A serological and molecular survey of Ehrlichia canis in dogs from a community in Aragua State, Venezuela
Descripción del Articulo
The aims of the study were to determine the prevalence of antibodies anti-E canis and the molecular detection of E. canis as well as to determine the species and level of tick infestations in dogs from a rural community in Aragua state, Venezuela. Blood samples from 110 domestic dogs were obtained a...
| Autores: | , , , , |
|---|---|
| Formato: | artículo |
| Fecha de Publicación: | 2015 |
| Institución: | Universidad Nacional Mayor de San Marcos |
| Repositorio: | Revistas - Universidad Nacional Mayor de San Marcos |
| Lenguaje: | español |
| OAI Identifier: | oai:ojs.csi.unmsm:article/11220 |
| Enlace del recurso: | https://revistasinvestigacion.unmsm.edu.pe/index.php/veterinaria/article/view/11220 |
| Nivel de acceso: | acceso abierto |
| Materia: | Ehrlichia canis ehrlichiosis IFI PCR Rhipicephalus sanguineus Venezuela Riphicephalus sanguineus |
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A serological and molecular survey of Ehrlichia canis in dogs from a community in Aragua State, VenezuelaEstudio Serológico y Molecular de Ehrlichia canis en Perros de una Comunidad del Estado Aragua, VenezuelaMartínez A., María del CarmenArraga-Alvarado, Cruz MaríaTriana-Alonso, Francisco JavierRuiz C., Johanny AlejandraGutiérrez G., Clara NancyEhrlichia canisehrlichiosisIFIPCRRhipicephalus sanguineusVenezuelaEhrlichia canisehrlichiosisIFIPCRRiphicephalus sanguineusVenezuelaThe aims of the study were to determine the prevalence of antibodies anti-E canis and the molecular detection of E. canis as well as to determine the species and level of tick infestations in dogs from a rural community in Aragua state, Venezuela. Blood samples from 110 domestic dogs were obtained and analyzed by Indirect Immunofluorescence (IFI) and Nested Polymerase Chain Reaction (PCR). The seroprevalence was 77.3% and the molecular detection was 45.2%. The percentage of dogs infested with ticks was 69%, mostly with Rhipicephalus sanguineus. The results showed that 80% (68/85) of seropositive dogs and 32% (8/25) of seronegative dogs were tick infested (OR=8.5, 95%, CI 3.19 to 22.6; p<0.00001).Los objetivos de esta investigación fueron determinar la prevalencia de anticuerpos anti-E. canis y la detección molecular de E. canis, así como determinar el nivel de infestación y especies de garrapatas en perros de una comunidad rural del estado Aragua, Venezuela. Se recolectaron muestras sanguíneas de 110 perros domésticos y fueron evaluadas por inmunofluorescencia indirecta (IFI) y por la técnica de reacción en cadena de la polimerasa anidada (PCR). La seroprevalencia resultó en 77.3% y la detección molecular fue de 45.2%. El porcentaje de perros infestados por garrapatas fue de 69%, identificándose mayormente a Riphicephalus sanguineus. Se determinó que 80% (68/85) de los perros seropositivos y el 32% (8/25) de los seronegativos estaban infestados por garrapatas (OR=8.5, IC 95%: 3.19-22.6; p<0.00001).Universidad Nacional Mayor de San Marcos, Facultad de Medicina Veterinaria2015-12-31info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://revistasinvestigacion.unmsm.edu.pe/index.php/veterinaria/article/view/1122010.15381/rivep.v26i4.11220Revista de Investigaciones Veterinarias del Perú; Vol. 26 Núm. 4 (2015); 648-656Revista de Investigaciones Veterinarias del Perú; Vol. 26 No. 4 (2015); 648-6561682-34191609-9117reponame:Revistas - Universidad Nacional Mayor de San Marcosinstname:Universidad Nacional Mayor de San Marcosinstacron:UNMSMspahttps://revistasinvestigacion.unmsm.edu.pe/index.php/veterinaria/article/view/11220/10290Derechos de autor 2015 María del Carmen Martínez A., Cruz María Arraga-Alvarado, Francisco Javier Triana-Alonso, Johanny Alejandra Ruiz C., Clara Nancy Gutiérrez G.https://creativecommons.org/licenses/by-nc-sa/4.0info:eu-repo/semantics/openAccessoai:ojs.csi.unmsm:article/112202020-03-29T20:10:42Z |
| dc.title.none.fl_str_mv |
A serological and molecular survey of Ehrlichia canis in dogs from a community in Aragua State, Venezuela Estudio Serológico y Molecular de Ehrlichia canis en Perros de una Comunidad del Estado Aragua, Venezuela |
| title |
A serological and molecular survey of Ehrlichia canis in dogs from a community in Aragua State, Venezuela |
| spellingShingle |
A serological and molecular survey of Ehrlichia canis in dogs from a community in Aragua State, Venezuela Martínez A., María del Carmen Ehrlichia canis ehrlichiosis IFI PCR Rhipicephalus sanguineus Venezuela Ehrlichia canis ehrlichiosis IFI PCR Riphicephalus sanguineus Venezuela |
| title_short |
A serological and molecular survey of Ehrlichia canis in dogs from a community in Aragua State, Venezuela |
