Morphological and molecular identification of ticks collected from dogs (Canis lupus familiaris) with ehrlichiosis in Chiclayo, Peru
Descripción del Articulo
The present study aimed to identify morphologically and molecularly the ticks of dogs with ehrlichiosis in the Chiclayo district (Lambayeque, Peru). A total of 297 ticks from 74 dogs were collected and morphologically identified as Rhipicephalus sanguineus s.l. Nine specimens were used for molecular...
| Autores: | , , , , , , , |
|---|---|
| Formato: | artículo |
| Fecha de Publicación: | 2020 |
| Institución: | Universidad Nacional Mayor de San Marcos |
| Repositorio: | Revistas - Universidad Nacional Mayor de San Marcos |
| Lenguaje: | español |
| OAI Identifier: | oai:ojs.csi.unmsm:article/17820 |
| Enlace del recurso: | https://revistasinvestigacion.unmsm.edu.pe/index.php/veterinaria/article/view/17820 |
| Nivel de acceso: | acceso abierto |
| Materia: | ticks Rhipicephalus sanguineus s.l 16S rDNA gene Lineage of the North Ehrlichia canis garrapata gen 16S rADN Linaje del Norte |
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Morphological and molecular identification of ticks collected from dogs (Canis lupus familiaris) with ehrlichiosis in Chiclayo, PeruIdentificación morfológica y molecular de garrapatas colectadas de perros (Canis lupus familiaris) con ehrlichiosis en Chiclayo, PerúCervantes S., MiguelMasgo C., DeysiRamírez V., LeylaÁlvarez M., GreciaLi E., OlgaVásquez-Ydrogo., AlvaroGomez-Puerta, Luis A.Hoyos S., LuisticksRhipicephalus sanguineus s.l16S rDNA geneLineage of the NorthEhrlichia canisgarrapataRhipicephalus sanguineus s.lgen 16S rADNLinaje del NorteEhrlichia canisThe present study aimed to identify morphologically and molecularly the ticks of dogs with ehrlichiosis in the Chiclayo district (Lambayeque, Peru). A total of 297 ticks from 74 dogs were collected and morphologically identified as Rhipicephalus sanguineus s.l. Nine specimens were used for molecular identification using the 16S rDNA ribosomal gene. The sequences obtained were aligned with sequences of R. sanguineus s.l. from Brazil and Colombia (Northern Lineage), Argentina, Chile and Uruguay (Southern Lineage), Spain, Italy and South Africa, showing a genetic divergence of 0-0.3% and 6.1-6.8% with the Northern Lineage and Southern Lineage, respectively. The phylogenetic analysis grouped the sequences of Peru with those of the Northern Lineage and South Africa, segregating them from the sequences of the Southern Lineage and Europe. The results show that R. sanguineus s.l. from Chiclayo, Peru, belongs to the Northern Lineage. El presente estudio tuvo como objetivo identificar morfológica y molecularmente las garrapatas de perros con ehrlichiosis en el distrito de Chiclayo (Lambayeque, Perú). Se colectaron 297 garrapatas de 74 perros y fueron identificadas morfológicamente como Rhipicephalus sanguineus s.l. Nueve especímenes fueron utilizados para la identificación molecular usando el gen ribosomal 16S rADN. Las secuencias obtenidas fueron alineadas con secuencias de R. sanguineus s.l de Brasil y Colombia (Linaje del Norte), Argentina, Chile y Uruguay (Linaje del Sur), España, Italia y Sudáfrica, mostrando una divergencia genética de 0-0.3% y 6.1-6.8% con el Linaje del Norte y Linaje del Sur, respectivamente. El análisis filogenético agrupó las secuencias del Perú con las del Linaje del Norte y Sudáfrica, segregándolas de las secuencias del Linaje del Sur y Europa. Los resultados demuestran que R. sanguineus s.l de Chiclayo, Perú, pertenece al Linaje del Norte. Universidad Nacional Mayor