In vitro evaluation of the antileishmanial activity of methanolic extract of seven medicinal plants
Descripción del Articulo
The purpose of this study was to determine the in vitro antileishmanial activity of the methanol extract of seven medicinal plants, and the effect on nitric oxide production in macrophages. The extracts in concentration of 250 μg/mL were tested against intracellular amastigotes. Mouse peritoneal mac...
| Autores: | , , |
|---|---|
| Formato: | artículo |
| Fecha de Publicación: | 2012 |
| Institución: | Universidad Nacional Mayor de San Marcos |
| Repositorio: | Revistas - Universidad Nacional Mayor de San Marcos |
| Lenguaje: | español |
| OAI Identifier: | oai:ojs.csi.unmsm:article/2664 |
| Enlace del recurso: | https://revistasinvestigacion.unmsm.edu.pe/index.php/farma/article/view/2664 |
| Nivel de acceso: | acceso abierto |
| Materia: | Plantas medicinales extracto metanólico Leishmania amastigotes óxido nítrico Piper aduncum Eucalyptus globulus Punica granatum. Medicinal plants methanol extract leishmaniasis nitric oxide |
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Revistas - Universidad Nacional Mayor de San Marcos |
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| dc.title.none.fl_str_mv |
In vitro evaluation of the antileishmanial activity of methanolic extract of seven medicinal plants Evaluación in vitro de la actividad antileishmaniásica del extracto metanólico de siete plantas medicinales |
| title |
In vitro evaluation of the antileishmanial activity of methanolic extract of seven medicinal plants |
| spellingShingle |
In vitro evaluation of the antileishmanial activity of methanolic extract of seven medicinal plants Rojas, Juan P Plantas medicinales extracto metanólico Leishmania amastigotes óxido nítrico Piper aduncum Eucalyptus globulus Punica granatum. Medicinal plants methanol extract leishmaniasis amastigotes nitric oxide Piper aduncum Eucalyptus globulus Punica granatum. |
| title_short |
In vitro evaluation of the antileishmanial activity of methanolic extract of seven medicinal plants |
| title_full |
In vitro evaluation of the antileishmanial activity of methanolic extract of seven medicinal plants |
| title_fullStr |
In vitro evaluation of the antileishmanial activity of methanolic extract of seven medicinal plants |
| title_full_unstemmed |
In vitro evaluation of the antileishmanial activity of methanolic extract of seven medicinal plants |
| title_sort |
In vitro evaluation of the antileishmanial activity of methanolic extract of seven medicinal plants |
| dc.creator.none.fl_str_mv |
Rojas, Juan P Ronceros, Sergio G Palacios, Olga |
| author |
Rojas, Juan P |
| author_facet |
Rojas, Juan P Ronceros, Sergio G Palacios, Olga |
| author_role |
author |
| author2 |
Ronceros, Sergio G Palacios, Olga |
| author2_role |
author author |
| dc.subject.none.fl_str_mv |
Plantas medicinales extracto metanólico Leishmania amastigotes óxido nítrico Piper aduncum Eucalyptus globulus Punica granatum. Medicinal plants methanol extract leishmaniasis amastigotes nitric oxide Piper aduncum Eucalyptus globulus Punica granatum. |
| topic |
Plantas medicinales extracto metanólico Leishmania amastigotes óxido nítrico Piper aduncum Eucalyptus globulus Punica granatum. Medicinal plants methanol extract leishmaniasis amastigotes nitric oxide Piper aduncum Eucalyptus globulus Punica granatum. |
| description |
