Analysis of Genetic Variability among thirty accessions of Andean Lupin (Lupinus mutabilis Sweet) using ISSR molecular markers

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In order to make the genetic variability analysis among thirty accessions of andean lupine (L. mutabilis Sweet) belonging to Agrarian Innovation National Institute (INIA) Seed Bank. DNA was extracted from 300 plants and we made bulks. We standardized amplification protocol of Inter Simple Sequence R...

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Detalles Bibliográficos
Autores: Chirinos-Arias, Michelle C., Jiménez, Jorge E., Vilca-Machaca, Lizbeth S.
Formato: artículo
Fecha de Publicación:2015
Institución:Universidad Nacional de Trujillo
Repositorio:Revistas - Universidad Nacional de Trujillo
Lenguaje:español
OAI Identifier:oai:ojs.revistas.unitru.edu.pe:article/808
Enlace del recurso:https://revistas.unitru.edu.pe/index.php/scientiaagrop/article/view/808
Nivel de acceso:acceso abierto
Materia:Lupinus mutabilis
bulks
genetic variability
ISSR
variabilidad genética
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spelling Analysis of Genetic Variability among thirty accessions of Andean Lupin (Lupinus mutabilis Sweet) using ISSR molecular markersAnálisis de la Variabilidad Genética entre treinta accesiones de tarwi (Lupinus mutabilis Sweet) usando marcadores moleculares ISSRChirinos-Arias, Michelle C.Jiménez, Jorge E.Vilca-Machaca, Lizbeth S.Lupinus mutabilisbulksgenetic variabilityISSRLupinus mutabilisbulksvariabilidad genéticaISSRIn order to make the genetic variability analysis among thirty accessions of andean lupine (L. mutabilis Sweet) belonging to Agrarian Innovation National Institute (INIA) Seed Bank. DNA was extracted from 300 plants and we made bulks. We standardized amplification protocol of Inter Simple Sequence Repeat (ISSR) primers, we chose the most polymorphic primers to run in acrylamide gel. We found 255 ISSR loci with 8 primers. It was found high genetic variability of the samples under study by ISSR markers. Also observed relatively high polymorphism for autogamous species such as andean lupine. Finally phenograms showed a relationship with the geographical location, possibly due to in situ gene flow due to the exchange or sale of seeds in markets near the collection area.Con el fin de realizar el análisis de variabilidad genética inter-accesión de treinta accesiones de tarwi (L. mutabilis Sweet) pertenecientes al Banco de Germoplasma del Instituto Nacional de Innovación Agraria (INIA). Se extrajo el ADN de 300 plantas, se construyeron bulks, se estandarizó el protocolo de amplificación de los marcadores moleculares Inter Simple Sequence Repeat (ISSR), de los cuales se eligió a los más polimórficos y nítidos para corrida en gel de acrilamida. Encontrándose 255 bandas con 8 iniciadores ISSR. El análisis de la variabilidad genética con estos iniciadores comprobó una alta variabilidad genética de las muestras en estudio. Observándose también un polimorfismo relativamente alto para una especie autógama como L. mutabilis. Finalmente los fenogramas mostraron una relación con la ubicación geográfica, posiblemente debido al flujo génico in situ debido al intercambio o venta de semillas en ferias o mercados aledaños a la zona de colecta.Universidad Nacional de Trujillo2015-03-31info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://revistas.unitru.edu.pe/index.php/scientiaagrop/article/view/808Scientia Agropecuaria; Vol. 6 Núm. 1 (2015): Enero - Marzo; 17-30Scientia Agropecuaria; Vol. 6 No. 1 (2015): January - March; 17-302306-67412077-9917reponame:Revistas - Universidad Nacional de Trujilloinstname:Universidad Nacional de Trujilloinstacron:UNITRUspahttps://revistas.unitru.edu.pe/index.php/scientiaagrop/article/view/808/736https://revistas.unitru.edu.pe/index.php/scientiaagrop/article/view/808/1356Derechos de autor 2015 Scientia Agropecuariainfo:eu-repo/semantics/openAccessoai:ojs.revistas.unitru.edu.pe:article/8082017-03-07T15:41:34Z
dc.title.none.fl_str_mv Analysis of Genetic Variability among thirty accessions of Andean Lupin (Lupinus mutabilis Sweet) using ISSR molecular markers
Análisis de la Variabilidad Genética entre treinta accesiones de tarwi (Lupinus mutabilis Sweet) usando marcadores moleculares ISSR
title Analysis of Genetic Variability among thirty accessions of Andean Lupin (Lupinus mutabilis Sweet) using ISSR molecular markers
spellingShingle Analysis of Genetic Variability among thirty accessions of Andean Lupin (Lupinus mutabilis Sweet) using ISSR molecular markers
Chirinos-Arias, Michelle C.