de San Marcos, Facultad de Medicina Veterinaria2020-06-20info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://revistasinvestigacion.unmsm.edu.pe/index.php/veterinaria/article/view/1782010.15381/rivep.v31i2.17820Revista de Investigaciones Veterinarias del Perú; Vol. 31 Núm. 2 (2020); e17820Revista de Investigaciones Veterinarias del Perú; Vol. 31 No. 2 (2020); e178201682-34191609-9117reponame:Revistas - Universidad Nacional Mayor de San Marcosinstname:Universidad Nacional Mayor de San Marcosinstacron:UNMSMspahttps://revistasinvestigacion.unmsm.edu.pe/index.php/veterinaria/article/view/17820/15101Derechos de autor 2020 Miguel Cervantes S., Deysi Masgo C., Leyla Ramírez V., Grecia Álvarez M., Olga Li E., Alvaro Vásquez-Ydrogo., Luis A. Gomez-Puerta, Luis Hoyos S.https://creativecommons.org/licenses/by-nc-sa/4.0info:eu-repo/semantics/openAccessoai:ojs.csi.unmsm:article/178202020-06-23T14:46:03Z |
| dc.title.none.fl_str_mv |
Morphological and molecular identification of ticks collected from dogs (Canis lupus familiaris) with ehrlichiosis in Chiclayo, Peru Identificación morfológica y molecular de garrapatas colectadas de perros (Canis lupus familiaris) con ehrlichiosis en Chiclayo, Perú |
| title |
Morphological and molecular identification of ticks collected from dogs (Canis lupus familiaris) with ehrlichiosis in Chiclayo, Peru |
| spellingShingle |
Morphological and molecular identification of ticks collected from dogs (Canis lupus familiaris) with ehrlichiosis in Chiclayo, Peru Cervantes S., Miguel ticks Rhipicephalus sanguineus s.l 16S rDNA gene Lineage of the North Ehrlichia canis garrapata Rhipicephalus sanguineus s.l gen 16S rADN Linaje del Norte Ehrlichia canis |
| title_short |
Morphological and molecular identification of ticks collected from dogs (Canis lupus familiaris) with ehrlichiosis in Chiclayo, Peru |
| title_full |
Morphological and molecular identification of ticks collected from dogs (Canis lupus familiaris) with ehrlichiosis in Chiclayo, Peru |
| title_fullStr |
Morphological and molecular identification of ticks collected from dogs (Canis lupus familiaris) with ehrlichiosis in Chiclayo, Peru |
| title_full_unstemmed |
Morphological and molecular identification of ticks collected from dogs (Canis lupus familiaris) with ehrlichiosis in Chiclayo, Peru |
| title_sort |
Morphological and molecular identification of ticks collected from dogs (Canis lupus familiaris) with ehrlichiosis in Chiclayo, Peru |
| dc.creator.none.fl_str_mv |
Cervantes S., Miguel Masgo C., Deysi Ramírez V., Leyla Álvarez M., Grecia Li E., Olga Vásquez-Ydrogo., Alvaro Gomez-Puerta, Luis A. Hoyos S., Luis |
| author |
Cervantes S., Miguel |
| author_facet |
Cervantes S., Miguel Masgo C., Deysi Ramírez V., Leyla Álvarez M., Grecia Li E., Olga Vásquez-Ydrogo., Alvaro Gomez-Puerta, Luis A. Hoyos S., Luis |
| author_role |
author |
| author2 |
Masgo C., Deysi Ramírez V., Leyla Álvarez M., Grecia Li E., Olga Vásquez-Ydrogo., Alvaro Gomez-Puerta, Luis A. Hoyos S., Luis |
| author2_role |
author author author author author author author |
| dc.subject.none.fl_str_mv |
ticks Rhipicephalus sanguineus s.l 16S rDNA gene Lineage of the North Ehrlichia canis garrapata Rhipicephalus sanguineus s.l gen 16S rADN Linaje del Norte Ehrlichia canis |
| topic |
ticks Rhipicephalus sanguineus s.l 16S rDNA gene Lineage of the North Ehrlichia canis garrapata Rhipicephalus sanguineus s.l gen 16S rADN Linaje del Norte Ehrlichia canis |
| description |
