Transference of some microsatellite molecular markers from Fabaceae family to Andean Lupin (Lupinus mutabilis Sweet)
Descripción del Articulo
In order to analyze transferibility of 15 microsatellite primers from Fabaceae family to Lupinus mutabilis Sweet "andean lupine" chosen to present transferability between species and genera, by its high rate of polymorphic content (PIC) and high degree of observed and expected heterozygosi...
Autores: | , |
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Formato: | artículo |
Fecha de Publicación: | 2015 |
Institución: | Universidad Nacional de Trujillo |
Repositorio: | Revistas - Universidad Nacional de Trujillo |
Lenguaje: | español |
OAI Identifier: | oai:ojs.revistas.unitru.edu.pe:article/811 |
Enlace del recurso: | https://revistas.unitru.edu.pe/index.php/scientiaagrop/article/view/811 |
Nivel de acceso: | acceso abierto |
Materia: | Lupinus mutabilis familia Fabaceae transferencia marcadores moleculares microsatélites Fabaceae family transference microsatellite molecular markers |
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Transference of some microsatellite molecular markers from Fabaceae family to Andean Lupin (Lupinus mutabilis Sweet)Transferencia de algunos marcadores moleculares microsatélites de la familia Fabaceae en tarwi (Lupinus mutabilis Sweet)Chirinos-Arias, Michelle C.Jiménez, Jorge E.Lupinus mutabilisfamilia Fabaceae transferenciamarcadores moleculares microsatélitesLupinus mutabilisFabaceae familytransferencemicrosatellite molecular markersIn order to analyze transferibility of 15 microsatellite primers from Fabaceae family to Lupinus mutabilis Sweet "andean lupine" chosen to present transferability between species and genera, by its high rate of polymorphic content (PIC) and high degree of observed and expected heterozygosity. DNA was extracted of 300 andean lupines plants, PCR conditions were standardized by gradients of master mix components. Primers for screening were run on 3% agarose gel with some samples. Finally population was amplified and run on 6% polyacrylamide gel for its highest resolution. Only 6.67% of primers were amplified, but they were monomorphic, so they cannot be used for molecular characterization. We proposed eight microsatellite primers for andean lupin wich should be probed in laboratory conditions.Con el objetivo de analizar la transferencia de 15 iniciadores microsatélites de la familia Fabaceae en Lupinus mutabilis Sweet "tarwi", escogidos por presentar transferibilidad entre especies y géneros, por su alto índice de contenido polimórfico (PIC) y alto grado de heterocigosidad observada y esperada. Se extrajo el ADN de 300 plantas de tarwi, se estandarizaron las condiciones PCR haciendo gradientes de los diferentes componentes del master mix. Para el screening se corrieron los iniciadores en gel de agarosa 3% con algunas muestras. Finalmente se amplificó la población y se corrieron en gel de poliacrilamida 6% por su mayor resolución. Sólo el 6,67% de iniciadores amplificaron pero resultaron ser monomórficos, por lo que no pueden usarse en caracterización molecular. Se propone ocho iniciadores microsatélites para tarwi, los cuales deben ser probados en condiciones de laboratorio.Universidad Nacional de Trujillo2015-03-31info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://revistas.unitru.edu.pe/index.php/scientiaagrop/article/view/811Scientia Agropecuaria; Vol. 6 Núm. 1 (2015): Enero - Marzo; 51-58Scientia Agropecuaria; Vol. 6 No. 1 (2015): January - March; 51-582306-67412077-9917reponame:Revistas - Universidad Nacional de Trujilloinstname:Universidad Nacional de Trujilloinstacron:UNITRUspahttps://revistas.unitru.edu.pe/index.php/scientiaagrop/article/view/811/740https://revistas.unitru.edu.pe/index.php/scientiaagrop/article/view/811/1360Derechos de autor 2015 Scientia Agropecuariainfo:eu-repo/semantics/openAccessoai:ojs.revistas.unitru.edu.pe:article/8112017-03-07T15:56:34Z |
dc.title.none.fl_str_mv |
Transference of some microsatellite molecular markers from Fabaceae family to Andean Lupin (Lupinus mutabilis Sweet) Transferencia de algunos marcadores moleculares microsatélites de la familia Fabaceae en tarwi (Lupinus mutabilis Sweet) |
title |
Transference of some microsatellite molecular markers from Fabaceae family to Andean Lupin (Lupinus mutabilis Sweet) |
spellingShingle |
Transference of some microsatellite molecular markers from Fabaceae family to Andean Lupin (Lupinus mutabilis Sweet) Chirinos-Arias, Michelle C. Lupinus mutabilis familia Fabaceae transferencia marcadores moleculares microsatélites Lupinus mutabilis Fabaceae family transference microsatellite molecular markers |
