A co-culture system with cumulus oophurus cells improves the quality of bovine embryos produced in vitro
Descripción del Articulo
The aim of this study was to determine the influence of a co-culture system with cumulus oophorus cells on the quality of bovine embryos produced in vitro, based on their viability. Ovaries from crossbred bovine slaughtered at the Chachapoyas Municipal Benefit Centre, Amazonas, Peru, were used. Oocy...
| Autores: | , , , |
|---|---|
| Formato: | artículo |
| Fecha de Publicación: | 2020 |
| Institución: | Universidad Nacional Mayor de San Marcos |
| Repositorio: | Revistas - Universidad Nacional Mayor de San Marcos |
| Lenguaje: | español |
| OAI Identifier: | oai:ojs.csi.unmsm:article/18181 |
| Enlace del recurso: | https://revistasinvestigacion.unmsm.edu.pe/index.php/veterinaria/article/view/18181 |
| Nivel de acceso: | acceso abierto |
| Materia: | cumulus oophurus in vitro fertilization in vitro culture embryonic viability enzymatic activity fecundación in vitro cultivo in vitro viabilidad embrionaria actividad enzimática |
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Revistas - Universidad Nacional Mayor de San Marcos |
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A co-culture system with cumulus oophurus cells improves the quality of bovine embryos produced in vitro Un sistema de co-cultivo con células del cumulus oophurus mejora la calidad de embriones bovinos producidos in vitro |
| title |
A co-culture system with cumulus oophurus cells improves the quality of bovine embryos produced in vitro |
| spellingShingle |
A co-culture system with cumulus oophurus cells improves the quality of bovine embryos produced in vitro Guevara-Chacón, Susana cumulus oophurus in vitro fertilization in vitro culture embryonic viability enzymatic activity cumulus oophurus fecundación in vitro cultivo in vitro viabilidad embrionaria actividad enzimática |
| title_short |
A co-culture system with cumulus oophurus cells improves the quality of bovine embryos produced in vitro |
| title_full |
A co-culture system with cumulus oophurus cells improves the quality of bovine embryos produced in vitro |
| title_fullStr |
A co-culture system with cumulus oophurus cells improves the quality of bovine embryos produced in vitro |
| title_full_unstemmed |
A co-culture system with cumulus oophurus cells improves the quality of bovine embryos produced in vitro |
| title_sort |
A co-culture system with cumulus oophurus cells improves the quality of bovine embryos produced in vitro |
| dc.creator.none.fl_str_mv |
Guevara-Chacón, Susana Quispe-Ccasa, Hurley Abel Saucedo-Uriarte, José Américo Cayo-Colca, Ilse Silvia |
| author |
Guevara-Chacón, Susana |
| author_facet |
Guevara-Chacón, Susana Quispe-Ccasa, Hurley Abel Saucedo-Uriarte, José Américo Cayo-Colca, Ilse Silvia |
| author_role |
author |
| author2 |
Quispe-Ccasa, Hurley Abel Saucedo-Uriarte, José Américo Cayo-Colca, Ilse Silvia |
| author2_role |
author author author |
| dc.subject.none.fl_str_mv |
cumulus oophurus in vitro fertilization in vitro culture embryonic viability enzymatic activity cumulus oophurus fecundación in vitro cultivo in vitro viabilidad embrionaria actividad enzimática |
| topic |
cumulus oophurus in vitro fertilization in vitro culture embryonic viability enzymatic activity cumulus oophurus fecundación in vitro cultivo in vitro viabilidad embrionaria actividad enzimática |
| description |