The present study aimed to identify morphologically and molecularly the ticks of dogs with ehrlichiosis in the Chiclayo district (Lambayeque, Peru). A total of 297 ticks from 74 dogs were collected and morphologically identified as Rhipicephalus sanguineus s.l. Nine specimens were used for molecular identification using the 16S rDNA ribosomal gene. The sequences obtained were aligned with sequences of R. sanguineus s.l. from Brazil and Colombia (Northern Lineage), Argentina, Chile and Uruguay (Southern Lineage), Spain, Italy and South Africa, showing a genetic divergence of 0-0.3% and 6.1-6.8% with the Northern Lineage and Southern Lineage, respectively. The phylogenetic analysis grouped the sequences of Peru with those of the Northern Lineage and South Africa, segregating them from the sequences of the Southern Lineage and Europe. The results show that R. sanguineus s.l. from Chiclayo, Peru, belongs to the Northern Lineage. |
| publishDate |
2020 |
| dc.date.none.fl_str_mv |
2020-06-20 |
| dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
| format |
article |
| status_str |
publishedVersion |
| dc.identifier.none.fl_str_mv |
https://revistasinvestigacion.unmsm.edu.pe/index.php/veterinaria/article/view/17820 10.15381/rivep.v31i2.17820 |
| url |
https://revistasinvestigacion.unmsm.edu.pe/index.php/veterinaria/article/view/17820 |
| identifier_str_mv |
10.15381/rivep.v31i2.17820 |
| dc.language.none.fl_str_mv |
spa |
| language |
spa |
| dc.relation.none.fl_str_mv |
https://revistasinvestigacion.unmsm.edu.pe/index.php/veterinaria/article/view/17820/15101 |
| dc.rights.none.fl_str_mv |
https://creativecommons.org/licenses/by-nc-sa/4.0 info:eu-repo/semantics/openAccess |
| rights_invalid_str_mv |
https://creativecommons.org/licenses/by-nc-sa/4.0 |
| eu_rights_str_mv |
openAccess |
| dc.format.none.fl_str_mv |
application/pdf |
| dc.publisher.none.fl_str_mv |
Universidad Nacional Mayor de San Marcos, Facultad de Medicina Veterinaria |
| publisher.none.fl_str_mv |
Universidad Nacional Mayor de San Marcos, Facultad de Medicina Veterinaria |
| dc.source.none.fl_str_mv |
Revista de Investigaciones Veterinarias del Perú; Vol. 31 Núm. 2 (2020); e17820 Revista de Investigaciones Veterinarias del Perú; Vol. 31 No. 2 (2020); e17820 1682-3419 1609-9117 reponame:Revistas - Universidad Nacional Mayor de San Marcos instname:Universidad Nacional Mayor de San Marcos instacron:UNMSM |
| instname_str |
Universidad Nacional Mayor de San Marcos |
| instacron_str |
UNMSM |
| institution |
UNMSM |
| reponame_str |
Revistas - Universidad Nacional Mayor de San Marcos |
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Revistas - Universidad Nacional Mayor de San Marcos |
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| repository.mail.fl_str_mv |
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1795238233289785344 |
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13.903862 |
Nota importante:
La información contenida en este registro es de entera responsabilidad de la institución que gestiona el repositorio institucional donde esta contenido este documento o set de datos. El CONCYTEC no se hace responsable por los contenidos (publicaciones y/o datos) accesibles a través del Repositorio Nacional Digital de Ciencia, Tecnología e Innovación de Acceso Abierto (ALICIA).
La información contenida en este registro es de entera responsabilidad de la institución que gestiona el repositorio institucional donde esta contenido este documento o set de datos. El CONCYTEC no se hace responsable por los contenidos (publicaciones y/o datos) accesibles a través del Repositorio Nacional Digital de Ciencia, Tecnología e Innovación de Acceso Abierto (ALICIA).
