Antimicrobial resistance and genotyping of Salmonella Typhimurium strains isolated from guinea pigs (Cavia porcellus) from intensive production farms of the city of Lima, Peru

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The aim of this study was to characterize 20 strains of Salmonella enterica at molecular level and antimicrobial resistance. Of these, 15 strains were obtained from infected guinea pigs and five from clinically healthy guinea pigs from two intensive production centers located in Lima, Peru. The invA...

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Detalles Bibliográficos
Autores: Salvatierra R., Guillermo, Rimac B., Rocío, Chero O., Ana, Reyna W., Iván, Rosadio A., Raúl, Maturrano H., Lenin
Formato: artículo
Fecha de Publicación:2018
Institución:Universidad Nacional Mayor de San Marcos
Repositorio:Revistas - Universidad Nacional Mayor de San Marcos
Lenguaje:español
OAI Identifier:oai:ojs.csi.unmsm:article/14089
Enlace del recurso:https://revistasinvestigacion.unmsm.edu.pe/index.php/veterinaria/article/view/14089
Nivel de acceso:acceso abierto
Materia:salmonellosis
guinea pigs
Typhimurium
BOX-PCR
antimicrobial resistance
salmonelosis
cuyes
resistencia antimicrobiana
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oai_identifier_str oai:ojs.csi.unmsm:article/14089
network_acronym_str REVUNMSM
network_name_str Revistas - Universidad Nacional Mayor de San Marcos
repository_id_str
dc.title.none.fl_str_mv Antimicrobial resistance and genotyping of Salmonella Typhimurium strains isolated from guinea pigs (Cavia porcellus) from intensive production farms of the city of Lima, Peru
Resistencia antimicrobiana y genotipificación de cepas de Salmonella Typhimurium aisladas de cuyes (Cavia porcellus) provenientes de granjas de producción intensiva de la ciudad de Lima, Perú
title Antimicrobial resistance and genotyping of Salmonella Typhimurium strains isolated from guinea pigs (Cavia porcellus) from intensive production farms of the city of Lima, Peru
spellingShingle Antimicrobial resistance and genotyping of Salmonella Typhimurium strains isolated from guinea pigs (Cavia porcellus) from intensive production farms of the city of Lima, Peru
Salvatierra R., Guillermo
salmonellosis
guinea pigs
Typhimurium
BOX-PCR
antimicrobial resistance
salmonelosis
cuyes
Typhimurium
BOX-PCR
resistencia antimicrobiana
title_short Antimicrobial resistance and genotyping of Salmonella Typhimurium strains isolated from guinea pigs (Cavia porcellus) from intensive production farms of the city of Lima, Peru
title_full Antimicrobial resistance and genotyping of Salmonella Typhimurium strains isolated from guinea pigs (Cavia porcellus) from intensive production farms of the city of Lima, Peru
title_fullStr Antimicrobial resistance and genotyping of Salmonella Typhimurium strains isolated from guinea pigs (Cavia porcellus) from intensive production farms of the city of Lima, Peru
title_full_unstemmed Antimicrobial resistance and genotyping of Salmonella Typhimurium strains isolated from guinea pigs (Cavia porcellus) from intensive production farms of the city of Lima, Peru
title_sort Antimicrobial resistance and genotyping of Salmonella Typhimurium strains isolated from guinea pigs (Cavia porcellus) from intensive production farms of the city of Lima, Peru
dc.creator.none.fl_str_mv Salvatierra R., Guillermo
Rimac B., Rocío
Chero O., Ana
Reyna W., Iván
Rosadio A., Raúl
Maturrano H., Lenin
author Salvatierra R., Guillermo
author_facet Salvatierra R., Guillermo
Rimac B., Rocío
Chero O., Ana
Reyna W., Iván
Rosadio A., Raúl
Maturrano H., Lenin
author_role author
author2 Rimac B., Rocío
Chero O., Ana
Reyna W., Iván
Rosadio A., Raúl
Maturrano H., Lenin
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv salmonellosis
guinea pigs
Typhimurium
BOX-PCR
antimicrobial resistance
salmonelosis
