Basic and functional characterization of the Peruvian Hairless Dog semen
Descripción del Articulo
The aim of this study was to characterize the semen of the Peruvian Hairless Dog. Fifteen males, aged 1 to 5 years, registered by the Kennel Club Peruano were used to collect semen (one ejaculated per male) by digital manipulation. A basic spermatogram was performed and macroscopic (volume and colou...
| Autores: | , , |
|---|---|
| Formato: | artículo |
| Fecha de Publicación: | 2020 |
| Institución: | Universidad Nacional Mayor de San Marcos |
| Repositorio: | Revistas - Universidad Nacional Mayor de San Marcos |
| Lenguaje: | español |
| OAI Identifier: | oai:ojs.csi.unmsm:article/19251 |
| Enlace del recurso: | https://revistasinvestigacion.unmsm.edu.pe/index.php/veterinaria/article/view/19251 |
| Nivel de acceso: | acceso abierto |
| Materia: | Peruvian hairless dog semen spermatozoa flow cytometry Perro sin pelo del Perú espermatozoide citometría de flujo |
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Basic and functional characterization of the Peruvian Hairless Dog semenCaracterización básica y funcional del semen del Perro sin Pelo del PerúSantiani, AlexeiPérez- Rosales, MaríaChallco, CarlosPeruvian hairless dogsemenspermatozoaflow cytometryPerro sin pelo del Perúsemenespermatozoidecitometría de flujoThe aim of this study was to characterize the semen of the Peruvian Hairless Dog. Fifteen males, aged 1 to 5 years, registered by the Kennel Club Peruano were used to collect semen (one ejaculated per male) by digital manipulation. A basic spermatogram was performed and macroscopic (volume and colour) and microscopic (sperm motility and concentration, using light field microscopy) characteristics were recorded. Additionally, sperm function tests were performed: sperm viability (using SYBR-14 and propidium iodide), mitochondrial membrane potential (MMP, using MitoTraker Deep Red FM), and acrosomal integrity (using FITC-PSA and propidium iodide) and analysed by flow cytometry with image analysis. The following values were found (mean ± 95% CI): 6.1 ± 2.7 ml volume, opalescent colour; 66.7 ± 10.5% motility; 262.7 ± 20.4 x 106 sperm/ml sperm concentration; 77.7 ± 9.7% of sperm viability; 85.9 ± 5.1% high MMP; 83.6 ± 14.9% acrosomal integrity. These results represent the first report on the seminal characteristics of this breed and could be taken as reference values for a better selection of breeding males and for the diagnosis of reproductive alterations of the breed.El objetivo del estudio fue caracterizar el semen del Perro sin Pelo del Perú. Quince machos, de edades entre 1 a 5 años, inscritos en el Kennel Club Peruano fueron utilizados para colectar semen (un eyaculado por macho) mediante manipulación digital. Se realizó un espermatograma básico y se registraron características macroscópicas (volumen y color) y microscópicas (motilidad y concentración espermática, utilizando microscopía de campo claro). Adicionalmente, se realizaron pruebas de función espermática: viabilidad espermática (utilizando SYBR-14 y ioduro de propidio), potencial de membrana mitocondrial (PMM; utilizando MitoTraker Deep Red FM), e integridad acrosomal (utilizando FITC-PSA y ioduro de propidio), analizadas posteriormente mediante citometría de flujo con análisis de imágenes. Se encontraron los siguientes valores (medias ± IC 95%): 6.1 ± 2.7 ml de volumen, color blanquecino; 66.7 ± 10.5% de motilidad; 262.7 ± 20.4 x 106 espermatozoides/ml de concentración espermática; 77.7 ± 9.7% de viabilidad espermática; 85.9 ± 5.1% de alto PMM; 83.6 ± 14.9% de integridad acrosomal. Estos resultados representan el primer reporte sobre las características seminales de esta raza y podrían ser tomados como valores de referencia para una mejor selección de machos reproductores y para el diagnóstico de alteraciones reproductivas de la raza.Universidad Nacional Mayor de San Marcos, Facultad de Medicina Veterinaria2020-12-21info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://revistasinvestigacion.unmsm.edu.pe/index.php/veterinaria/article/view/1925110.15381/rivep.v31i4.19251Revista de Investigaciones Veterinarias del Perú; Vol. 31 Núm. 4 (2020); e19251Revista de Investigaciones Veterinarias del Perú; Vol. 31 No. 4 (2020); e192511682-34191609-9117reponame:Revistas - Universidad Nacional Mayor de San Marcosinstname:Universidad Nacional Mayor de San Marcosinstacron:UNMSMspahttps://revistasinvestigacion.unmsm.edu.pe/index.php/veterinaria/article/view/19251/16128Derechos de autor 2020 Alexei Santiani, María Pérez- Rosales, Carlos Challcohttps://creativecommons.org/licenses/by-nc-sa/4.0info:eu-repo/semantics/openAccessoai:ojs.csi.unmsm:article/192512020-12-21T14:03:31Z |
| dc.title.none.fl_str_mv |
Basic and functional characterization of the Peruvian Hairless Dog semen Caracterización básica y funcional del semen del Perro sin Pelo del Perú |
| title |
Basic and functional characterization of the Peruvian Hairless Dog semen |
| spellingShingle |
Basic and functional characterization of the Peruvian Hairless Dog semen Santiani, Alexei Peruvian hairless dog semen spermatozoa flow cytometry Perro sin pelo del Perú semen espermatozoide citometría de flujo |
