Rhizospheric actinomycetes from organic crops of native potato (Solanum tuberosum): isolation, phenotypic characterization, molecular identification, and impact on biocontrol of Phytophthora infestans (Mont.) de Bary
Descripción del Articulo
Potato (Solanum tuberosum L.) is the fourth most consumed food crop in the world, whose production in Peru is diminished due to phytosanitary problems and high costs of chemical fertilizers. In the present work, 32 actinomycetes isolated from the rhizosphere of organic native potato crops collected...
Autores: | , , , |
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Formato: | artículo |
Fecha de Publicación: | 2020 |
Institución: | Universidad Nacional de Trujillo |
Repositorio: | Revistas - Universidad Nacional de Trujillo |
Lenguaje: | inglés |
OAI Identifier: | oai:ojs.revistas.unitru.edu.pe:article/2923 |
Enlace del recurso: | https://revistas.unitru.edu.pe/index.php/scientiaagrop/article/view/2923 |
Nivel de acceso: | acceso abierto |
Materia: | actinobacteria Phytophthora infestans native potato biocontrol of pests antagonism. |
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dc.title.none.fl_str_mv |
Rhizospheric actinomycetes from organic crops of native potato (Solanum tuberosum): isolation, phenotypic characterization, molecular identification, and impact on biocontrol of Phytophthora infestans (Mont.) de Bary Rhizospheric actinomycetes from organic crops of native potato (Solanum tuberosum): isolation, phenotypic characterization, molecular identification, and impact on biocontrol of Phytophthora infestans (Mont.) de Bary |
title |
Rhizospheric actinomycetes from organic crops of native potato (Solanum tuberosum): isolation, phenotypic characterization, molecular identification, and impact on biocontrol of Phytophthora infestans (Mont.) de Bary |
spellingShingle |
Rhizospheric actinomycetes from organic crops of native potato (Solanum tuberosum): isolation, phenotypic characterization, molecular identification, and impact on biocontrol of Phytophthora infestans (Mont.) de Bary Chumpitaz, Astrid actinobacteria Phytophthora infestans native potato biocontrol of pests antagonism. |
title_short |
Rhizospheric actinomycetes from organic crops of native potato (Solanum tuberosum): isolation, phenotypic characterization, molecular identification, and impact on biocontrol of Phytophthora infestans (Mont.) de Bary |
title_full |
Rhizospheric actinomycetes from organic crops of native potato (Solanum tuberosum): isolation, phenotypic characterization, molecular identification, and impact on biocontrol of Phytophthora infestans (Mont.) de Bary |
title_fullStr |
Rhizospheric actinomycetes from organic crops of native potato (Solanum tuberosum): isolation, phenotypic characterization, molecular identification, and impact on biocontrol of Phytophthora infestans (Mont.) de Bary |
title_full_unstemmed |
Rhizospheric actinomycetes from organic crops of native potato (Solanum tuberosum): isolation, phenotypic characterization, molecular identification, and impact on biocontrol of Phytophthora infestans (Mont.) de Bary |
title_sort |
Rhizospheric actinomycetes from organic crops of native potato (Solanum tuberosum): isolation, phenotypic characterization, molecular identification, and impact on biocontrol of Phytophthora infestans (Mont.) de Bary |
dc.creator.none.fl_str_mv |
Chumpitaz, Astrid Caro, Junior Cruz, Wilbert León, Jorge |
author |
Chumpitaz, Astrid |
author_facet |
Chumpitaz, Astrid Caro, Junior Cruz, Wilbert León, Jorge |
author_role |
author |
author2 |
Caro, Junior Cruz, Wilbert León, Jorge |
author2_role |
author author author |
dc.subject.none.fl_str_mv |
actinobacteria Phytophthora infestans native potato biocontrol of pests antagonism. |
topic |
actinobacteria Phytophthora infestans native potato biocontrol of pests antagonism. |
description |