cuyes
Typhimurium
BOX-PCR
resistencia antimicrobiana
topic salmonellosis
guinea pigs
Typhimurium
BOX-PCR
antimicrobial resistance
salmonelosis
cuyes
Typhimurium
BOX-PCR
resistencia antimicrobiana
description The aim of this study was to characterize 20 strains of Salmonella enterica at molecular level and antimicrobial resistance. Of these, 15 strains were obtained from infected guinea pigs and five from clinically healthy guinea pigs from two intensive production centers located in Lima, Peru. The invA, prot6E and fliC genes corresponding to the genus Salmonella and serovars Enteritidis and Typhimurium, respectively, were detected by a multiple PCR technique. Genetic variability was detected using the BOX-PCR technique using the first BOXA1R. Resistance was evaluated using the Kirby Bauer technique based on erythromycin, nitrofurantoin, streptomycin, penicillin, enrofloxacin, fosfomycin, amoxicillin with clavulanic acid, sulfatrimetoprim and ciprofloxacin. Serotype Typhimurium was determined in 100% of the isolates. The evaluation of the electrophoretic profiles obtained by the BOX-PCR technique demonstrated high homogeneity, with similar DNA bands patterns. Strains resistant to erythromycin 60% (12/20), nitrofurantoin 40% (8/20), streptomycin 30% (6/20), penicillin 25% (5/20), and enrofloxacin 10% (2/20) were detected. The detection of resistant strains may cause problems in the treatment of salmonellosis in guinea pigs and the presence of only a genetic group suggests a clonal dispersion.
publishDate 2018
dc.date.none.fl_str_mv 2018-03-14
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://revistasinvestigacion.unmsm.edu.pe/index.php/veterinaria/article/view/14089
10.15381/rivep.v29i1.14089
url https://revistasinvestigacion.unmsm.edu.pe/index.php/veterinaria/article/view/14089
identifier_str_mv 10.15381/rivep.v29i1.14089
dc.language.none.fl_str_mv spa
language spa
dc.relation.none.fl_str_mv https://revistasinvestigacion.unmsm.edu.pe/index.php/veterinaria/article/view/14089/12766
https://revistasinvestigacion.unmsm.edu.pe/index.php/veterinaria/article/view/14089/14006
dc.rights.none.fl_str_mv https://creativecommons.org/licenses/by-nc-sa/4.0
info:eu-repo/semantics/openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/4.0
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
text/html
dc.publisher.none.fl_str_mv Universidad Nacional Mayor de San Marcos, Facultad de Medicina Veterinaria
publisher.none.fl_str_mv Universidad Nacional Mayor de San Marcos, Facultad de Medicina Veterinaria
dc.source.none.fl_str_mv Revista de Investigaciones Veterinarias del Perú; Vol. 29 Núm. 1 (2018); 319-327
Revista de Investigaciones Veterinarias del Perú; Vol. 29 No. 1 (2018); 319-327
1682-3419
1609-9117
reponame:Revistas - Universidad Nacional Mayor de San Marcos
instname:Universidad Nacional Mayor de San Marcos
instacron:UNMSM
instname_str Universidad Nacional Mayor de San Marcos
instacron_str UNMSM
institution UNMSM
reponame_str Revistas - Universidad Nacional Mayor de San Marcos
collection Revistas - Universidad Nacional Mayor de San Marcos
repository.name.fl_str_mv
repository.mail.fl_str_mv
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spelling Antimicrobial resistance and genotyping of Salmonella Typhimurium strains isolated from guinea pigs (Cavia porcellus) from intensive production farms of the city of Lima, PeruResistencia antimicrobiana y genotipificación de cepas de Salmonella Typhimurium aisladas de cuyes (Cavia porcellus) provenientes de granjas de producción intensiva de la ciudad de Lima, PerúSalvatierra R., GuillermoRimac B., RocíoChero O., AnaReyna W., IvánRosadio A., RaúlMaturrano H., Leninsalmonellosisguinea pigsTyphimuriumBOX-PCRantimicrobial resistancesalmonelosiscuyesTyphimuriumBOX-PCRresistencia antimicrobianaThe aim of this study was to characterize 20 strains