Lupinus mutabilis
bulks
genetic variability
ISSR
Lupinus mutabilis
bulks
variabilidad genética
ISSR
title_short Analysis of Genetic Variability among thirty accessions of Andean Lupin (Lupinus mutabilis Sweet) using ISSR molecular markers
title_full Analysis of Genetic Variability among thirty accessions of Andean Lupin (Lupinus mutabilis Sweet) using ISSR molecular markers
title_fullStr Analysis of Genetic Variability among thirty accessions of Andean Lupin (Lupinus mutabilis Sweet) using ISSR molecular markers
title_full_unstemmed Analysis of Genetic Variability among thirty accessions of Andean Lupin (Lupinus mutabilis Sweet) using ISSR molecular markers
title_sort Analysis of Genetic Variability among thirty accessions of Andean Lupin (Lupinus mutabilis Sweet) using ISSR molecular markers
dc.creator.none.fl_str_mv Chirinos-Arias, Michelle C.
Jiménez, Jorge E.
Vilca-Machaca, Lizbeth S.
author Chirinos-Arias, Michelle C.
author_facet Chirinos-Arias, Michelle C.
Jiménez, Jorge E.
Vilca-Machaca, Lizbeth S.
author_role author
author2 Jiménez, Jorge E.
Vilca-Machaca, Lizbeth S.
author2_role author
author
dc.subject.none.fl_str_mv Lupinus mutabilis
bulks
genetic variability
ISSR
Lupinus mutabilis
bulks
variabilidad genética
ISSR
topic Lupinus mutabilis
bulks
genetic variability
ISSR
Lupinus mutabilis
bulks
variabilidad genética
ISSR
description In order to make the genetic variability analysis among thirty accessions of andean lupine (L. mutabilis Sweet) belonging to Agrarian Innovation National Institute (INIA) Seed Bank. DNA was extracted from 300 plants and we made bulks. We standardized amplification protocol of Inter Simple Sequence Repeat (ISSR) primers, we chose the most polymorphic primers to run in acrylamide gel. We found 255 ISSR loci with 8 primers. It was found high genetic variability of the samples under study by ISSR markers. Also observed relatively high polymorphism for autogamous species such as andean lupine. Finally phenograms showed a relationship with the geographical location, possibly due to in situ gene flow due to the exchange or sale of seeds in markets near the collection area.
publishDate 2015
dc.date.none.fl_str_mv 2015-03-31
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://revistas.unitru.edu.pe/index.php/scientiaagrop/article/view/808
url https://revistas.unitru.edu.pe/index.php/scientiaagrop/article/view/808
dc.language.none.fl_str_mv spa
language spa
dc.relation.none.fl_str_mv https://revistas.unitru.edu.pe/index.php/scientiaagrop/article/view/808/736
https://revistas.unitru.edu.pe/index.php/scientiaagrop/article/view/808/1356
dc.rights.none.fl_str_mv Derechos de autor 2015 Scientia Agropecuaria
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Derechos de autor 2015 Scientia Agropecuaria
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Universidad Nacional de Trujillo
publisher.none.fl_str_mv Universidad Nacional de Trujillo
dc.source.none.fl_str_mv Scientia Agropecuaria; Vol. 6 Núm. 1 (2015): Enero - Marzo; 17-30
Scientia Agropecuaria; Vol. 6 No. 1 (2015): January - March; 17-30
2306-6741
2077-9917
reponame:Revistas - Universidad Nacional de Trujillo
instname:Universidad Nacional de Trujillo
instacron:UNITRU
instname_str Universidad Nacional de Trujillo
instacron_str UNITRU
institution UNITRU
reponame_str Revistas - Universidad Nacional de Trujillo
collection Revistas - Universidad Nacional de Trujillo
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 13.425424
Analysis of Genetic Variability among thirty accessions of Andean Lupin (Lupinus mutabilis Sweet) using ISSR molecular markers
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