Determination of the optimum concentration of three cryoprotectants for the criopreservation of alpaca epididymal spermatozoa
Descripción del Articulo
The aim of this study was to determine the optimal concentration of glycerol, ethylene glycol and dimethyl sulfoxide (DMSO) in the cryopreservation of epididymal spermatozoa of alpaca. The effect of different concentrations of glycerol, ethylene glycol and DMSO: T1 (0 M), T2 (0.25 M), T3 (0.5 M), T4...
| Autores: | , , , , |
|---|---|
| Formato: | artículo |
| Fecha de Publicación: | 2017 |
| Institución: | Universidad Nacional Mayor de San Marcos |
| Repositorio: | Revistas - Universidad Nacional Mayor de San Marcos |
| Lenguaje: | español |
| OAI Identifier: | oai:ojs.csi.unmsm:article/13367 |
| Enlace del recurso: | https://revistasinvestigacion.unmsm.edu.pe/index.php/veterinaria/article/view/13367 |
| Nivel de acceso: | acceso abierto |
| Materia: | spermatozoa epididymis alpaca cryopreservation espermatozoide epidídimo criopreservación |
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Revistas - Universidad Nacional Mayor de San Marcos |
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| dc.title.none.fl_str_mv |
Determination of the optimum concentration of three cryoprotectants for the criopreservation of alpaca epididymal spermatozoa Determinación de la Concentración Óptima de Tres Crioprotectores para la Criopreservación de Espermatozoides Epididimarios de Alpaca |
| title |
Determination of the optimum concentration of three cryoprotectants for the criopreservation of alpaca epididymal spermatozoa |
| spellingShingle |
Determination of the optimum concentration of three cryoprotectants for the criopreservation of alpaca epididymal spermatozoa Choez A., Katherine spermatozoa epididymis alpaca cryopreservation espermatozoide epidídimo alpaca criopreservación |
| title_short |
Determination of the optimum concentration of three cryoprotectants for the criopreservation of alpaca epididymal spermatozoa |
| title_full |
Determination of the optimum concentration of three cryoprotectants for the criopreservation of alpaca epididymal spermatozoa |
| title_fullStr |
Determination of the optimum concentration of three cryoprotectants for the criopreservation of alpaca epididymal spermatozoa |
| title_full_unstemmed |
Determination of the optimum concentration of three cryoprotectants for the criopreservation of alpaca epididymal spermatozoa |
| title_sort |
Determination of the optimum concentration of three cryoprotectants for the criopreservation of alpaca epididymal spermatozoa |
| dc.creator.none.fl_str_mv |
Choez A., Katherine Ruiz G., Luis Sandoval M., Rocío Evangelista V., Shirley Santiani A., Alexei |
| author |
Choez A., Katherine |
| author_facet |
Choez A., Katherine Ruiz G., Luis Sandoval M., Rocío Evangelista V., Shirley Santiani A., Alexei |
| author_role |
author |
| author2 |
Ruiz G., Luis Sandoval M., Rocío Evangelista V., Shirley Santiani A., Alexei |
| author2_role |
author author author author |
| dc.subject.none.fl_str_mv |
spermatozoa epididymis alpaca cryopreservation espermatozoide epidídimo alpaca criopreservación |
| topic |
spermatozoa epididymis alpaca cryopreservation espermatozoide epidídimo alpaca criopreservación |
| description |
The aim of this study was to determine the optimal concentration of glycerol, ethylene glycol and dimethyl sulfoxide (DMSO) in the cryopreservation of epididymal spermatozoa of alpaca. The effect of different concentrations of glycerol, ethylene glycol and DMSO: T1 (0 M), T2 (0.25 M), T3 (0.5 M), T4 (0.75 M), T5 (1 M), T6 (1.25 M), T7 (1.5 M) and T8 (1.75 M) was evaluated. A polynomial regression model was used to determine the best concentration of each cryoprotectant, resulting 1.16, 1.02 and 0.9 M the optimal concentrations for glycerol, ethylene glycol and DMSO respectively. In Experiment 2, the effect of the