Determination of plant growth promoting potential of enterobacteria isolated from the rhizosphere of maize (Zea mays L.)

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The yellow maize is the third most important crop in Peru and part of the chain maize-poultry-pig, significant impact on the national economic and social activity, however, in 2011, only 40% of the corn offered corresponded to the domestic industry. Looking for alternatives to reduce the use of chem...

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Detalles Bibliográficos
Autor: León Mendoza, Luis H.
Formato: artículo
Fecha de Publicación:2014
Institución:Universidad Nacional de Trujillo
Repositorio:Revistas - Universidad Nacional de Trujillo
Lenguaje:español
OAI Identifier:oai:ojs.revistas.unitru.edu.pe:article/731
Enlace del recurso:https://revistas.unitru.edu.pe/index.php/scientiaagrop/article/view/731
Nivel de acceso:acceso abierto
Materia:Corn
enterobacteria
fertilizer
promoting
growth
Maíz
enterobacterias
fertilizante
promotora
crecimiento
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network_acronym_str REVUNITRU
network_name_str Revistas - Universidad Nacional de Trujillo
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dc.title.none.fl_str_mv Determination of plant growth promoting potential of enterobacteria isolated from the rhizosphere of maize (Zea mays L.)
Determinación del potencial promotor del crecimiento vegetal de las enterobacterias aisladas de la rizósfera del cultivo de maíz (Zea mays L.)
title Determination of plant growth promoting potential of enterobacteria isolated from the rhizosphere of maize (Zea mays L.)
spellingShingle Determination of plant growth promoting potential of enterobacteria isolated from the rhizosphere of maize (Zea mays L.)
León Mendoza, Luis H.
Corn
enterobacteria
fertilizer
promoting
growth
Maíz
enterobacterias
fertilizante
promotora
crecimiento
title_short Determination of plant growth promoting potential of enterobacteria isolated from the rhizosphere of maize (Zea mays L.)
title_full Determination of plant growth promoting potential of enterobacteria isolated from the rhizosphere of maize (Zea mays L.)
title_fullStr Determination of plant growth promoting potential of enterobacteria isolated from the rhizosphere of maize (Zea mays L.)
title_full_unstemmed Determination of plant growth promoting potential of enterobacteria isolated from the rhizosphere of maize (Zea mays L.)
title_sort Determination of plant growth promoting potential of enterobacteria isolated from the rhizosphere of maize (Zea mays L.)
dc.creator.none.fl_str_mv León Mendoza, Luis H.
author León Mendoza, Luis H.
author_facet León Mendoza, Luis H.
author_role author
dc.subject.none.fl_str_mv Corn
enterobacteria
fertilizer
promoting
growth
Maíz
enterobacterias
fertilizante
promotora
crecimiento
topic Corn
enterobacteria
fertilizer
promoting
growth
Maíz
enterobacterias
fertilizante
promotora
crecimiento
description The yellow maize is the third most important crop in Peru and part of the chain maize-poultry-pig, significant impact on the national economic and social activity, however, in 2011, only 40% of the corn offered corresponded to the domestic industry. Looking for alternatives to reduce the use of chemical fertilizers, have performed investigations with plant growth promoting rhizobacteria. Bacteria were isolated from the rhizosphere of maize from districts Monsefú and Reque, Lambayeque. Dilution was made into sterile saline 0.87% NaCl w/v and plated on MacConkey agar, incubating at 30°C for 48 hours. 269 pure cultures of bacteria were obtained, the biochemical reaction was investigated in agar Triple sugar iron agar Iron Lysine, agar Citrate Simons, peptone broth, red broth methyl Voges-Proskauer and nitrate broth, was identified 66% as Enterobacteriaceae of genera Pantoea (49%), Klebsiella (17%), Kluyvera (16%), Serratia (11%), Citrobacter (4%) and Hafnia (3%). The native enterobacteria were quantified to 31.67 ppm of fixed nitrogen as ammonia; 54.25 ppm indole acetic acid and 4,78 ppm solubilized phosphorus, activity proteolytic and chitinolytic and antagonistic activity of Fusarium verticillioides were also determined. 16% of native enterobacteria did not affect the emergence of hard yellow maize, 77% affected positively and 7% affected negatively. In turn, none bacteria affected survival. Was demonstrated the potential plant growth promoter of enterobacteria isolated from field crops in the region of Lambayeque.
