Characterization of microorganisms with probiotic potential isolated from Brahman calf manure in Sucre, Colombia
Descripción del Articulo
The objective of the study was to characterize microorganisms with probiotic potential of manure of lactating Brahman calves in Sucre (Colombia). Bacteria and yeasts were isolated from manure samples and the probiotic capacity of these strains was determined by bile salt resistance test (0.05, 0.1,...
| Autores: | , , |
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| Formato: | artículo |
| Fecha de Publicación: | 2018 |
| Institución: | Universidad Nacional Mayor de San Marcos |
| Repositorio: | Revistas - Universidad Nacional Mayor de San Marcos |
| Lenguaje: | español |
| OAI Identifier: | oai:ojs.csi.unmsm:article/14482 |
| Enlace del recurso: | https://revistasinvestigacion.unmsm.edu.pe/index.php/veterinaria/article/view/14482 |
| Nivel de acceso: | acceso abierto |
| Materia: | probiotic cattle zebu Enterococcus antagonistic capacity stains probióticos ganado cebú capacidad antagónica cepa |
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Characterization of microorganisms with probiotic potential isolated from Brahman calf manure in Sucre, ColombiaCaracterización de microorganismos con potencial probiótico aislados de estiércol de terneros Brahman en Sucre, ColombiaCastillo Arroyo, Pedro LuisBetancur Hurtado, César AugustoPardo Pérez, EnriqueprobioticcattlezebuEnterococcusantagonistic capacitystainsprobióticosganadocebúEnterococcuscapacidad antagónicacepaThe objective of the study was to characterize microorganisms with probiotic potential of manure of lactating Brahman calves in Sucre (Colombia). Bacteria and yeasts were isolated from manure samples and the probiotic capacity of these strains was determined by bile salt resistance test (0.05, 0.1, 0.15, 0.3%), resistance to acidic pH (pH 3, 4, 5.6, 7 ), tolerance to NaCl (2, 4, 6, 8, 10%) and antagonistic activity (Salmonella sp and Escherichia coli). Nine microorganisms were identified and three passed the tolerance tests. The antagonistic capacity was determined against pathogenic bacteria (Salmonella sp and E. coli), evidenced by halos (mm) of the three selected strains. The identification of the strains was carried out by the biochemical methods API 50 CHL V5.1 for Lactobacillus and API 20 AUX for yeasts. Molecular analysis of two strains (M13a and 103M2) identified strain M13a as Enterococcus faecium and strain 103M2 as Candida krusei. In conclusion, Enterococcus faecium becomes a viable alternative for the formulation of a biopreparation to improve the productive parameters and decrease gastrointestinal disorders in lactating calves.El objetivo del estudio fue caracterizar microorganismos con potencial probiótico de estiércol de terneros lactantes Brahman en Sucre (Colombia). Se aislaron bacterias y levaduras de las muestras de estiércol y se determinó la capacidad probiótica de estas cepas mediante pruebas de resistencia a sales biliares (0.05, 0.1, 0.15 y 0.3%), resistencia a pH ácido (pH 3, 4, 5.6, 7), tolerancia a NaCl (2, 4, 6, 8, 10%) y actividad antagónica (Salmonella sp y Escherichia coli). Nueve microorganismos fueron identificados y tres pasaron las pruebas de tolerancia. Se determinó la capacidad antagónica frente a bacterias patógenas (Salmonella sp y E. coli), evidenciada por halos (mm) de las tres cepas seleccionadas. La identificación de las cepas se realizó por los métodos bioquímicos API 50 CHL V5.1 para Lactobacillus y API 20 AUX para levaduras. El análisis molecular de dos cepas (M13a y 103M2) identificó la cepa M13a como Enterococcus faecium y la cepa 103M2 como Candida krusei. En conclusión, Enterococcus faecium se convierte en una alternativa viable para la formulación de un biopreparado para mejorar los parámetros productivos y disminuir los trastornos gastrointestinales en los terneros lactantes.Universidad Nacional Mayor de San Marcos, Facultad de Medicina Veterinaria2018-05-31info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdftext/htmlhttps://revistasinvestigacion.unmsm.edu.pe/index.php/veterinaria/article/view/1448210.15381/rivep.v29i2.14482Revista de Investigaciones Veterinarias del Perú; Vol. 29 Núm. 2 (2018); 438-448Revista de Investigaciones Veterinarias del Perú; Vol. 29 No. 2 (2018); 438-4481682-34191609-9117reponame:Revistas - Universidad Nacional Mayor de San Marcosinstname:Universidad Nacional Mayor de San Marcosinstacron:UNMSMspahttps://revistasinvestigacion.unmsm.edu.pe/index.php/veterinaria/article/view/14482/12822https://revistasinvestigacion.unmsm.edu.pe/index.php/veterinaria/article/view/14482/13942Derechos de autor 2018 Pedro Luis Castillo Arroyo, César Augusto Betancur Hurtado, Enrique Pardo Pérezhttps://creativecommons.org/licenses/by-nc-sa/4.0info:eu-repo/semantics/openAccessoai:ojs.csi.unmsm:article/144822018-12-21T09:58:48Z |
| dc.title.none.fl_str_mv |
Characterization of microorganisms with probiotic potential isolated from Brahman calf manure in Sucre, Colombia Caracterización de microorganismos con potencial probiótico aislados de estiércol de terneros Brahman en Sucre, Colombia |
| title |
Characterization of microorganisms with probiotic potential isolated from Brahman calf manure in Sucre, Colombia |
| spellingShingle |