| title_short |
Basic and functional characterization of the Peruvian Hairless Dog semen |
| title_full |
Basic and functional characterization of the Peruvian Hairless Dog semen |
| title_fullStr |
Basic and functional characterization of the Peruvian Hairless Dog semen |
| title_full_unstemmed |
Basic and functional characterization of the Peruvian Hairless Dog semen |
| title_sort |
Basic and functional characterization of the Peruvian Hairless Dog semen |
| dc.creator.none.fl_str_mv |
Santiani, Alexei Pérez- Rosales, María Challco, Carlos |
| author |
Santiani, Alexei |
| author_facet |
Santiani, Alexei Pérez- Rosales, María Challco, Carlos |
| author_role |
author |
| author2 |
Pérez- Rosales, María Challco, Carlos |
| author2_role |
author author |
| dc.subject.none.fl_str_mv |
Peruvian hairless dog semen spermatozoa flow cytometry Perro sin pelo del Perú semen espermatozoide citometría de flujo |
| topic |
Peruvian hairless dog semen spermatozoa flow cytometry Perro sin pelo del Perú semen espermatozoide citometría de flujo |
| description |
The aim of this study was to characterize the semen of the Peruvian Hairless Dog. Fifteen males, aged 1 to 5 years, registered by the Kennel Club Peruano were used to collect semen (one ejaculated per male) by digital manipulation. A basic spermatogram was performed and macroscopic (volume and colour) and microscopic (sperm motility and concentration, using light field microscopy) characteristics were recorded. Additionally, sperm function tests were performed: sperm viability (using SYBR-14 and propidium iodide), mitochondrial membrane potential (MMP, using MitoTraker Deep Red FM), and acrosomal integrity (using FITC-PSA and propidium iodide) and analysed by flow cytometry with image analysis. The following values were found (mean ± 95% CI): 6.1 ± 2.7 ml volume, opalescent colour; 66.7 ± 10.5% motility; 262.7 ± 20.4 x 106 sperm/ml sperm concentration; 77.7 ± 9.7% of sperm viability; 85.9 ± 5.1% high MMP; 83.6 ± 14.9% acrosomal integrity. These results represent the first report on the seminal characteristics of this breed and could be taken as reference values for a better selection of breeding males and for the diagnosis of reproductive alterations of the breed. |
| publishDate |
2020 |
| dc.date.none.fl_str_mv |
2020-12-21 |
| dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
| format |
article |
| status_str |
publishedVersion |
| dc.identifier.none.fl_str_mv |
https://revistasinvestigacion.unmsm.edu.pe/index.php/veterinaria/article/view/19251 10.15381/rivep.v31i4.19251 |
| url |
https://revistasinvestigacion.unmsm.edu.pe/index.php/veterinaria/article/view/19251 |
| identifier_str_mv |
10.15381/rivep.v31i4.19251 |
| dc.language.none.fl_str_mv |
spa |
| language |
spa |
| dc.relation.none.fl_str_mv |
https://revistasinvestigacion.unmsm.edu.pe/index.php/veterinaria/article/view/19251/16128 |
| dc.rights.none.fl_str_mv |
Derechos de autor 2020 Alexei Santiani, María Pérez- Rosales, Carlos Challco https://creativecommons.org/licenses/by-nc-sa/4.0 info:eu-repo/semantics/openAccess |
| rights_invalid_str_mv |
Derechos de autor 2020 Alexei Santiani, María Pérez- Rosales, Carlos Challco https://creativecommons.org/licenses/by-nc-sa/4.0 |
| eu_rights_str_mv |
openAccess |
| dc.format.none.fl_str_mv |
application/pdf |
| dc.publisher.none.fl_str_mv |
Universidad Nacional Mayor de San Marcos, Facultad de Medicina Veterinaria |
| publisher.none.fl_str_mv |
Universidad Nacional Mayor de San Marcos, Facultad de Medicina Veterinaria |
| dc.source.none.fl_str_mv |
Revista de Investigaciones Veterinarias del Perú; Vol. 31 Núm. 4 (2020); e19251 Revista de Investigaciones Veterinarias del Perú; Vol. 31 No. 4 (2020); e19251 1682-3419 1609-9117 reponame:Revistas - Universidad Nacional Mayor de San Marcos instname:Universidad Nacional Mayor de San Marcos instacron:UNMSM |
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Universidad Nacional Mayor de San Marcos |
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UNMSM |
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UNMSM |
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Revistas - Universidad Nacional Mayor de San Marcos |
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Revistas - Universidad Nacional Mayor de San Marcos |
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13.871978 |
Nota importante:
La información contenida en este registro es de entera responsabilidad de la institución que gestiona el repositorio institucional donde esta contenido este documento o set de datos. El CONCYTEC no se hace responsable por los contenidos (publicaciones y/o datos) accesibles a través del Repositorio Nacional Digital de Ciencia, Tecnología e Innovación de Acceso Abierto (ALICIA).
La información contenida en este registro es de entera responsabilidad de la institución que gestiona el repositorio institucional donde esta contenido este documento o set de datos. El CONCYTEC no se hace responsable por los contenidos (publicaciones y/o datos) accesibles a través del Repositorio Nacional Digital de Ciencia, Tecnología e Innovación de Acceso Abierto (ALICIA).