The aim of this study was to determine the influence of a co-culture system with cumulus oophorus cells on the quality of bovine embryos produced in vitro, based on their viability. Ovaries from crossbred bovine slaughtered at the Chachapoyas Municipal Benefit Centre, Amazonas, Peru, were used. Oocytes aspirated from 2-6 mm follicles were matured in TCM-199 medium for 24 hours, in a humidified atmosphere with 6% CO2 and 38.5 °C. The maturation state was determined by the nuclear stage through aceto-orcein staining. The mature oocytes were fertilized in vitro with frozen semen from Angus bulls for 18 hours. The presumed zygotes were randomly distributed for development in one group in medium with co-culture and another in medium without co-culture (control). The embryonic quality was determined based on the enzymatic activity and DNA damage of the embryos on the seventh day of culture, using the fluorochromes fluorescein diacetate (FDA) and propidium iodide (PI), respectively. No significant association (p>0.05) was found in the number of embryos produced in vitro, but there was a significant difference in embryonic viability in favour of the system with co-culture (p<0.05). The use of co-cultures with cumulus oophurus cells improves viability and, therefore, embryonic quality, without influencing the rate of embryo production in vitro. |
| publishDate |
2020 |
| dc.date.none.fl_str_mv |
2020-08-10 |
| dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
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article |
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publishedVersion |
| dc.identifier.none.fl_str_mv |
https://revistasinvestigacion.unmsm.edu.pe/index.php/veterinaria/article/view/18181 10.15381/rivep.v31i3.18181 |
| url |
https://revistasinvestigacion.unmsm.edu.pe/index.php/veterinaria/article/view/18181 |
| identifier_str_mv |
10.15381/rivep.v31i3.18181 |
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spa |
| language |
spa |
| dc.relation.none.fl_str_mv |
https://revistasinvestigacion.unmsm.edu.pe/index.php/veterinaria/article/view/18181/15490 |
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https://creativecommons.org/licenses/by-nc-sa/4.0 info:eu-repo/semantics/openAccess |
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https://creativecommons.org/licenses/by-nc-sa/4.0 |
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openAccess |
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application/pdf |
| dc.publisher.none.fl_str_mv |
Universidad Nacional Mayor de San Marcos, Facultad de Medicina Veterinaria |
| publisher.none.fl_str_mv |
Universidad Nacional Mayor de San Marcos, Facultad de Medicina Veterinaria |
| dc.source.none.fl_str_mv |
Revista de Investigaciones Veterinarias del Perú; Vol. 31 Núm. 3 (2020); e18181 Revista de Investigaciones Veterinarias del Perú; Vol. 31 No. 3 (2020); e18181 1682-3419 1609-9117 reponame:Revistas - Universidad Nacional Mayor de San Marcos instname:Universidad Nacional Mayor de San Marcos instacron:UNMSM |
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Universidad Nacional Mayor de San Marcos |
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UNMSM |
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UNMSM |
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Revistas - Universidad Nacional Mayor de San Marcos |
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Revistas - Universidad Nacional Mayor de San Marcos |
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1795238234078314496 |
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A co-culture system with cumulus oophurus cells improves the quality of bovine embryos produced in vitroUn sistema de co-cultivo con células del cumulus oophurus mejora la calidad de embriones bovinos producidos in vitroGuevara-Chacón, SusanaQuispe-Ccasa, Hurley AbelSaucedo-Uriarte, José AméricoCayo-Colca, Ilse Silviacumulus oophurusin vitro fertilizationin vitro cultureembryonic viabilityenzymatic activitycumulus oophurusfecundación in vitrocultivo in vitroviabilidad embrionariaactividad enzimáticaThe aim of this study was to determine the influence of a co-culture system with cumulus oophorus cells on the quality of bovine embryos produced in vitro, based on their viability. Ovaries from crossbred bovine slaughtered at the Chachapoyas Municipal Benefit Centre, Amazonas, Peru, were used. Oocytes aspirated from 2-6 mm follicles were matured in TCM-199 medium for 24 hours, in a humidified atmosphere with 6% CO2 and 38.5 °C. The maturation state was determined by the nuclear stage through aceto-orcein staining. The mature oocytes