The purpose of this study was to determine the in vitro antileishmanial activity of the methanol extract of seven medicinal plants, and the effect on nitric oxide production in macrophages. The extracts in concentration of 250 μg/mL were tested against intracellular amastigotes. Mouse peritoneal macrophages infected with Leishmania peruviana were stained with Giemsa and examined microscopically to determine the parasite load by 100 macrophages. Nitric oxide was determined indirectly by its conversion to nitrite. Piper aduncum (matico) reduced the survival of amastigotes to 51,30% (p < 0,001), also observed a slight effect of Eucalyptus globulus (eucalyptus) and Punica granatum (pomegranate), while Annona cherimola (custard), Annona muricata (graviola) and Origanum vulgare (oregano) were inactive. Nitric oxide production by macrophages was increased significantly by the effect of Annona cherimola, which led to concentration of 13,50 ± 1,50 μM compared with 4,90 ± 0,10 μM in the control (p < 0,001). It also produced significant increase of nitrite with Origanum vulgare (p < 0,05) and Punica granatum (p < 0,01). Was conclude that Piper aduncum, Eucalyptus globulus and Punica granatum showed moderate antileishmanial effect in vitro, and the production of nitric oxide is one of the possible mechanisms of action for Punica granatum. |
| publishDate |
2012 |
| dc.date.none.fl_str_mv |
2012-12-31 |
| dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
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article |
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publishedVersion |
| dc.identifier.none.fl_str_mv |
https://revistasinvestigacion.unmsm.edu.pe/index.php/farma/article/view/2664 10.15381/ci.v15i2.2664 |
| url |
https://revistasinvestigacion.unmsm.edu.pe/index.php/farma/article/view/2664 |
| identifier_str_mv |
10.15381/ci.v15i2.2664 |
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spa |
| language |
spa |
| dc.relation.none.fl_str_mv |
https://revistasinvestigacion.unmsm.edu.pe/index.php/farma/article/view/2664/2331 |
| dc.rights.none.fl_str_mv |
Derechos de autor 2012 Juan P Rojas, Sergio G Ronceros, Olga Palacios https://creativecommons.org/licenses/by-nc-sa/4.0 info:eu-repo/semantics/openAccess |
| rights_invalid_str_mv |
Derechos de autor 2012 Juan P Rojas, Sergio G Ronceros, Olga Palacios https://creativecommons.org/licenses/by-nc-sa/4.0 |
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openAccess |
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application/pdf |
| dc.publisher.none.fl_str_mv |
Universidad Nacional Mayor de San Marcos, Facultad de Farmacia y Bioquímica |
| publisher.none.fl_str_mv |
Universidad Nacional Mayor de San Marcos, Facultad de Farmacia y Bioquímica |
| dc.source.none.fl_str_mv |
Ciencia e Investigación; Vol. 15 Núm. 2 (2012); 90-95 Ciencia e Investigación; Vol. 15 No. 2 (2012); 90-95 1609-9044 1561-0861 reponame:Revistas - Universidad Nacional Mayor de San Marcos instname:Universidad Nacional Mayor de San Marcos instacron:UNMSM |
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Universidad Nacional Mayor de San Marcos |
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UNMSM |
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UNMSM |
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Revistas - Universidad Nacional Mayor de San Marcos |
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Revistas - Universidad Nacional Mayor de San Marcos |
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1795238295086563328 |
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In vitro evaluation of the antileishmanial activity of methanolic extract of seven medicinal plantsEvaluación in vitro de la actividad antileishmaniásica del extracto metanólico de siete plantas medicinalesRojas, Juan PRonceros, Sergio GPalacios, OlgaPlantas medicinalesextracto metanólicoLeishmaniaamastigotesóxido nítricoPiper aduncumEucalyptus globulusPunica granatum.Medicinal plantsmethanol extractleishmaniasisamastigotesnitric oxidePiper aduncumEucalyptus globulusPunica granatum.The purpose of this study was to determine the in vitro antileishmanial activity of the methanol extract of seven medicinal plants, and the effect on nitric oxide production in macrophages. The extracts in concentration of 250 μg/mL were tested against intracellular amastigotes. Mouse peritoneal macrophages infected with Leishmania peruviana were stained with Giemsa and examined microscopically to determine the parasite load by 100 macrophages. Nitric oxide was