of Salmonella enterica at molecular level and antimicrobial resistance. Of these, 15 strains were obtained from infected guinea pigs and five from clinically healthy guinea pigs from two intensive production centers located in Lima, Peru. The invA, prot6E and fliC genes corresponding to the genus Salmonella and serovars Enteritidis and Typhimurium, respectively, were detected by a multiple PCR technique. Genetic variability was detected using the BOX-PCR technique using the first BOXA1R. Resistance was evaluated using the Kirby Bauer technique based on erythromycin, nitrofurantoin, streptomycin, penicillin, enrofloxacin, fosfomycin, amoxicillin with clavulanic acid, sulfatrimetoprim and ciprofloxacin. Serotype Typhimurium was determined in 100% of the isolates. The evaluation of the electrophoretic profiles obtained by the BOX-PCR technique demonstrated high homogeneity, with similar DNA bands patterns. Strains resistant to erythromycin 60% (12/20), nitrofurantoin 40% (8/20), streptomycin 30% (6/20), penicillin 25% (5/20), and enrofloxacin 10% (2/20) were detected. The detection of resistant strains may cause problems in the treatment of salmonellosis in guinea pigs and the presence of only a genetic group suggests a clonal dispersion.El objetivo del estudio fue caracterizar 20 cepas de Salmonella enterica a nivel molecular y de resistencia antimicrobiana. De estas, 15 fueron obtenidas de cuyes infectados y cinco de cuyes clínicamente sanos, procedentes de dos centros de producción intensiva ubicados en Lima, Perú. Mediante una técnica de PCR múltiple se detectaron los genes invA, prot6E y fliC, correspondientes al género Salmonella y serovares Enteritidis y Typhimurium, respectivamente. Se detectó la variabilidad genética mediante la técnica BOX-PCR utilizando el primer BOXA1R. La resistencia fue evaluada utilizando la técnica de Kirby Bauer en base a eritromicina, nitrofurantoína, estreptomicina, penicilina, enrofloxacina, fosfomicina, amoxicilina con ácido clavulánico, sulfatrimetoprim y ciprofloxacina. Se determinó la serovariedad Typhimurium en el 100% de los aislados. La evaluación de los perfiles electroforéticos obtenidos por la técnica de BOX-PCR demostró alta homogeneidad, con patrones de bandas de ADN similares. Se detectaron cepas resistentes a eritromicina 60% (12/20), nitrofurantoína 40% (8/20), estreptomicina 30% (6/ 20), penicilina 25% (5/20), y enrofloxacina 10% (2/20). La detección de cepas resistentes puede ocasionar problemas en el tratamiento de salmonelosis en cuyes y la presencia de un solo grupo genético sugiere una dispersión clonal.Universidad Nacional Mayor de San Marcos, Facultad de Medicina Veterinaria2018-03-14info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdftext/htmlhttps://revistasinvestigacion.unmsm.edu.pe/index.php/veterinaria/article/view/1408910.15381/rivep.v29i1.14089Revista de Investigaciones Veterinarias del Perú; Vol. 29 Núm. 1 (2018); 319-327Revista de Investigaciones Veterinarias del Perú; Vol. 29 No. 1 (2018); 319-3271682-34191609-9117reponame:Revistas - Universidad Nacional Mayor de San Marcosinstname:Universidad Nacional Mayor de San Marcosinstacron:UNMSMspahttps://revistasinvestigacion.unmsm.edu.pe/index.php/veterinaria/article/view/14089/12766https://revistasinvestigacion.unmsm.edu.pe/index.php/veterinaria/article/view/14089/14006Derechos de autor 2018 Guillermo Salvatierra R., Rocío Rimac B., Ana Chero O., Iván Reyna W., Raúl Rosadio A., Lenin Maturrano H.https://creativecommons.org/licenses/by-nc-sa/4.0info:eu-repo/semantics/openAccessoai:ojs.csi.unmsm:article/140892018-03-15T04:44:35Z
score 13.92687
Antimicrobial resistance and genotyping of Salmonella Typhimurium strains isolated from guinea pigs (Cavia porcellus) from intensive production farms of the city of Lima, Peru
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