Detection, quantification and morphological characterization of Vibrio cholerae indicator bacteriophages

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The specificity between bacteriophages and bacteria is a feature that has been successfully used for the detection of several microbial species. For this reason the detection of vibriophages is a valuable research tool, and it might be a fast and convenient method to Vibrio cholerae diagnostics. The...

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Detalles Bibliográficos
Autores: Talledo, Miguel, Gutiérrez, Susana, Merino, Fernando, Rojas, Nancy
Formato: artículo
Fecha de Publicación:1998
Institución:Universidad Nacional Mayor de San Marcos
Repositorio:Revistas - Universidad Nacional Mayor de San Marcos
Lenguaje:español
OAI Identifier:oai:ojs.csi.unmsm:article/8324
Enlace del recurso:https://revistasinvestigacion.unmsm.edu.pe/index.php/rpb/article/view/8324
Nivel de acceso:acceso abierto
Materia:Vibrio cholerae
vibriófagos
contaminación
aguas
bacteriófagos.
vibriophages
pollution
water
bacteriophages.
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spelling Detection, quantification and morphological characterization of Vibrio cholerae indicator bacteriophagesDetección, cuantificación y caracterización morfológica de bacteriófagos indicadores de Vibrio CholeraeTalledo, MiguelGutiérrez, SusanaMerino, FernandoRojas, NancyVibrio choleraevibriófagoscontaminaciónaguasbacteriófagos.Vibrio choleraevibriophagespollutionwaterbacteriophages.The specificity between bacteriophages and bacteria is a feature that has been successfully used for the detection of several microbial species. For this reason the detection of vibriophages is a valuable research tool, and it might be a fast and convenient method to Vibrio cholerae diagnostics. The goal of this study was to detect vibriophages in shallow seawater samples and to determine the morphological characteristics of these vibriophages. It was determined the growth kinetics of a strain of Vibrio cholerae serotype Inaba. Seawater samples taken from five shallow points from an adjacent sector to La Chira beach, and Rímac and Chillón river mouths were analyzed qualitatively and quantitatively using different inoculum volumes and several incubation periods. The bacteriophages were concentrated and stained for the morphological study by transmission electron microscopy. The results we obtained indicate that the vibriophages could be an important tool as a Vibrio cholerae indicator.La especificidad entre bacteriófagos y bacterias es una característica utilizada exitosamente para la detección de varias especies microbianas, Por este motivo, la detección de vibriófagos es una herramienta útil de investigación y podría ser un método rápido y conveniente de diagnóstico de Vibrio cholerae. El objetivo de este estudio fue detectar vibriófagos en muestras de aguas marinas someras y determinar las características morfológicas de estos vibriófagos. Se determinó la cinética de crecimiento de una cepa de Vibrio cholerae serotipo Inaba. Se analizaron cualitativa y cuantitativamente muestras tomadas de cinco puntos de un sector adyacente a la playa La Chira y de las desembocaduras del río Rímac y río Chillón, usándose distintos inóculos y varios periodos de incubación. Los bacteriófagos fueron concentrados y teñidos para el estudio morfológico por microscopía electrónica de transmisión. Los resultados obtenidos indican que la detección de vibriófagos podría ser una herramienta importante como indicador de la presencia de Vibrio cholerae.Universidad Nacional Mayor de San Marcos, Facultad de Ciencias Biológicas1998-12-31info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://revistasinvestigacion.unmsm.edu.pe/index.php/rpb/article/view/832410.15381/rpb.v5i2.8324Revista Peruana de Biología; Vol. 5 Núm. 2 (1998); 090 - 097Revista Peruana de Biología; Vol. 5 No. 2 (1998); 090 - 0971727-99331561-0837reponame:Revistas - Universidad Nacional Mayor de San Marcosinstname:Universidad Nacional Mayor de San Marcosinstacron:UNMSMspahttps://revistasinvestigacion.unmsm.edu.pe/index.php/rpb/article/view/8324/7249Derechos de autor 1998 Miguel Talledo, Susana Gutiérrez, Fernando Merino, Nancy Rojashttps://creativecommons.org/licenses/by-nc-sa/4.0info:eu-repo/semantics/openAccessoai:ojs.csi.unmsm:article/83242020-05-26T15:25:28Z
dc.title.none.fl_str_mv Detection, quantification and morphological characterization of Vibrio cholerae indicator bacteriophages
Detección, cuantificación y caracterización morfológica de bacteriófagos indicadores de Vibrio Cholerae
title Detection, quantification and morphological characterization of Vibrio cholerae indicator bacteriophages
spellingShingle Detection, quantification and morphological characterization of Vibrio cholerae indicator bacteriophages
Talledo, Miguel
Vibrio cholerae
vibriófagos
contaminación
aguas
bacteriófagos.