optimal concentrations between glycerol, ethylene glycol and DMSO on motility, plasma membrane integrity, and acrosomal viability and integrity in alpaca epididymal spermatozoa were analyzed. The results showed a mean of 35.8, 51.8 and 38.3% for DMSO and 30.8, 45.0 and 33.8% for glycerol for motility, plasma membrane integrity and acrosome viability and integrity, respectively, and without significant differences between these cryoprotectants. However, the results were significantly better (p<0.05) than for ethylene glycol (1.02 M). It is concluded that the concentrations of 0.9 M DMSO and 1.16 M glycerol better preserved the functional characteristics of alpaca epididymal spermatozoa as compared to ethylene glycol (1.02 M). |
| publishDate |
2017 |
| dc.date.none.fl_str_mv |
2017-10-11 |
| dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
| format |
article |
| status_str |
publishedVersion |
| dc.identifier.none.fl_str_mv |
https://revistasinvestigacion.unmsm.edu.pe/index.php/veterinaria/article/view/13367 10.15381/rivep.v28i3.13367 |
| url |
https://revistasinvestigacion.unmsm.edu.pe/index.php/veterinaria/article/view/13367 |
| identifier_str_mv |
10.15381/rivep.v28i3.13367 |
| dc.language.none.fl_str_mv |
spa |
| language |
spa |
| dc.relation.none.fl_str_mv |
https://revistasinvestigacion.unmsm.edu.pe/index.php/veterinaria/article/view/13367/12257 |
| dc.rights.none.fl_str_mv |
https://creativecommons.org/licenses/by-nc-sa/4.0 info:eu-repo/semantics/openAccess |
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https://creativecommons.org/licenses/by-nc-sa/4.0 |
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openAccess |
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application/pdf |
| dc.publisher.none.fl_str_mv |
Universidad Nacional Mayor de San Marcos, Facultad de Medicina Veterinaria |
| publisher.none.fl_str_mv |
Universidad Nacional Mayor de San Marcos, Facultad de Medicina Veterinaria |
| dc.source.none.fl_str_mv |
Revista de Investigaciones Veterinarias del Perú; Vol. 28 Núm. 3 (2017); 619-628 Revista de Investigaciones Veterinarias del Perú; Vol. 28 No. 3 (2017); 619-628 1682-3419 1609-9117 reponame:Revistas - Universidad Nacional Mayor de San Marcos instname:Universidad Nacional Mayor de San Marcos instacron:UNMSM |
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Universidad Nacional Mayor de San Marcos |
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UNMSM |
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UNMSM |
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Revistas - Universidad Nacional Mayor de San Marcos |
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Revistas - Universidad Nacional Mayor de San Marcos |
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1795238228456898560 |
| spelling |
Determination of the optimum concentration of three cryoprotectants for the criopreservation of alpaca epididymal spermatozoaDeterminación de la Concentración Óptima de Tres Crioprotectores para la Criopreservación de Espermatozoides Epididimarios de AlpacaChoez A., KatherineRuiz G., LuisSandoval M., RocíoEvangelista V., ShirleySantiani A., AlexeispermatozoaepididymisalpacacryopreservationespermatozoideepidídimoalpacacriopreservaciónThe aim of this study was to determine the optimal concentration of glycerol, ethylene glycol and dimethyl sulfoxide (DMSO) in the cryopreservation of epididymal spermatozoa of alpaca. The effect of different concentrations of glycerol, ethylene glycol and DMSO: T1 (0 M), T2 (0.25 M), T3 (0.5 M), T4 (0.75 M), T5 (1 M), T6 (1.25 M), T7 (1.5 M) and T8 (1.75 M) was evaluated. A polynomial regression model was used to determine the best concentration of each cryoprotectant, resulting 1.16, 1.02 and 0.9 M the optimal concentrations for glycerol, ethylene glycol and DMSO respectively. In Experiment 2, the effect of the optimal concentrations