Polyhydroxyalcanoates of strains of Azospirillum spp. isolated of roots of Lycopersicon esculentum Mill. “tomato” and Oryza sativa L. “rice” in Lambayeque

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In this work was determined the concentration of polyhydroxyalkanoates (PHAs) of Azospirillum strains isolated from roots of Lycopersicon esculentum Mill "tomato" and Oryza sativa L. "rice" as an alternative to accumulation of petroleum-based plastics. Previously disinfected root...

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Detalles Bibliográficos
Autores: Baca, Katty, Sánchez, Melgy, Carreño, Carmen, Mendoza, Gilmar
Formato: artículo
Fecha de Publicación:2010
Institución:Universidad Nacional de Trujillo
Repositorio:Revistas - Universidad Nacional de Trujillo
Lenguaje:español
OAI Identifier:oai:ojs.revistas.unitru.edu.pe:article/41
Enlace del recurso:https://revistas.unitru.edu.pe/index.php/scientiaagrop/article/view/41
Nivel de acceso:acceso abierto
Materia:Azospirillum
PHAs
Lycopersicon esculentum Mill
Oryza sativa L
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spelling Polyhydroxyalcanoates of strains of Azospirillum spp. isolated of roots of Lycopersicon esculentum Mill. “tomato” and Oryza sativa L. “rice” in LambayequePolihidroxialcanoatos de cepas de Azospirillum spp. aisladas de raíces de Lycopersicon esculentum Mill. “tomate” y Oryza sativa L. “arroz” en LambayequeBaca, KattySánchez, MelgyCarreño, CarmenMendoza, GilmarAzospirillumPHAsLycopersicon esculentum MillOryza sativa LAzospirillumPHAsLycopersicon esculentum MillOryza sativa LIn this work was determined the concentration of polyhydroxyalkanoates (PHAs) of Azospirillum strains isolated from roots of Lycopersicon esculentum Mill "tomato" and Oryza sativa L. "rice" as an alternative to accumulation of petroleum-based plastics. Previously disinfected root were plated in Nfb semisolid medium where nitrogen-fixing bacteria were recognized by a whitish film on the surface and turn from green to blue. The genus Azospirillum was identified in Congo red agar medium, obtained 96 isolates of A. lipoferum and A. brasilense on tomato and rice. Batch fermentation was performed with broth Azotobacter modified feeding a saturated solution of malic acid every 12 hours and were stained with Sudan Black B. Strains were selected with the greatest number of PHAs granules (in tomato, 18 of A. lipoferum and 2 of A. brasilense; in rice, 10 of A. lipoferum and 10 of A. brasilense ) and quantified the biomass and PHAs. PHAs concentration reached 0.661 gL-1 in A. lipoferum KM(T)-73 and 0.738 gL-1 in A. brasilense KM(T)-19, both isolated from tomato. Strains of A. lipoferum and A. brasilense isolated from tomato reached a higher concentration of biomass and PHAs against the strains of rice.En este trabajo se determinó la concentración de polihidroxialcanoatos (PHAs) de cepas de Azospirillum aisladas de raíces de Lycopersicon esculentum Mill. “tomate” y Oryza sativa L. “arroz”, como una alternativa ante la acumulación de plásticos derivados del petróleo. Raíces previamente desinfectadas se sembraron en medio Nfb semisólido, donde las bacterias fijadoras de nitrógeno se reconocieron por una película blanquecina bajo la superficie y el viraje del indicador al azul. El género Azospirillum se identificó en medio rojo de Congo, obteniéndose 96 cepas de A. lipoferum y A. brasilense en tomate y arroz. Se realizó una fermentación discontinua con caldo Azotobacter modificado, alimentando con una solución saturada de ácido málico cada 12 horas y se realizaron tinciones con Sudán Negro B. Se seleccionaron las cepas con mayor número de gránulos de PHAs (en tomate, 18 de A. lipoferum y 2 de A. brasilense y en arroz, 10 de A. lipoferum y 10 de A. brasilense) y se cuantificó la biomasa y PHAs. La concentración de PHAs alcanzó 0.661 gL-1 en A. lipoferum KM(T)-73 y 0.738 gL-1 en A. brasilense KM(T)-19. Las cepas de A. lipoferum y A. brasilense aisladas de tomate alcanzaron una mayor concentración de biomasa y PHAs frente a las cepas aisladas de arroz.Universidad