GENETIC VARIABILITY OF Gallibacterium anatis STRAINS ISOLATED FROM PERUVIAN COMMERCIAL BIRDS WITH RESPIRATORY INFECTIONS

Descripción del Articulo

The purpose of this study was to determine the genetic variability of strains of Gallibacterium anatis isolated in commercial poultry with respiratory symptoms from Arequipa (2), Ica (3), La Libertad (27), Lima (62), Madre de Dios (1) and Ucayali (1), collected from 2007 to 2011. Strains were typifi...

Descripción completa

Detalles Bibliográficos
Autores: Mendoza A., Karina, Zavaleta, Amparo I., Koga, Ysabel, Rodríguez B., Jorge, Alvarado S., Arnaldo, Tinico R., Robert
Formato: artículo
Fecha de Publicación:2014
Institución:Universidad Nacional Mayor de San Marcos
Repositorio:Revistas - Universidad Nacional Mayor de San Marcos
Lenguaje:español
OAI Identifier:oai:ojs.csi.unmsm:article/8496
Enlace del recurso:https://revistasinvestigacion.unmsm.edu.pe/index.php/veterinaria/article/view/8496
Nivel de acceso:acceso abierto
Materia:Variabilidad genética
Gallibacterium anatis
aves comerciales
ERICPCR
biovares
Perú.
genetic variability
commercial poultry
ERIC-PCR
biovar
Peru
id REVUNMSM_94f993a9b79728542d6efe2b1b27bbad
oai_identifier_str oai:ojs.csi.unmsm:article/8496
network_acronym_str REVUNMSM
network_name_str Revistas - Universidad Nacional Mayor de San Marcos
repository_id_str
spelling GENETIC VARIABILITY OF Gallibacterium anatis STRAINS ISOLATED FROM PERUVIAN COMMERCIAL BIRDS WITH RESPIRATORY INFECTIONSVARIABILIDAD GENÉTICA DE CEPAS DE Gallibacterium anatis AISLADAS DE AVES COMERCIALES DEL PERÚ CON INFECCIONES RESPIRATORIAS.Mendoza A., KarinaZavaleta, Amparo I.Koga, YsabelRodríguez B., JorgeAlvarado S., ArnaldoTinico R., RobertVariabilidad genéticaGallibacterium anatisaves comercialesERICPCRbiovaresPerú.genetic variabilityGallibacterium anatiscommercial poultryERIC-PCRbiovarPeruThe purpose of this study was to determine the genetic variability of strains of Gallibacterium anatis isolated in commercial poultry with respiratory symptoms from Arequipa (2), Ica (3), La Libertad (27), Lima (62), Madre de Dios (1) and Ucayali (1), collected from 2007 to 2011. Strains were typified by carbohydrate fermentation tests and sensitivity to nine antimicrobials. The genetic variability of these strains was determined by ERIC-PCR, after identification by PCR using specific primers. The analysis of carbohydrate fermentation profiles corresponded to 10 biovars, all of which were found in Lima and 45.8% of the strains belonged to biovar 4. Concerning antimicrobial susceptibility, 40.8% of strains were resistant to all antimicrobials studied, while 94.8 and 95.8% were resistant to enrofloxacin and ciprofloxacin. By ERIC-PCR 24 DNA profiles associated with biovars were obtained, indicating the existence of intraspecific genetic variability in strains of G. anatis circulating in Peru.El propósito del estudio fue determinar la variabilidad genética de 96 cepas de Gallibacterium anatis aisladas de aves comerciales con sintomatología respiratoria, provenientes de Arequipa (2), Ica (3), La Libertad (27), Lima (62), Madre de Dios (1) y Ucayali (1), y recolectadas desde el 2007 al 2011. Las cepas se tipificaron mediante pruebas de fermentación de carbohidratos y sensibilidad a nueve antimicrobianos. La variabilidad genética de las cepas de G. anatis se determinó mediante ERIC-PCR, previa identificación por PCR usando