were fertilized in vitro with frozen semen from Angus bulls for 18 hours. The presumed zygotes were randomly distributed for development in one group in medium with co-culture and another in medium without co-culture (control). The embryonic quality was determined based on the enzymatic activity and DNA damage of the embryos on the seventh day of culture, using the fluorochromes fluorescein diacetate (FDA) and propidium iodide (PI), respectively. No significant association (p>0.05) was found in the number of embryos produced in vitro, but there was a significant difference in embryonic viability in favour of the system with co-culture (p<0.05). The use of co-cultures with cumulus oophurus cells improves viability and, therefore, embryonic quality, without influencing the rate of embryo production in vitro.El estudio tuvo como objetivo determinar la influencia de un sistema de co-cultivo con células del cumulus oophorus sobre la calidad de embriones bovinos producidos in vitro en función a su viabilidad. Se utilizaron ovarios de bovinos hembra cruzadas, sacrificadas en el Centro de Beneficio Municipal de Chachapoyas, Amazonas, Perú. Los ovocitos aspirados de folículos de 2-6 mm fueron madurados en medio TCM-199 por 24 horas, en una atmósfera humidificada con 6% de CO2 y a 38.5 °C. El estado de maduración fue determinado mediante el estadio nuclear a través de tinción aceto-orceína. Los ovocitos maduros se fertilizaron in vitro con semen congelado de toros Angus durante 18 horas. Los presuntos cigotos fueron distribuidos aleatoriamente para su desarrollo en un grupo en medio con co-cultivo y otro en medio sin co-cultivo (control). Se determinó la calidad embrionaria en función a la actividad enzimática y daño al ADN de los embriones al séptimo día del cultivo, mediante los fluorocromos diacetato de fluoresceína (FDA) y yoduro de propidio (PI), respectivamente. No se encontró asociación significativa (p>0.05) en la cantidad de embriones producidos in vitro, pero hubo diferencia significativa en viabilidad embrionaria a favor del sistema con co-cultivo (p<0.05). El uso de co-cultivos con células de cumulus oophurus mejora la viabilidad y, por lo tanto, la calidad embrionaria, sin influir en la tasa de producción de embriones in vitro.Universidad Nacional Mayor de San Marcos, Facultad de Medicina Veterinaria2020-08-10info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://revistasinvestigacion.unmsm.edu.pe/index.php/veterinaria/article/view/1818110.15381/rivep.v31i3.18181Revista de Investigaciones Veterinarias del Perú; Vol. 31 Núm. 3 (2020); e18181Revista de Investigaciones Veterinarias del Perú; Vol. 31 No. 3 (2020); e181811682-34191609-9117reponame:Revistas - Universidad Nacional Mayor de San Marcosinstname:Universidad Nacional Mayor de San Marcosinstacron:UNMSMspahttps://revistasinvestigacion.unmsm.edu.pe/index.php/veterinaria/article/view/18181/15490Derechos de autor 2020 Susana Guevara-Chacón, Hurley Abel Quispe-Ccasa, José Américo Saucedo-Uriarte, Ilse Silvia Cayo-Colcahttps://creativecommons.org/licenses/by-nc-sa/4.0info:eu-repo/semantics/openAccessoai:ojs.csi.unmsm:article/181812020-08-11T16:20:53Z |
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13.945474 |
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La información contenida en este registro es de entera responsabilidad de la institución que gestiona el repositorio institucional donde esta contenido este documento o set de datos. El CONCYTEC no se hace responsable por los contenidos (publicaciones y/o datos) accesibles a través del Repositorio Nacional Digital de Ciencia, Tecnología e Innovación de Acceso Abierto (ALICIA).
La información contenida en este registro es de entera responsabilidad de la institución que gestiona el repositorio institucional donde esta contenido este documento o set de datos. El CONCYTEC no se hace responsable por los contenidos (publicaciones y/o datos) accesibles a través del Repositorio Nacional Digital de Ciencia, Tecnología e Innovación de Acceso Abierto (ALICIA).