Characterization of microorganisms with probiotic potential isolated from Brahman calf manure in Sucre, Colombia Castillo Arroyo, Pedro Luis probiotic cattle zebu Enterococcus antagonistic capacity stains probióticos ganado cebú Enterococcus capacidad antagónica cepa |
| title_short |
Characterization of microorganisms with probiotic potential isolated from Brahman calf manure in Sucre, Colombia |
| title_full |
Characterization of microorganisms with probiotic potential isolated from Brahman calf manure in Sucre, Colombia |
| title_fullStr |
Characterization of microorganisms with probiotic potential isolated from Brahman calf manure in Sucre, Colombia |
| title_full_unstemmed |
Characterization of microorganisms with probiotic potential isolated from Brahman calf manure in Sucre, Colombia |
| title_sort |
Characterization of microorganisms with probiotic potential isolated from Brahman calf manure in Sucre, Colombia |
| dc.creator.none.fl_str_mv |
Castillo Arroyo, Pedro Luis Betancur Hurtado, César Augusto Pardo Pérez, Enrique |
| author |
Castillo Arroyo, Pedro Luis |
| author_facet |
Castillo Arroyo, Pedro Luis Betancur Hurtado, César Augusto Pardo Pérez, Enrique |
| author_role |
author |
| author2 |
Betancur Hurtado, César Augusto Pardo Pérez, Enrique |
| author2_role |
author author |
| dc.subject.none.fl_str_mv |
probiotic cattle zebu Enterococcus antagonistic capacity stains probióticos ganado cebú Enterococcus capacidad antagónica cepa |
| topic |
probiotic cattle zebu Enterococcus antagonistic capacity stains probióticos ganado cebú Enterococcus capacidad antagónica cepa |
| description |
The objective of the study was to characterize microorganisms with probiotic potential of manure of lactating Brahman calves in Sucre (Colombia). Bacteria and yeasts were isolated from manure samples and the probiotic capacity of these strains was determined by bile salt resistance test (0.05, 0.1, 0.15, 0.3%), resistance to acidic pH (pH 3, 4, 5.6, 7 ), tolerance to NaCl (2, 4, 6, 8, 10%) and antagonistic activity (Salmonella sp and Escherichia coli). Nine microorganisms were identified and three passed the tolerance tests. The antagonistic capacity was determined against pathogenic bacteria (Salmonella sp and E. coli), evidenced by halos (mm) of the three selected strains. The identification of the strains was carried out by the biochemical methods API 50 CHL V5.1 for Lactobacillus and API 20 AUX for yeasts. Molecular analysis of two strains (M13a and 103M2) identified strain M13a as Enterococcus faecium and strain 103M2 as Candida krusei. In conclusion, Enterococcus faecium becomes a viable alternative for the formulation of a biopreparation to improve the productive parameters and decrease gastrointestinal disorders in lactating calves. |
| publishDate |
2018 |
| dc.date.none.fl_str_mv |
2018-05-31 |
| dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
| format |
article |
| status_str |
publishedVersion |
| dc.identifier.none.fl_str_mv |
https://revistasinvestigacion.unmsm.edu.pe/index.php/veterinaria/article/view/14482 10.15381/rivep.v29i2.14482 |
| url |
https://revistasinvestigacion.unmsm.edu.pe/index.php/veterinaria/article/view/14482 |
| identifier_str_mv |
10.15381/rivep.v29i2.14482 |
| dc.language.none.fl_str_mv |
spa |
| language |
spa |
| dc.relation.none.fl_str_mv |
https://revistasinvestigacion.unmsm.edu.pe/index.php/veterinaria/article/view/14482/12822 https://revistasinvestigacion.unmsm.edu.pe/index.php/veterinaria/article/view/14482/13942 |
| dc.rights.none.fl_str_mv |
https://creativecommons.org/licenses/by-nc-sa/4.0 info:eu-repo/semantics/openAccess |
| rights_invalid_str_mv |
https://creativecommons.org/licenses/by-nc-sa/4.0 |
| eu_rights_str_mv |
openAccess |
| dc.format.none.fl_str_mv |
application/pdf text/html |
| dc.publisher.none.fl_str_mv |
Universidad Nacional Mayor de San Marcos, Facultad de Medicina Veterinaria |
| publisher.none.fl_str_mv |
Universidad Nacional Mayor de San Marcos, Facultad de Medicina Veterinaria |
| dc.source.none.fl_str_mv |
Revista de Investigaciones Veterinarias del Perú; Vol. 29 Núm. 2 (2018); 438-448 Revista de Investigaciones Veterinarias del Perú; Vol. 29 No. 2 (2018); 438-448 1682-3419 1609-9117 reponame:Revistas - Universidad Nacional Mayor de San Marcos instname:Universidad Nacional Mayor de San Marcos instacron:UNMSM |
| instname_str |
Universidad Nacional Mayor de San Marcos |
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UNMSM |
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UNMSM |
| reponame_str |
Revistas - Universidad Nacional Mayor de San Marcos |
| collection |
Revistas - Universidad Nacional Mayor de San Marcos |
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13.87115 |
Nota importante:
La información contenida en este registro es de entera responsabilidad de la institución que gestiona el repositorio institucional donde esta contenido este documento o set de datos. El CONCYTEC no se hace responsable por los contenidos (publicaciones y/o datos) accesibles a través del Repositorio Nacional Digital de Ciencia, Tecnología e Innovación de Acceso Abierto (ALICIA).
La información contenida en este registro es de entera responsabilidad de la institución que gestiona el repositorio institucional donde esta contenido este documento o set de datos. El CONCYTEC no se hace responsable por los contenidos (publicaciones y/o datos) accesibles a través del Repositorio Nacional Digital de Ciencia, Tecnología e Innovación de Acceso Abierto (ALICIA).
