Rhizospheric actinomycetes from organic crops of native potato (Solanum tuberosum): isolation, phenotypic characterization, molecular identification, and impact on biocontrol of Phytophthora infestans (Mont.) de Bary

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Potato (Solanum tuberosum L.) is the fourth most consumed food crop in the world, whose production in Peru is diminished due to phytosanitary problems and high costs of chemical fertilizers. In the present work, 32 actinomycetes isolated from the rhizosphere of organic native potato crops collected...

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Detalles Bibliográficos
Autores: Chumpitaz, Astrid, Caro, Junior, Cruz, Wilbert, León, Jorge
Formato: artículo
Fecha de Publicación:2020
Institución:Universidad Nacional de Trujillo
Repositorio:Revistas - Universidad Nacional de Trujillo
Lenguaje:inglés
OAI Identifier:oai:ojs.revistas.unitru.edu.pe:article/2923
Enlace del recurso:https://revistas.unitru.edu.pe/index.php/scientiaagrop/article/view/2923
Nivel de acceso:acceso abierto
Materia:actinobacteria
Phytophthora infestans
native potato
biocontrol of pests
antagonism.
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dc.title.none.fl_str_mv Rhizospheric actinomycetes from organic crops of native potato (Solanum tuberosum): isolation, phenotypic characterization, molecular identification, and impact on biocontrol of Phytophthora infestans (Mont.) de Bary
Rhizospheric actinomycetes from organic crops of native potato (Solanum tuberosum): isolation, phenotypic characterization, molecular identification, and impact on biocontrol of Phytophthora infestans (Mont.) de Bary
title Rhizospheric actinomycetes from organic crops of native potato (Solanum tuberosum): isolation, phenotypic characterization, molecular identification, and impact on biocontrol of Phytophthora infestans (Mont.) de Bary
spellingShingle Rhizospheric actinomycetes from organic crops of native potato (Solanum tuberosum): isolation, phenotypic characterization, molecular identification, and impact on biocontrol of Phytophthora infestans (Mont.) de Bary
Chumpitaz, Astrid
actinobacteria
Phytophthora infestans
native potato
biocontrol of pests
antagonism.
title_short Rhizospheric actinomycetes from organic crops of native potato (Solanum tuberosum): isolation, phenotypic characterization, molecular identification, and impact on biocontrol of Phytophthora infestans (Mont.) de Bary
title_full Rhizospheric actinomycetes from organic crops of native potato (Solanum tuberosum): isolation, phenotypic characterization, molecular identification, and impact on biocontrol of Phytophthora infestans (Mont.) de Bary
title_fullStr Rhizospheric actinomycetes from organic crops of native potato (Solanum tuberosum): isolation, phenotypic characterization, molecular identification, and impact on biocontrol of Phytophthora infestans (Mont.) de Bary
title_full_unstemmed Rhizospheric actinomycetes from organic crops of native potato (Solanum tuberosum): isolation, phenotypic characterization, molecular identification, and impact on biocontrol of Phytophthora infestans (Mont.) de Bary
title_sort Rhizospheric actinomycetes from organic crops of native potato (Solanum tuberosum): isolation, phenotypic characterization, molecular identification, and impact on biocontrol of Phytophthora infestans (Mont.) de Bary
dc.creator.none.fl_str_mv Chumpitaz, Astrid
Caro, Junior
Cruz, Wilbert
León, Jorge
author Chumpitaz, Astrid
author_facet Chumpitaz, Astrid
Caro, Junior
Cruz, Wilbert
León, Jorge
author_role author
author2 Caro, Junior
Cruz, Wilbert
León, Jorge
author2_role author
author
author
dc.subject.none.fl_str_mv actinobacteria
Phytophthora infestans
native potato
biocontrol of pests
antagonism.
topic actinobacteria
Phytophthora infestans
native potato
biocontrol of pests
antagonism.
description Potato (Solanum tuberosum L.) is the fourth most consumed food crop in the world, whose production in Peru is diminished due to phytosanitary problems and high costs of chemical fertilizers. In the present work, 32 actinomycetes isolated from the rhizosphere of organic native potato crops collected in the town of Cabana, Lucanas, Ayacucho, were characterized phenotypically and evaluated for their in vitro antagonistic capacity against Oomycete phytopathogen Phytophthora infestans. The characterization tests showed that 97% of the actinomycetes were able to assimilate glucose, sucrose, and mannitol; as well as producing extracellular enzymes like amylases (100%) and cellulases (50%). Furthermore, the growth in laboratory culture was better in the range of pH 5.5-8.5 and temperature 28-30 °C. From the tests of antagonism in oat agar (71.9%) and rye agar (31.2%), three strains were selected according to the native potato variety were selected as CAB10-J2 (Ccompis), CAB9-CA4 (Cuchipa-akan) and CAB5-F5 (Futis) with pathogen inhibition rates of 80.05, 77.47 and 37.5% respectively. The strains were identified by molecular tests as members of the genus Streptomyces and owners of polyketide synthase (PKS) genes. It is concluded that the rhizospheric actinomycetes of potato are producers of bioactive compounds capable of remarkably inhibiting the pathogen Phytophthora infestans, being able to be considered candidates in biological control programs of the "potato blight".