cebadores específicos. Del análisis de fermentación de carbohidratos se obtuvieron perfiles que correspondieron a 10 biovares, todos los cuales se encontraron en Lima y el 45.8% de las cepas pertenecieron al biovar 4. Con respecto a la sensibilidad antimicrobiana, el 40.8% de las cepas fueron resistentes a todos los antimicrobianos estudiados, en tanto que el 94.8 y 95.8% fueron resistentes a enrofloxacina y ciprofloxacina. Por ERIC-PCR se obtuvieron 24 perfiles de ADN no asociados con los biovares, lo cual indica la existencia de variabilidad genética intraespecífica en cepas de G. anatis circulantes en Perú.Universidad Nacional Mayor de San Marcos, Facultad de Medicina Veterinaria2014-06-17info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://revistasinvestigacion.unmsm.edu.pe/index.php/veterinaria/article/view/849610.15381/rivep.v25i2.8496Revista de Investigaciones Veterinarias del Perú; Vol. 25 Núm. 2 (2014); 233-244Revista de Investigaciones Veterinarias del Perú; Vol. 25 No. 2 (2014); 233-2441682-34191609-9117reponame:Revistas - Universidad Nacional Mayor de San Marcosinstname:Universidad Nacional Mayor de San Marcosinstacron:UNMSMspahttps://revistasinvestigacion.unmsm.edu.pe/index.php/veterinaria/article/view/8496/7371Derechos de autor 2014 Karina Mendoza A., Amparo I. Zavaleta, Ysabel Koga, Jorge Rodríguez B., Arnaldo Alvarado S., Robert Tinico R.https://creativecommons.org/licenses/by-nc-sa/4.0info:eu-repo/semantics/openAccessoai:ojs.csi.unmsm:article/84962020-03-29T17:55:11Z
dc.title.none.fl_str_mv GENETIC VARIABILITY OF Gallibacterium anatis STRAINS ISOLATED FROM PERUVIAN COMMERCIAL BIRDS WITH RESPIRATORY INFECTIONS
VARIABILIDAD GENÉTICA DE CEPAS DE Gallibacterium anatis AISLADAS DE AVES COMERCIALES DEL PERÚ CON INFECCIONES RESPIRATORIAS.
title GENETIC VARIABILITY OF Gallibacterium anatis STRAINS ISOLATED FROM PERUVIAN COMMERCIAL BIRDS WITH RESPIRATORY INFECTIONS
spellingShingle GENETIC VARIABILITY OF Gallibacterium anatis STRAINS ISOLATED FROM PERUVIAN COMMERCIAL BIRDS WITH RESPIRATORY INFECTIONS
Mendoza A., Karina
Variabilidad genética
Gallibacterium anatis
aves comerciales
ERICPCR
biovares
Perú.
genetic variability
Gallibacterium anatis
commercial poultry
ERIC-PCR
biovar
Peru
title_short GENETIC VARIABILITY OF Gallibacterium anatis STRAINS ISOLATED FROM PERUVIAN COMMERCIAL BIRDS WITH RESPIRATORY INFECTIONS
title_full GENETIC VARIABILITY OF Gallibacterium anatis STRAINS ISOLATED FROM PERUVIAN COMMERCIAL BIRDS WITH RESPIRATORY INFECTIONS
title_fullStr GENETIC VARIABILITY OF Gallibacterium anatis STRAINS ISOLATED FROM PERUVIAN COMMERCIAL BIRDS WITH RESPIRATORY INFECTIONS
title_full_unstemmed GENETIC VARIABILITY OF Gallibacterium anatis STRAINS ISOLATED FROM PERUVIAN COMMERCIAL BIRDS WITH RESPIRATORY INFECTIONS
title_sort GENETIC VARIABILITY OF Gallibacterium anatis STRAINS ISOLATED FROM PERUVIAN COMMERCIAL BIRDS WITH RESPIRATORY INFECTIONS
dc.creator.none.fl_str_mv Mendoza A., Karina
Zavaleta, Amparo I.
Koga, Ysabel
Rodríguez B., Jorge
Alvarado S., Arnaldo
Tinico R., Robert
author Mendoza A., Karina
author_facet Mendoza A., Karina
Zavaleta, Amparo I.
Koga, Ysabel
Rodríguez B., Jorge
Alvarado S., Arnaldo
Tinico R., Robert
author_role author
author2 Zavaleta, Amparo I.
Koga, Ysabel
Rodríguez B., Jorge
Alvarado S., Arnaldo
Tinico R., Robert
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv Variabilidad genética
