uropatógena; Farmacorresistencia Bacteriana; Factores de virulencia; Enterobacteriaceae Resistentes a los Carbapenémicos

Introduction. Uropathogenic Escherichia coli (UPEC) is the leading cause of urinary tract infections (UTIs). Its carbapenem-resistant form (UPEC-rC) harbors specific virulence factors that further limit therapeutic options. Objective. Identify virulence factors in UPEC-rC isolates recovered from urine samples of patients with UTIs in Lima, Peru. Methods. We conducted a descriptive observational study including 58 UPEC-rC isolates. Virulence factors were analyzed in the laboratory: phenotypically (hemolysin on blood agar, P fimbriae by erythrocyte hemagglutination and biofilms production using the microtiter plate method) and genotypically (α-hly, papGII, aer, cnf1, sfa/focDE) by PCR. Results. 28 out of 58 isolates exhibited at least one virulence factor. Genotypically, aer gene was the most frequent (34,5%), while phenotypically, biofilm formation predominated (17,2%). Conclusion. The frequency of aer gen and biofilm production suggests a possible relationship between carbapenem resistance and the attenuation of pathogenic mechanisms.