Evaluation of a Multiplex-PCR method for rapid detection of Salmonella Typhimurium and Enteritidis in naturally infected guinea pigs (Cavia porcellus)

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The aim of this study was to evaluate the detection capacity of the Multiplex Polymerase Chain Reaction (PCR) method from non-selective pre-enrichment samples (using target sequences of InvA, fliC and prot6E genes) to diagnose Salmonella Typhimurium and Enteritidis and to determine the concordance b...

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Detalles Bibliográficos
Autores: Díaz O., Gerardo, Rosadio A., Raúl, Marcelo M., Geraldine, Chero O., Ana, Jiménez A., Ronald, Reyna W., Iván, Maturrano H., Lenin
Formato: artículo
Fecha de Publicación:2017
Institución:Universidad Nacional Mayor de San Marcos
Repositorio:Revistas - Universidad Nacional Mayor de San Marcos
Lenguaje:español
OAI Identifier:oai:ojs.csi.unmsm:article/13361
Enlace del recurso:https://revistasinvestigacion.unmsm.edu.pe/index.php/veterinaria/article/view/13361
Nivel de acceso:acceso abierto
Materia:multiplex PCR
rapid detection
microbiological isolation
Salmonella
guinea pig
PCR múltiple
detección rápida
análisis microbiológico
cobayo
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spelling Evaluation of a Multiplex-PCR method for rapid detection of Salmonella Typhimurium and Enteritidis in naturally infected guinea pigs (Cavia porcellus)Evaluación de una Técnica de PCR-Múltiple para la Detección Rápida de Salmonella Typhimurium y Enteritidis en Cuyes (Cavia porcellus) Naturalmente InfectadosDíaz O., GerardoRosadio A., RaúlMarcelo M., GeraldineChero O., AnaJiménez A., RonaldReyna W., IvánMaturrano H., Leninmultiplex PCRrapid detectionmicrobiological isolationSalmonellaguinea pigPCR múltipledetección rápidaanálisis microbiológicoSalmonellacobayoThe aim of this study was to evaluate the detection capacity of the Multiplex Polymerase Chain Reaction (PCR) method from non-selective pre-enrichment samples (using target sequences of InvA, fliC and prot6E genes) to diagnose Salmonella Typhimurium and Enteritidis and to determine the concordance between the conventional microbiological detection method which consist of non-selective pre-enrichment, selective enrichment, differential agar isolation and biochemical tests. A total of 111 liver samples from guinea pigs with presumptive diagnosis of salmonellosis, collected in Chancay (Lima) and El Mantaro (Junín), Peru were analyzed. Salmonella Typhimurium was detected by multiplex PCR in 54% (60/111) of the samples and by microbiological analysis in 41% (45/111). A substantial concordance was found with a Kappa value of 0.64. The McNemar test showed that the results of both tests were statistically different (p<0.05).El objetivo del estudio fue evaluar la capacidad de detección de Salmonella Typhimurium y Enteritidis mediante el método de la Reacción en Cadena de la Polimerasa (PCR) Múltiple a partir de muestras en pre-enriquecimiento no selectivo (utilizando secuencias blanco de los genes InvA, fliC y prot6E), y hallar su concordancia con el método de detección microbiológica convencional, que consta de pre-enriquecimiento no selectivo, enriquecimiento selectivo, aislamiento en agar diferencial y pruebas bioquímicas. Se analizaron 111 muestras de hígado de cuyes con diagnóstico presuntivo de Salmonelosis, provenientes de Chancay (Lima) y El Mantaro (Junín), Perú. Se llegó a detectar Salmonella Typhimurium por PCR Múltiple en 54% (60/111) de las muestras y en 41% (45/111) por análisis microbiológico. Se encontró una concordancia substancial con un valor de Kappa de 0.64. La prueba de McNemar demostró que los resultados de ambas pruebas son estadísticamente diferentes (p<0.05).Universidad Nacional Mayor de San Marcos, Facultad de Medicina Veterinaria2017-10-11info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://revistasinvestigacion.unmsm.edu.pe/index.php/veterinaria/article/view/1336110.15381/rivep.v28i3.13361Revista de Investigaciones Veterinarias del Perú; Vol. 28 Núm. 3 (2017); 713-722Revista de Investigaciones Veterinarias del Perú; Vol. 28 No. 3 (2017); 713-7221682-34191609-9117reponame:Revistas - Universidad Nacional Mayor de San Marcosinstname:Universidad Nacional Mayor de San Marcosinstacron:UNMSMspahttps://revistasinvestigacion.unmsm.edu.pe/index.php/veterinaria/article/view/13361/12270Derechos de autor 2017 Gerardo Díaz O., Raúl Rosadio A., Geraldine Marcelo M., Ana Chero O., Ronald Jiménez A., Iván Reyna W., Lenin Maturrano H.https://creativecommons.org/licenses/by-nc-sa/4.0info:eu-repo/semantics/openAccessoai:ojs.csi.unmsm:article/133612017-12-19T16:57:15Z
dc.title.none.fl_str_mv Evaluation of a Multiplex-PCR method for rapid detection of Salmonella Typhimurium and Enteritidis in naturally infected guinea pigs (Cavia porcellus)