Gallibacterium anatis
aves comerciales
ERICPCR
biovares
Perú.
genetic variability
Gallibacterium anatis
commercial poultry
ERIC-PCR
biovar
Peru
topic Variabilidad genética
Gallibacterium anatis
aves comerciales
ERICPCR
biovares
Perú.
genetic variability
Gallibacterium anatis
commercial poultry
ERIC-PCR
biovar
Peru
description The purpose of this study was to determine the genetic variability of strains of Gallibacterium anatis isolated in commercial poultry with respiratory symptoms from Arequipa (2), Ica (3), La Libertad (27), Lima (62), Madre de Dios (1) and Ucayali (1), collected from 2007 to 2011. Strains were typified by carbohydrate fermentation tests and sensitivity to nine antimicrobials. The genetic variability of these strains was determined by ERIC-PCR, after identification by PCR using specific primers. The analysis of carbohydrate fermentation profiles corresponded to 10 biovars, all of which were found in Lima and 45.8% of the strains belonged to biovar 4. Concerning antimicrobial susceptibility, 40.8% of strains were resistant to all antimicrobials studied, while 94.8 and 95.8% were resistant to enrofloxacin and ciprofloxacin. By ERIC-PCR 24 DNA profiles associated with biovars were obtained, indicating the existence of intraspecific genetic variability in strains of G. anatis circulating in Peru.
publishDate 2014
dc.date.none.fl_str_mv 2014-06-17
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://revistasinvestigacion.unmsm.edu.pe/index.php/veterinaria/article/view/8496
10.15381/rivep.v25i2.8496
url https://revistasinvestigacion.unmsm.edu.pe/index.php/veterinaria/article/view/8496
identifier_str_mv 10.15381/rivep.v25i2.8496
dc.language.none.fl_str_mv spa
language spa
dc.relation.none.fl_str_mv https://revistasinvestigacion.unmsm.edu.pe/index.php/veterinaria/article/view/8496/7371
dc.rights.none.fl_str_mv https://creativecommons.org/licenses/by-nc-sa/4.0
info:eu-repo/semantics/openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/4.0
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Universidad Nacional Mayor de San Marcos, Facultad de Medicina Veterinaria
publisher.none.fl_str_mv Universidad Nacional Mayor de San Marcos, Facultad de Medicina Veterinaria
dc.source.none.fl_str_mv Revista de Investigaciones Veterinarias del Perú; Vol. 25 Núm. 2 (2014); 233-244
Revista de Investigaciones Veterinarias del Perú; Vol. 25 No. 2 (2014); 233-244
1682-3419
1609-9117
reponame:Revistas - Universidad Nacional Mayor de San Marcos
instname:Universidad Nacional Mayor de San Marcos
instacron:UNMSM
instname_str Universidad Nacional Mayor de San Marcos
instacron_str UNMSM
institution UNMSM
reponame_str Revistas - Universidad Nacional Mayor de San Marcos
collection Revistas - Universidad Nacional Mayor de San Marcos
repository.name.fl_str_mv
repository.mail.fl_str_mv
_version_ 1795238225670832128
score 13.904524
GENETIC VARIABILITY OF Gallibacterium anatis STRAINS ISOLATED FROM PERUVIAN COMMERCIAL BIRDS WITH RESPIRATORY INFECTIONS
Nota importante:
La información contenida en este registro es de entera responsabilidad de la institución que gestiona el repositorio institucional donde esta contenido este documento o set de datos. El CONCYTEC no se hace responsable por los contenidos (publicaciones y/o datos) accesibles a través del Repositorio Nacional Digital de Ciencia, Tecnología e Innovación de Acceso Abierto (ALICIA).

Ejemplares Similares