Vibrio cholerae
vibriophages
pollution
water
bacteriophages.
title_short Detection, quantification and morphological characterization of Vibrio cholerae indicator bacteriophages
title_full Detection, quantification and morphological characterization of Vibrio cholerae indicator bacteriophages
title_fullStr Detection, quantification and morphological characterization of Vibrio cholerae indicator bacteriophages
title_full_unstemmed Detection, quantification and morphological characterization of Vibrio cholerae indicator bacteriophages
title_sort Detection, quantification and morphological characterization of Vibrio cholerae indicator bacteriophages
dc.creator.none.fl_str_mv Talledo, Miguel
Gutiérrez, Susana
Merino, Fernando
Rojas, Nancy
author Talledo, Miguel
author_facet Talledo, Miguel
Gutiérrez, Susana
Merino, Fernando
Rojas, Nancy
author_role author
author2 Gutiérrez, Susana
Merino, Fernando
Rojas, Nancy
author2_role author
author
author
dc.subject.none.fl_str_mv Vibrio cholerae
vibriófagos
contaminación
aguas
bacteriófagos.
Vibrio cholerae
vibriophages
pollution
water
bacteriophages.
topic Vibrio cholerae
vibriófagos
contaminación
aguas
bacteriófagos.
Vibrio cholerae
vibriophages
pollution
water
bacteriophages.
description The specificity between bacteriophages and bacteria is a feature that has been successfully used for the detection of several microbial species. For this reason the detection of vibriophages is a valuable research tool, and it might be a fast and convenient method to Vibrio cholerae diagnostics. The goal of this study was to detect vibriophages in shallow seawater samples and to determine the morphological characteristics of these vibriophages. It was determined the growth kinetics of a strain of Vibrio cholerae serotype Inaba. Seawater samples taken from five shallow points from an adjacent sector to La Chira beach, and Rímac and Chillón river mouths were analyzed qualitatively and quantitatively using different inoculum volumes and several incubation periods. The bacteriophages were concentrated and stained for the morphological study by transmission electron microscopy. The results we obtained indicate that the vibriophages could be an important tool as a Vibrio cholerae indicator.
publishDate 1998
dc.date.none.fl_str_mv 1998-12-31
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://revistasinvestigacion.unmsm.edu.pe/index.php/rpb/article/view/8324
10.15381/rpb.v5i2.8324
url https://revistasinvestigacion.unmsm.edu.pe/index.php/rpb/article/view/8324
identifier_str_mv 10.15381/rpb.v5i2.8324
dc.language.none.fl_str_mv spa
language spa
dc.relation.none.fl_str_mv https://revistasinvestigacion.unmsm.edu.pe/index.php/rpb/article/view/8324/7249
dc.rights.none.fl_str_mv Derechos de autor 1998 Miguel Talledo, Susana Gutiérrez, Fernando Merino, Nancy Rojas
https://creativecommons.org/licenses/by-nc-sa/4.0
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Derechos de autor 1998 Miguel Talledo, Susana Gutiérrez, Fernando Merino, Nancy Rojas
https://creativecommons.org/licenses/by-nc-sa/4.0
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Universidad Nacional Mayor de San Marcos, Facultad de Ciencias Biológicas
publisher.none.fl_str_mv Universidad Nacional Mayor de San Marcos, Facultad de Ciencias Biológicas
dc.source.none.fl_str_mv Revista Peruana de Biología; Vol. 5 Núm. 2 (1998); 090 - 097
Revista Peruana de Biología; Vol. 5 No. 2 (1998); 090 - 097
1727-9933
1561-0837
reponame:Revistas - Universidad Nacional Mayor de San Marcos
instname:Universidad Nacional Mayor de San Marcos
instacron:UNMSM
instname_str Universidad Nacional Mayor de San Marcos
instacron_str UNMSM
institution UNMSM
reponame_str Revistas - Universidad Nacional Mayor de San Marcos
collection Revistas - Universidad Nacional Mayor de San Marcos
repository.name.fl_str_mv
repository.mail.fl_str_mv
_version_ 1795238310690422784
score 13.962735
Detection, quantification and morphological characterization of Vibrio cholerae indicator bacteriophages
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