Potato (Solanum tuberosum L.) is the fourth most consumed food crop in the world, whose production in Peru is diminished due to phytosanitary problems and high costs of chemical fertilizers. In the present work, 32 actinomycetes isolated from the rhizosphere of organic native potato crops collected in the town of Cabana, Lucanas, Ayacucho, were characterized phenotypically and evaluated for their in vitro antagonistic capacity against Oomycete phytopathogen Phytophthora infestans. The characterization tests showed that 97% of the actinomycetes were able to assimilate glucose, sucrose, and mannitol; as well as producing extracellular enzymes like amylases (100%) and cellulases (50%). Furthermore, the growth in laboratory culture was better in the range of pH 5.5-8.5 and temperature 28-30 °C. From the tests of antagonism in oat agar (71.9%) and rye agar (31.2%), three strains were selected according to the native potato variety were selected as CAB10-J2 (Ccompis), CAB9-CA4 (Cuchipa-akan) and CAB5-F5 (Futis) with pathogen inhibition rates of 80.05, 77.47 and 37.5% respectively. The strains were identified by molecular tests as members of the genus Streptomyces and owners of polyketide synthase (PKS) genes. It is concluded that the rhizospheric actinomycetes of potato are producers of bioactive compounds capable of remarkably inhibiting the pathogen Phytophthora infestans, being able to be considered candidates in biological control programs of the "potato blight". |
publishDate |
2020 |
dc.date.none.fl_str_mv |
2020-06-08 |
dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.none.fl_str_mv |
https://revistas.unitru.edu.pe/index.php/scientiaagrop/article/view/2923 |
url |
https://revistas.unitru.edu.pe/index.php/scientiaagrop/article/view/2923 |
dc.language.none.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
https://revistas.unitru.edu.pe/index.php/scientiaagrop/article/view/2923/3089 https://revistas.unitru.edu.pe/index.php/scientiaagrop/article/view/2923/3267 |
dc.rights.none.fl_str_mv |
Derechos de autor 2020 Scientia Agropecuaria info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
Derechos de autor 2020 Scientia Agropecuaria |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Universidad Nacional de Trujillo |
publisher.none.fl_str_mv |
Universidad Nacional de Trujillo |
dc.source.none.fl_str_mv |
Scientia Agropecuaria; Vol. 11 Núm. 2 (2020): Abril - Junio; 223-231 Scientia Agropecuaria; Vol. 11 No. 2 (2020): Abril - Junio; 223-231 2306-6741 2077-9917 reponame:Revistas - Universidad Nacional de Trujillo instname:Universidad Nacional de Trujillo instacron:UNITRU |
instname_str |
Universidad Nacional de Trujillo |
instacron_str |
UNITRU |
institution |
UNITRU |
reponame_str |
Revistas - Universidad Nacional de Trujillo |
collection |
Revistas - Universidad Nacional de Trujillo |
repository.name.fl_str_mv |
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repository.mail.fl_str_mv |
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1845886911423447040 |
spelling |
Rhizospheric actinomycetes from organic crops of native potato (Solanum tuberosum): isolation, phenotypic characterization, molecular identification, and impact on biocontrol of Phytophthora infestans (Mont.) de BaryRhizospheric actinomycetes from organic crops of native potato (Solanum tuberosum): isolation, phenotypic characterization, molecular identification, and impact on biocontrol of Phytophthora infestans (Mont.) de BaryChumpitaz, AstridCaro, JuniorCruz, WilbertLeón, JorgeactinobacteriaPhytophthora infestansnative potatobiocontrol of pestsantagonism.Potato (Solanum tuberosum L.) is the fourth most consumed food crop in the world, whose production in Peru is diminished due to phytosanitary problems and high costs of chemical fertilizers. In the present work, 32 actinomycetes isolated from the rhizosphere of organic native potato crops collected in the town of Cabana, Lucanas, Ayacucho, were characterized phenotypically and evaluated for their in vitro antagonistic capacity against Oomycete phytopathogen Phytophthora infestans. The characterization tests showed that 