determined indirectly by its conversion to nitrite. Piper aduncum (matico) reduced the survival of amastigotes to 51,30% (p < 0,001), also observed a slight effect of Eucalyptus globulus (eucalyptus) and Punica granatum (pomegranate), while Annona cherimola (custard), Annona muricata (graviola) and Origanum vulgare (oregano) were inactive. Nitric oxide production by macrophages was increased significantly by the effect of Annona cherimola, which led to concentration of 13,50 ± 1,50 μM compared with 4,90 ± 0,10 μM in the control (p < 0,001). It also produced significant increase of nitrite with Origanum vulgare (p < 0,05) and Punica granatum (p < 0,01). Was conclude that Piper aduncum, Eucalyptus globulus and Punica granatum showed moderate antileishmanial effect in vitro, and the production of nitric oxide is one of the possible mechanisms of action for Punica granatum.El propósito del presente estudio fue determinar la actividad antileishmaniásica in vitro del extracto metanólico de 7 plantas medicinales, y su efecto sobre la producción de óxido nítrico en macrófagos. Los extractos, en concentración de 250 μg/mL, fueron probados contra amastigotes intracelulares. Los macrófagos peritoneales de ratón infectados con amastigotes de Leishmania peruviana fueron coloreados con Giemsa y examinados microscópicamente para determinar la carga parasitaria por 100 macrófagos. El óxido nítrico se determinó indirectamente por su conversión a nitrito. Piper aduncum (matico) redujo la supervivencia de los amastigotes hasta 51,30% (p < 0,001), también se observó efecto reductor con Eucalyptus globulus (eucalipto) y Punica granatum (granado); mientras que Annona cherimola (chirimoya), Annona muricata (guanábana) y Origanum vulgare (orégano) fueron inactivos. La producción de óxido nítrico por los macrófagos fue incrementada significativamente por efecto de Annona cherimola, que produjo la concentración de 13,50 ± 1,50 μM en comparación con 4,90 ± 0,10 μM del control (p < 0,001). También causaron incremento significativo de nitrito Origanum vulgare (p < 0,05) y Punica granatum (p < 0,01). Se concluye que Piper aduncum, Eucalyptus globulus y Punica granatum exhiben moderado efecto antileishmaniásico in vitro, y que la producción de óxido nítrico es uno de los probables mecanismos de acción para Punica granatum.Universidad Nacional Mayor de San Marcos, Facultad de Farmacia y Bioquímica2012-12-31info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://revistasinvestigacion.unmsm.edu.pe/index.php/farma/article/view/266410.15381/ci.v15i2.2664Ciencia e Investigación; Vol. 15 Núm. 2 (2012); 90-95Ciencia e Investigación; Vol. 15 No. 2 (2012); 90-951609-90441561-0861reponame:Revistas - Universidad Nacional Mayor de San Marcosinstname:Universidad Nacional Mayor de San Marcosinstacron:UNMSMspahttps://revistasinvestigacion.unmsm.edu.pe/index.php/farma/article/view/2664/2331Derechos de autor 2012 Juan P Rojas, Sergio G Ronceros, Olga Palacioshttps://creativecommons.org/licenses/by-nc-sa/4.0info:eu-repo/semantics/openAccessoai:ojs.csi.unmsm:article/26642020-04-27T10:29:29Z |
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13.919782 |
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La información contenida en este registro es de entera responsabilidad de la institución que gestiona el repositorio institucional donde esta contenido este documento o set de datos. El CONCYTEC no se hace responsable por los contenidos (publicaciones y/o datos) accesibles a través del Repositorio Nacional Digital de Ciencia, Tecnología e Innovación de Acceso Abierto (ALICIA).
La información contenida en este registro es de entera responsabilidad de la institución que gestiona el repositorio institucional donde esta contenido este documento o set de datos. El CONCYTEC no se hace responsable por los contenidos (publicaciones y/o datos) accesibles a través del Repositorio Nacional Digital de Ciencia, Tecnología e Innovación de Acceso Abierto (ALICIA).