publishDate 2020
dc.date.none.fl_str_mv 2020-06-08
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://revistas.unitru.edu.pe/index.php/scientiaagrop/article/view/2923
url https://revistas.unitru.edu.pe/index.php/scientiaagrop/article/view/2923
dc.language.none.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv https://revistas.unitru.edu.pe/index.php/scientiaagrop/article/view/2923/3089
https://revistas.unitru.edu.pe/index.php/scientiaagrop/article/view/2923/3267
dc.rights.none.fl_str_mv Derechos de autor 2020 Scientia Agropecuaria
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Derechos de autor 2020 Scientia Agropecuaria
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Universidad Nacional de Trujillo
publisher.none.fl_str_mv Universidad Nacional de Trujillo
dc.source.none.fl_str_mv Scientia Agropecuaria; Vol. 11 Núm. 2 (2020): Abril - Junio; 223-231
Scientia Agropecuaria; Vol. 11 No. 2 (2020): Abril - Junio; 223-231
2306-6741
2077-9917
reponame:Revistas - Universidad Nacional de Trujillo
instname:Universidad Nacional de Trujillo
instacron:UNITRU
instname_str Universidad Nacional de Trujillo
instacron_str UNITRU
institution UNITRU
reponame_str Revistas - Universidad Nacional de Trujillo
collection Revistas - Universidad Nacional de Trujillo
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spelling Rhizospheric actinomycetes from organic crops of native potato (Solanum tuberosum): isolation, phenotypic characterization, molecular identification, and impact on biocontrol of Phytophthora infestans (Mont.) de BaryRhizospheric actinomycetes from organic crops of native potato (Solanum tuberosum): isolation, phenotypic characterization, molecular identification, and impact on biocontrol of Phytophthora infestans (Mont.) de BaryChumpitaz, AstridCaro, JuniorCruz, WilbertLeón, JorgeactinobacteriaPhytophthora infestansnative potatobiocontrol of pestsantagonism.Potato (Solanum tuberosum L.) is the fourth most consumed food crop in the world, whose production in Peru is diminished due to phytosanitary problems and high costs of chemical fertilizers. In the present work, 32 actinomycetes isolated from the rhizosphere of organic native potato crops collected in the town of Cabana, Lucanas, Ayacucho, were characterized phenotypically and evaluated for their in vitro antagonistic capacity against Oomycete phytopathogen Phytophthora infestans. The characterization tests showed that 97% of the actinomycetes were able to assimilate glucose, sucrose, and mannitol; as well as producing extracellular enzymes like amylases (100%) and cellulases (50%). Furthermore, the growth in laboratory culture was better in the range of pH 5.5-8.5 and temperature 28-30 °C. From the tests of antagonism in oat agar (71.9%) and rye agar (31.2%), three strains were selected according to the native potato variety were selected as CAB10-J2 (Ccompis), CAB9-CA4 (Cuchipa-akan) and CAB5-F5 (Futis) with pathogen inhibition rates of 80.05, 77.47 and 37.5% respectively. The strains were identified by molecular tests as members of the genus Streptomyces and owners of polyketide synthase (PKS) genes. It is concluded that the rhizospheric actinomycetes of potato are producers of bioactive compounds capable of remarkably inhibiting the pathogen Phytophthora infestans, being able to be considered candidates in biological control programs of the "potato blight".Potato (Solanum tuberosum L.) is the fourth most consumed food crop in the world, whose production in Peru is diminished due to phytosanitary problems and high costs of chemical fertilizers. In the present work, 32 actinomycetes isolated from the rhizosphere of organic native potato crops collected in the town of Cabana, Lucanas, Ayacucho, were characterized phenotypically and evaluated for their in vitro antagonistic capacity against Oomycete phytopathogen Phytophthora infestans. The characterization tests showed that 97% of the actinomycetes were able to assimilate glucose, sucrose, and mannitol; as well as producing extracellular enzymes like amylases (100%) and cellulases (50%). Furthermore, the growth in laboratory culture was better in the range of pH 5.5-8.5 and temperature 28-30 °C. From the tests of antagonism in oat agar (71.9%) and rye agar (31.2%), three strains were selected according to the native potato variety were selected as CAB10-J2 (Ccompis), CAB9-CA4 (Cuchipa-akan) and CAB5-F5 (Futis) with pathogen inhibition rates of 80.05, 77.47 and 37.5% respectively. The strains were identified by molecular tests as members of the genus Streptomyces and owners of polyketide synthase (PKS) genes. It is concluded that the rhizospheric actinomycetes of potato are producers of bioactive compounds capable of remarkably inhibiting the pathogen Phytophthora infestans, being able to be considered candidates in biological control programs of the "potato blight".Universidad Nacional de Trujillo2020-06-08info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://revistas.unitru.edu.pe/index.php/scientiaagrop/article/view/2923Scientia Agropecuaria; Vol. 11 Núm. 2 (2020): Abril - Junio; 223-231Scientia Agropecuaria; Vol. 11 No. 2 (2020): Abril - Junio; 223-2312306-67412077-9917reponame:Revistas - Universidad Nacional de Trujilloinstname:Universidad Nacional de Trujilloinstacron:UNITRUenghttps://revistas.unitru.edu.pe/index.php/scientiaagrop/article/view/2923/3089https://revistas.unitru.edu.pe/index.php/scientiaagrop/article/view/2923/3267Derechos de autor 2020 Scientia Agropecuariainfo:eu-repo/semantics/openAccessoai:ojs.revistas.unitru.edu.pe:article/29232021-07-20T17:12:09Z
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