between glycerol, ethylene glycol and DMSO on motility, plasma membrane integrity, and acrosomal viability and integrity in alpaca epididymal spermatozoa were analyzed. The results showed a mean of 35.8, 51.8 and 38.3% for DMSO and 30.8, 45.0 and 33.8% for glycerol for motility, plasma membrane integrity and acrosome viability and integrity, respectively, and without significant differences between these cryoprotectants. However, the results were significantly better (p<0.05) than for ethylene glycol (1.02 M). It is concluded that the concentrations of 0.9 M DMSO and 1.16 M glycerol better preserved the functional characteristics of alpaca epididymal spermatozoa as compared to ethylene glycol (1.02 M).El objetivo del presente estudio fue determinar la concentración óptima de glicerol, etilenglicol y dimetil sulfóxido (DMSO) en la criopreservación de espermatozoides epididimarios de alpaca. En el experimento 1 se evaluó el efecto de ocho concentraciones de glicerol, etilenglicol y DMSO: T1 (0 M), T2 (0.25 M), T3 (0.5 M), T4 (0.75 M), T5 (1 M), T6 (1.25 M), T7 (1.5 M) y T8 (1.75 M). Se utilizó un modelo de regresión polinomial quíntuple para determinar la mejor concentración de cada crioprotector, resultando concentraciones óptimas para glicerol, etilenglicol y DMSO de 1.16, 1.02 y 0.9 M, respectivamente. En el Experimento 2, se comparó el efecto de las concentraciones óptimas entre glicerol, etilenglicol y DMSO sobre la motilidad, integridad de membrana plasmática y viabilidad e integridad acrosomal en los espermatozoides epididimarios de alpaca. Los resultados mostraron promedios de 35.8, 51.8 y 38.3% para DMSO (0.9 M) y de de 30.8, 45.0 y 33.8% para glicerol para las variables motilidad, integridad de membrana plasmática y viabilidad e integridad acrosomal, respectivamente, y sin diferencias significativas entre estos crioprotectores. Sin embargo, fueron significativamente mejores (p<0.05) que para etilenglicol (1.02 M). Se concluye que las concentraciones de 0.9 M de DMSO y 1.16 M de glicerol conservaron mejor las características funcionales de los espermatozoides epididimarios de alpaca en comparación con etilenglicol (1.02 M).Universidad Nacional Mayor de San Marcos, Facultad de Medicina Veterinaria2017-10-11info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://revistasinvestigacion.unmsm.edu.pe/index.php/veterinaria/article/view/1336710.15381/rivep.v28i3.13367Revista de Investigaciones Veterinarias del Perú; Vol. 28 Núm. 3 (2017); 619-628Revista de Investigaciones Veterinarias del Perú; Vol. 28 No. 3 (2017); 619-6281682-34191609-9117reponame:Revistas - Universidad Nacional Mayor de San Marcosinstname:Universidad Nacional Mayor de San Marcosinstacron:UNMSMspahttps://revistasinvestigacion.unmsm.edu.pe/index.php/veterinaria/article/view/13367/12257Derechos de autor 2017 Katherine Choez A., Luis Ruiz G., Rocío Sandoval M., Shirley Evangelista V., Alexei Santiani A.https://creativecommons.org/licenses/by-nc-sa/4.0info:eu-repo/semantics/openAccessoai:ojs.csi.unmsm:article/133672017-12-19T16:57:15Z |
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13.958958 |
Nota importante:
La información contenida en este registro es de entera responsabilidad de la institución que gestiona el repositorio institucional donde esta contenido este documento o set de datos. El CONCYTEC no se hace responsable por los contenidos (publicaciones y/o datos) accesibles a través del Repositorio Nacional Digital de Ciencia, Tecnología e Innovación de Acceso Abierto (ALICIA).
La información contenida en este registro es de entera responsabilidad de la institución que gestiona el repositorio institucional donde esta contenido este documento o set de datos. El CONCYTEC no se hace responsable por los contenidos (publicaciones y/o datos) accesibles a través del Repositorio Nacional Digital de Ciencia, Tecnología e Innovación de Acceso Abierto (ALICIA).