| title_full |
A serological and molecular survey of Ehrlichia canis in dogs from a community in Aragua State, Venezuela |
| title_fullStr |
A serological and molecular survey of Ehrlichia canis in dogs from a community in Aragua State, Venezuela |
| title_full_unstemmed |
A serological and molecular survey of Ehrlichia canis in dogs from a community in Aragua State, Venezuela |
| title_sort |
A serological and molecular survey of Ehrlichia canis in dogs from a community in Aragua State, Venezuela |
| dc.creator.none.fl_str_mv |
Martínez A., María del Carmen Arraga-Alvarado, Cruz María Triana-Alonso, Francisco Javier Ruiz C., Johanny Alejandra Gutiérrez G., Clara Nancy |
| author |
Martínez A., María del Carmen |
| author_facet |
Martínez A., María del Carmen Arraga-Alvarado, Cruz María Triana-Alonso, Francisco Javier Ruiz C., Johanny Alejandra Gutiérrez G., Clara Nancy |
| author_role |
author |
| author2 |
Arraga-Alvarado, Cruz María Triana-Alonso, Francisco Javier Ruiz C., Johanny Alejandra Gutiérrez G., Clara Nancy |
| author2_role |
author author author author |
| dc.subject.none.fl_str_mv |
Ehrlichia canis ehrlichiosis IFI PCR Rhipicephalus sanguineus Venezuela Ehrlichia canis ehrlichiosis IFI PCR Riphicephalus sanguineus Venezuela |
| topic |
Ehrlichia canis ehrlichiosis IFI PCR Rhipicephalus sanguineus Venezuela Ehrlichia canis ehrlichiosis IFI PCR Riphicephalus sanguineus Venezuela |
| description |
The aims of the study were to determine the prevalence of antibodies anti-E canis and the molecular detection of E. canis as well as to determine the species and level of tick infestations in dogs from a rural community in Aragua state, Venezuela. Blood samples from 110 domestic dogs were obtained and analyzed by Indirect Immunofluorescence (IFI) and Nested Polymerase Chain Reaction (PCR). The seroprevalence was 77.3% and the molecular detection was 45.2%. The percentage of dogs infested with ticks was 69%, mostly with Rhipicephalus sanguineus. The results showed that 80% (68/85) of seropositive dogs and 32% (8/25) of seronegative dogs were tick infested (OR=8.5, 95%, CI 3.19 to 22.6; p<0.00001). |
| publishDate |
2015 |
| dc.date.none.fl_str_mv |
2015-12-31 |
| dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
| format |
article |
| status_str |
publishedVersion |
| dc.identifier.none.fl_str_mv |
https://revistasinvestigacion.unmsm.edu.pe/index.php/veterinaria/article/view/11220 10.15381/rivep.v26i4.11220 |
| url |
https://revistasinvestigacion.unmsm.edu.pe/index.php/veterinaria/article/view/11220 |
| identifier_str_mv |
10.15381/rivep.v26i4.11220 |
| dc.language.none.fl_str_mv |
spa |
| language |
spa |
| dc.relation.none.fl_str_mv |
https://revistasinvestigacion.unmsm.edu.pe/index.php/veterinaria/article/view/11220/10290 |
| dc.rights.none.fl_str_mv |
https://creativecommons.org/licenses/by-nc-sa/4.0 info:eu-repo/semantics/openAccess |
| rights_invalid_str_mv |
https://creativecommons.org/licenses/by-nc-sa/4.0 |
| eu_rights_str_mv |
openAccess |
| dc.format.none.fl_str_mv |
application/pdf |
| dc.publisher.none.fl_str_mv |
Universidad Nacional Mayor de San Marcos, Facultad de Medicina Veterinaria |
| publisher.none.fl_str_mv |
Universidad Nacional Mayor de San Marcos, Facultad de Medicina Veterinaria |
| dc.source.none.fl_str_mv |
Revista de Investigaciones Veterinarias del Perú; Vol. 26 Núm. 4 (2015); 648-656 Revista de Investigaciones Veterinarias del Perú; Vol. 26 No. 4 (2015); 648-656 1682-3419 1609-9117 reponame:Revistas - Universidad Nacional Mayor de San Marcos instname:Universidad Nacional Mayor de San Marcos instacron:UNMSM |
| instname_str |
Universidad Nacional Mayor de San Marcos |
| instacron_str |
UNMSM |
| institution |
UNMSM |
| reponame_str |
Revistas - Universidad Nacional Mayor de San Marcos |
| collection |
Revistas - Universidad Nacional Mayor de San Marcos |
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13.888049 |
Nota importante:
La información contenida en este registro es de entera responsabilidad de la institución que gestiona el repositorio institucional donde esta contenido este documento o set de datos. El CONCYTEC no se hace responsable por los contenidos (publicaciones y/o datos) accesibles a través del Repositorio Nacional Digital de Ciencia, Tecnología e Innovación de Acceso Abierto (ALICIA).
La información contenida en este registro es de entera responsabilidad de la institución que gestiona el repositorio institucional donde esta contenido este documento o set de datos. El CONCYTEC no se hace responsable por los contenidos (publicaciones y/o datos) accesibles a través del Repositorio Nacional Digital de Ciencia, Tecnología e Innovación de Acceso Abierto (ALICIA).