publishDate 2014
dc.date.none.fl_str_mv 2014-12-20
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://revistas.unitru.edu.pe/index.php/scientiaagrop/article/view/731
url https://revistas.unitru.edu.pe/index.php/scientiaagrop/article/view/731
dc.language.none.fl_str_mv spa
language spa
dc.relation.none.fl_str_mv https://revistas.unitru.edu.pe/index.php/scientiaagrop/article/view/731/666
https://revistas.unitru.edu.pe/index.php/scientiaagrop/article/view/731/1350
dc.rights.none.fl_str_mv Derechos de autor 2014 Scientia Agropecuaria
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Derechos de autor 2014 Scientia Agropecuaria
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Universidad Nacional de Trujillo
publisher.none.fl_str_mv Universidad Nacional de Trujillo
dc.source.none.fl_str_mv Scientia Agropecuaria; Vol. 5 Núm. 4 (2014): Octubre - Diciembre; 177-185
Scientia Agropecuaria; Vol. 5 No. 4 (2014): October - December; 177-185
2306-6741
2077-9917
reponame:Revistas - Universidad Nacional de Trujillo
instname:Universidad Nacional de Trujillo
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instname_str Universidad Nacional de Trujillo
instacron_str UNITRU
institution UNITRU
reponame_str Revistas - Universidad Nacional de Trujillo
collection Revistas - Universidad Nacional de Trujillo
repository.name.fl_str_mv
repository.mail.fl_str_mv
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spelling Determination of plant growth promoting potential of enterobacteria isolated from the rhizosphere of maize (Zea mays L.)Determinación del potencial promotor del crecimiento vegetal de las enterobacterias aisladas de la rizósfera del cultivo de maíz (Zea mays L.)León Mendoza, Luis H.CornenterobacteriafertilizerpromotinggrowthMaízenterobacteriasfertilizantepromotoracrecimientoThe yellow maize is the third most important crop in Peru and part of the chain maize-poultry-pig, significant impact on the national economic and social activity, however, in 2011, only 40% of the corn offered corresponded to the domestic industry. Looking for alternatives to reduce the use of chemical fertilizers, have performed investigations with plant growth promoting rhizobacteria. Bacteria were isolated from the rhizosphere of maize from districts Monsefú and Reque, Lambayeque. Dilution was made into sterile saline 0.87% NaCl w/v and plated on MacConkey agar, incubating at 30°C for 48 hours. 269 pure cultures of bacteria were obtained, the biochemical reaction was investigated in agar Triple sugar iron agar Iron Lysine, agar Citrate Simons, peptone broth, red broth methyl Voges-Proskauer and nitrate broth, was identified 66% as Enterobacteriaceae of genera Pantoea (49%), Klebsiella (17%), Kluyvera (16%), Serratia (11%), Citrobacter (4%) and Hafnia (3%). The native enterobacteria were quantified to 31.67 ppm of fixed nitrogen as ammonia; 54.25 ppm indole acetic acid and 4,78 ppm solubilized phosphorus, activity proteolytic and chitinolytic and antagonistic activity of Fusarium verticillioides were also determined. 16% of native enterobacteria did not affect the emergence of hard yellow maize, 77% affected positively and 7% affected negatively. In turn, none bacteria affected survival. Was demonstrated the potential plant growth promoter of enterobacteria isolated from field crops in the region of Lambayeque.El maíz amarillo duro es el tercer cultivo en importancia en el Perú y forma parte de la cadena maíz-avicultura-porcicultura, de impacto significativo en la actividad económica y social nacional, sin embargo, en el 2011, solo el 40% del maíz ofertado correspondió a la producción nacional. En busca de alternativas para disminuir el uso de fertilizantes químicos se realizan investigaciones con denominadas rizobacterias promotoras del crecimiento vegetal. Las bacterias se aislaron de la rizósfera de maíz procedentes de campos agrícolas de los distritos de Monsefú y Reque, Región Lambayeque. Se realizó una dilución en solución salina esterilizada: 0,87% NaCl, p/v y se sembró en agar Mac Conkey, incubando a 30°C por 48 horas. Se obtuvieron 269 cultivos puros de bacterias, se investigó su reacción bioquímica en agar Hierro Triple Azúcar, agar Hierro Lisina, agar Citrato de Simons, caldo peptonado, caldo rojo de metilo Voges-Proskauer y caldo nitrato, identificándose 66% como enterobacterias de los géneros Pantoea (49%), Klebsiella (17%), Kluyvera (16%), Serratia (11%), Citrobacter (4%) y Hafnia (3%). Con las enterobacterias nativas se cuantificó hasta 31,67 ppm de nitrógeno fijado como amonio; 54,25 ppm de ácido indol acético y 4,78 ppm de fósforo solubilizado, así como también se determinó actividad proteolítica, quitinolítica y antagónica a Fusarium verticillioides. El 16% de enterobacterias nativas no afectó la emergencia de maíz amarillo duro hibrido simple AGRI- 144, el 77% la afectó positivamente y el 7% la afectó negativamente. A su vez, ninguna bacteria afectó la sobrevivencia. Se demostró el potencial promotor del crecimiento vegetal de las enterobacterias aisladas de campos de cultivos de la Región de Lambayeque.Universidad Nacional de Trujillo2014-12-20info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://revistas.unitru.edu.pe/index.php/scientiaagrop/article/view/731Scientia Agropecuaria; Vol. 5 Núm. 4 (2014): Octubre - Diciembre; 177-185Scientia Agropecuaria; Vol. 5 No. 4 (2014): October - December; 177-1852306-67412077-9917reponame:Revistas - Universidad Nacional de Trujilloinstname:Universidad Nacional de Trujilloinstacron:UNITRUspahttps://revistas.unitru.edu.pe/index.php/scientiaagrop/article/view/731/666https://revistas.unitru.edu.pe/index.php/scientiaagrop/article/view/731/1350Derechos de autor 2014 Scientia Agropecuariainfo:eu-repo/semantics/openAccessoai:ojs.revistas.unitru.edu.pe:article/7312017-03-07T15:21:26Z
score 13.361119
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