title_short |
Transference of some microsatellite molecular markers from Fabaceae family to Andean Lupin (Lupinus mutabilis Sweet) |
title_full |
Transference of some microsatellite molecular markers from Fabaceae family to Andean Lupin (Lupinus mutabilis Sweet) |
title_fullStr |
Transference of some microsatellite molecular markers from Fabaceae family to Andean Lupin (Lupinus mutabilis Sweet) |
title_full_unstemmed |
Transference of some microsatellite molecular markers from Fabaceae family to Andean Lupin (Lupinus mutabilis Sweet) |
title_sort |
Transference of some microsatellite molecular markers from Fabaceae family to Andean Lupin (Lupinus mutabilis Sweet) |
dc.creator.none.fl_str_mv |
Chirinos-Arias, Michelle C. Jiménez, Jorge E. |
author |
Chirinos-Arias, Michelle C. |
author_facet |
Chirinos-Arias, Michelle C. Jiménez, Jorge E. |
author_role |
author |
author2 |
Jiménez, Jorge E. |
author2_role |
author |
dc.subject.none.fl_str_mv |
Lupinus mutabilis familia Fabaceae transferencia marcadores moleculares microsatélites Lupinus mutabilis Fabaceae family transference microsatellite molecular markers |
topic |
Lupinus mutabilis familia Fabaceae transferencia marcadores moleculares microsatélites Lupinus mutabilis Fabaceae family transference microsatellite molecular markers |
description |
In order to analyze transferibility of 15 microsatellite primers from Fabaceae family to Lupinus mutabilis Sweet "andean lupine" chosen to present transferability between species and genera, by its high rate of polymorphic content (PIC) and high degree of observed and expected heterozygosity. DNA was extracted of 300 andean lupines plants, PCR conditions were standardized by gradients of master mix components. Primers for screening were run on 3% agarose gel with some samples. Finally population was amplified and run on 6% polyacrylamide gel for its highest resolution. Only 6.67% of primers were amplified, but they were monomorphic, so they cannot be used for molecular characterization. We proposed eight microsatellite primers for andean lupin wich should be probed in laboratory conditions. |
publishDate |
2015 |
dc.date.none.fl_str_mv |
2015-03-31 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.none.fl_str_mv |
https://revistas.unitru.edu.pe/index.php/scientiaagrop/article/view/811 |
url |
https://revistas.unitru.edu.pe/index.php/scientiaagrop/article/view/811 |
dc.language.none.fl_str_mv |
spa |
language |
spa |
dc.relation.none.fl_str_mv |
https://revistas.unitru.edu.pe/index.php/scientiaagrop/article/view/811/740 https://revistas.unitru.edu.pe/index.php/scientiaagrop/article/view/811/1360 |
dc.rights.none.fl_str_mv |
Derechos de autor 2015 Scientia Agropecuaria info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
Derechos de autor 2015 Scientia Agropecuaria |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Universidad Nacional de Trujillo |
publisher.none.fl_str_mv |
Universidad Nacional de Trujillo |
dc.source.none.fl_str_mv |
Scientia Agropecuaria; Vol. 6 Núm. 1 (2015): Enero - Marzo; 51-58 Scientia Agropecuaria; Vol. 6 No. 1 (2015): January - March; 51-58 2306-6741 2077-9917 reponame:Revistas - Universidad Nacional de Trujillo instname:Universidad Nacional de Trujillo instacron:UNITRU |
instname_str |
Universidad Nacional de Trujillo |
instacron_str |
UNITRU |
institution |
UNITRU |
reponame_str |
Revistas - Universidad Nacional de Trujillo |
collection |
Revistas - Universidad Nacional de Trujillo |
repository.name.fl_str_mv |
|
repository.mail.fl_str_mv |
|
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1846521095337803776 |
score |
13.377221 |
Nota importante:
La información contenida en este registro es de entera responsabilidad de la institución que gestiona el repositorio institucional donde esta contenido este documento o set de datos. El CONCYTEC no se hace responsable por los contenidos (publicaciones y/o datos) accesibles a través del Repositorio Nacional Digital de Ciencia, Tecnología e Innovación de Acceso Abierto (ALICIA).
La información contenida en este registro es de entera responsabilidad de la institución que gestiona el repositorio institucional donde esta contenido este documento o set de datos. El CONCYTEC no se hace responsable por los contenidos (publicaciones y/o datos) accesibles a través del Repositorio Nacional Digital de Ciencia, Tecnología e Innovación de Acceso Abierto (ALICIA).