97% of the actinomycetes were able to assimilate glucose, sucrose, and mannitol; as well as producing extracellular enzymes like amylases (100%) and cellulases (50%). Furthermore, the growth in laboratory culture was better in the range of pH 5.5-8.5 and temperature 28-30 °C. From the tests of antagonism in oat agar (71.9%) and rye agar (31.2%), three strains were selected according to the native potato variety were selected as CAB10-J2 (Ccompis), CAB9-CA4 (Cuchipa-akan) and CAB5-F5 (Futis) with pathogen inhibition rates of 80.05, 77.47 and 37.5% respectively. The strains were identified by molecular tests as members of the genus Streptomyces and owners of polyketide synthase (PKS) genes. It is concluded that the rhizospheric actinomycetes of potato are producers of bioactive compounds capable of remarkably inhibiting the pathogen Phytophthora infestans, being able to be considered candidates in biological control programs of the "potato blight".Potato (Solanum tuberosum L.) is the fourth most consumed food crop in the world, whose production in Peru is diminished due to phytosanitary problems and high costs of chemical fertilizers. In the present work, 32 actinomycetes isolated from the rhizosphere of organic native potato crops collected in the town of Cabana, Lucanas, Ayacucho, were characterized phenotypically and evaluated for their in vitro antagonistic capacity against Oomycete phytopathogen Phytophthora infestans. The characterization tests showed that 97% of the actinomycetes were able to assimilate glucose, sucrose, and mannitol; as well as producing extracellular enzymes like amylases (100%) and cellulases (50%). Furthermore, the growth in laboratory culture was better in the range of pH 5.5-8.5 and temperature 28-30 °C. From the tests of antagonism in oat agar (71.9%) and rye agar (31.2%), three strains were selected according to the native potato variety were selected as CAB10-J2 (Ccompis), CAB9-CA4 (Cuchipa-akan) and CAB5-F5 (Futis) with pathogen inhibition rates of 80.05, 77.47 and 37.5% respectively. The strains were identified by molecular tests as members of the genus Streptomyces and owners of polyketide synthase (PKS) genes. It is concluded that the rhizospheric actinomycetes of potato are producers of bioactive compounds capable of remarkably inhibiting the pathogen Phytophthora infestans, being able to be considered candidates in biological control programs of the "potato blight".Universidad Nacional de Trujillo2020-06-08info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://revistas.unitru.edu.pe/index.php/scientiaagrop/article/view/2923Scientia Agropecuaria; Vol. 11 Núm. 2 (2020): Abril - Junio; 223-231Scientia Agropecuaria; Vol. 11 No. 2 (2020): Abril - Junio; 223-2312306-67412077-9917reponame:Revistas - Universidad Nacional de Trujilloinstname:Universidad Nacional de Trujilloinstacron:UNITRUenghttps://revistas.unitru.edu.pe/index.php/scientiaagrop/article/view/2923/3089https://revistas.unitru.edu.pe/index.php/scientiaagrop/article/view/2923/3267Derechos de autor 2020 Scientia Agropecuariainfo:eu-repo/semantics/openAccessoai:ojs.revistas.unitru.edu.pe:article/29232021-07-20T17:12:09Z |
score |
13.037861 |
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La información contenida en este registro es de entera responsabilidad de la institución que gestiona el repositorio institucional donde esta contenido este documento o set de datos. El CONCYTEC no se hace responsable por los contenidos (publicaciones y/o datos) accesibles a través del Repositorio Nacional Digital de Ciencia, Tecnología e Innovación de Acceso Abierto (ALICIA).
La información contenida en este registro es de entera responsabilidad de la institución que gestiona el repositorio institucional donde esta contenido este documento o set de datos. El CONCYTEC no se hace responsable por los contenidos (publicaciones y/o datos) accesibles a través del Repositorio Nacional Digital de Ciencia, Tecnología e Innovación de Acceso Abierto (ALICIA).