Evaluación de una Técnica de PCR-Múltiple para la Detección Rápida de Salmonella Typhimurium y Enteritidis en Cuyes (Cavia porcellus) Naturalmente Infectados
title Evaluation of a Multiplex-PCR method for rapid detection of Salmonella Typhimurium and Enteritidis in naturally infected guinea pigs (Cavia porcellus)
spellingShingle Evaluation of a Multiplex-PCR method for rapid detection of Salmonella Typhimurium and Enteritidis in naturally infected guinea pigs (Cavia porcellus)
Díaz O., Gerardo
multiplex PCR
rapid detection
microbiological isolation
Salmonella
guinea pig
PCR múltiple
detección rápida
análisis microbiológico
Salmonella
cobayo
title_short Evaluation of a Multiplex-PCR method for rapid detection of Salmonella Typhimurium and Enteritidis in naturally infected guinea pigs (Cavia porcellus)
title_full Evaluation of a Multiplex-PCR method for rapid detection of Salmonella Typhimurium and Enteritidis in naturally infected guinea pigs (Cavia porcellus)
title_fullStr Evaluation of a Multiplex-PCR method for rapid detection of Salmonella Typhimurium and Enteritidis in naturally infected guinea pigs (Cavia porcellus)
title_full_unstemmed Evaluation of a Multiplex-PCR method for rapid detection of Salmonella Typhimurium and Enteritidis in naturally infected guinea pigs (Cavia porcellus)
title_sort Evaluation of a Multiplex-PCR method for rapid detection of Salmonella Typhimurium and Enteritidis in naturally infected guinea pigs (Cavia porcellus)
dc.creator.none.fl_str_mv Díaz O., Gerardo
Rosadio A., Raúl
Marcelo M., Geraldine
Chero O., Ana
Jiménez A., Ronald
Reyna W., Iván
Maturrano H., Lenin
author Díaz O., Gerardo
author_facet Díaz O., Gerardo
Rosadio A., Raúl
Marcelo M., Geraldine
Chero O., Ana
Jiménez A., Ronald
Reyna W., Iván
Maturrano H., Lenin
author_role author
author2 Rosadio A., Raúl
Marcelo M., Geraldine
Chero O., Ana
Jiménez A., Ronald
Reyna W., Iván
Maturrano H., Lenin
author2_role author
author
author
author
author
author
dc.subject.none.fl_str_mv multiplex PCR
rapid detection
microbiological isolation
Salmonella
guinea pig
PCR múltiple
detección rápida
análisis microbiológico
Salmonella
cobayo
topic multiplex PCR
rapid detection
microbiological isolation
Salmonella
guinea pig
PCR múltiple
detección rápida
análisis microbiológico
Salmonella
cobayo
description The aim of this study was to evaluate the detection capacity of the Multiplex Polymerase Chain Reaction (PCR) method from non-selective pre-enrichment samples (using target sequences of InvA, fliC and prot6E genes) to diagnose Salmonella Typhimurium and Enteritidis and to determine the concordance between the conventional microbiological detection method which consist of non-selective pre-enrichment, selective enrichment, differential agar isolation and biochemical tests. A total of 111 liver samples from guinea pigs with presumptive diagnosis of salmonellosis, collected in Chancay (Lima) and El Mantaro (Junín), Peru were analyzed. Salmonella Typhimurium was detected by multiplex PCR in 54% (60/111) of the samples and by microbiological analysis in 41% (45/111). A substantial concordance was found with a Kappa value of 0.64. The McNemar test showed that the results of both tests were statistically different (p<0.05).
publishDate 2017
dc.date.none.fl_str_mv 2017-10-11
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://revistasinvestigacion.unmsm.edu.pe/index.php/veterinaria/article/view/13361
10.15381/rivep.v28i3.13361
url https://revistasinvestigacion.unmsm.edu.pe/index.php/veterinaria/article/view/13361
identifier_str_mv 10.15381/rivep.v28i3.13361
dc.language.none.fl_str_mv spa
language spa
dc.relation.none.fl_str_mv https://revistasinvestigacion.unmsm.edu.pe/index.php/veterinaria/article/view/13361/12270
dc.rights.none.fl_str_mv https://creativecommons.org/licenses/by-nc-sa/4.0
info:eu-repo/semantics/openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by-nc-sa/4.0
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Universidad Nacional Mayor de San Marcos, Facultad de Medicina Veterinaria
publisher.none.fl_str_mv Universidad Nacional Mayor de San Marcos, Facultad de Medicina Veterinaria
dc.source.none.fl_str_mv Revista de Investigaciones Veterinarias del Perú; Vol. 28 Núm. 3 (2017); 713-722
Revista de Investigaciones Veterinarias del Perú; Vol. 28 No. 3 (2017); 713-722
1682-3419
1609-9117
reponame:Revistas - Universidad Nacional Mayor de San Marcos
instname:Universidad Nacional Mayor de San Marcos
instacron:UNMSM
instname_str Universidad Nacional Mayor de San Marcos
instacron_str UNMSM
institution UNMSM
reponame_str Revistas - Universidad Nacional Mayor de San Marcos
collection Revistas - Universidad Nacional Mayor de San Marcos
repository.name.fl_str_mv
repository.mail.fl_str_mv
_version_ 1795238228439072768
score 13.888049
Evaluation of a Multiplex-PCR method for rapid detection of Salmonella Typhimurium and Enteritidis in naturally infected guinea pigs (Cavia porcellus)
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