Nacional de Trujillo2010-07-25info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://revistas.unitru.edu.pe/index.php/scientiaagrop/article/view/41Scientia Agropecuaria; Vol. 1 Núm. 3 y 4 (2010): Julio - Diciembre; 213-224Scientia Agropecuaria; Vol. 1 No. 3 y 4 (2010): July - December; 213-2242306-67412077-9917reponame:Revistas - Universidad Nacional de Trujilloinstname:Universidad Nacional de Trujilloinstacron:UNITRUspahttps://revistas.unitru.edu.pe/index.php/scientiaagrop/article/view/41/46Derechos de autor 2010 Scientia Agropecuariainfo:eu-repo/semantics/openAccessoai:ojs.revistas.unitru.edu.pe:article/412017-04-18T10:05:33Z
dc.title.none.fl_str_mv Polyhydroxyalcanoates of strains of Azospirillum spp. isolated of roots of Lycopersicon esculentum Mill. “tomato” and Oryza sativa L. “rice” in Lambayeque
Polihidroxialcanoatos de cepas de Azospirillum spp. aisladas de raíces de Lycopersicon esculentum Mill. “tomate” y Oryza sativa L. “arroz” en Lambayeque
title Polyhydroxyalcanoates of strains of Azospirillum spp. isolated of roots of Lycopersicon esculentum Mill. “tomato” and Oryza sativa L. “rice” in Lambayeque
spellingShingle Polyhydroxyalcanoates of strains of Azospirillum spp. isolated of roots of Lycopersicon esculentum Mill. “tomato” and Oryza sativa L. “rice” in Lambayeque
Baca, Katty
Azospirillum
PHAs
Lycopersicon esculentum Mill
Oryza sativa L
Azospirillum
PHAs
Lycopersicon esculentum Mill
Oryza sativa L
title_short Polyhydroxyalcanoates of strains of Azospirillum spp. isolated of roots of Lycopersicon esculentum Mill. “tomato” and Oryza sativa L. “rice” in Lambayeque
title_full Polyhydroxyalcanoates of strains of Azospirillum spp. isolated of roots of Lycopersicon esculentum Mill. “tomato” and Oryza sativa L. “rice” in Lambayeque
title_fullStr Polyhydroxyalcanoates of strains of Azospirillum spp. isolated of roots of Lycopersicon esculentum Mill. “tomato” and Oryza sativa L. “rice” in Lambayeque
title_full_unstemmed Polyhydroxyalcanoates of strains of Azospirillum spp. isolated of roots of Lycopersicon esculentum Mill. “tomato” and Oryza sativa L. “rice” in Lambayeque
title_sort Polyhydroxyalcanoates of strains of Azospirillum spp. isolated of roots of Lycopersicon esculentum Mill. “tomato” and Oryza sativa L. “rice” in Lambayeque
dc.creator.none.fl_str_mv Baca, Katty
Sánchez, Melgy
Carreño, Carmen
Mendoza, Gilmar
author Baca, Katty
author_facet Baca, Katty
Sánchez, Melgy
Carreño, Carmen
Mendoza, Gilmar
author_role author
author2 Sánchez, Melgy
Carreño, Carmen
Mendoza, Gilmar
author2_role author
author
author
dc.subject.none.fl_str_mv Azospirillum
PHAs
Lycopersicon esculentum Mill
Oryza sativa L
Azospirillum
PHAs
Lycopersicon esculentum Mill
Oryza sativa L
topic Azospirillum
PHAs
Lycopersicon esculentum Mill
Oryza sativa L
Azospirillum
PHAs
Lycopersicon esculentum Mill
Oryza sativa L
description In this work was determined the concentration of polyhydroxyalkanoates (PHAs) of Azospirillum strains isolated from roots of Lycopersicon esculentum Mill "tomato" and Oryza sativa L. "rice" as an alternative to accumulation of petroleum-based plastics. Previously disinfected root were plated in Nfb semisolid medium where nitrogen-fixing bacteria were recognized by a whitish film on the surface and turn from green to blue. The genus Azospirillum was identified in Congo red agar medium, obtained 96 isolates of A. lipoferum and A. brasilense on tomato and rice. Batch fermentation was performed with broth Azotobacter modified feeding a saturated solution of malic acid every 12 hours and were stained with Sudan Black B. Strains were selected with the greatest number of PHAs granules (in tomato, 18 of A. lipoferum and 2 of A. brasilense; in rice, 10 of A. lipoferum and 10 of A. brasilense ) and quantified the biomass and PHAs. PHAs concentration reached 0.661 gL-1 in A. lipoferum KM(T)-73 and 0.738 gL-1 in A. brasilense KM(T)-19, both isolated from tomato. Strains of A. lipoferum and A. brasilense isolated from tomato reached a higher concentration of biomass and PHAs against the strains of rice.
publishDate 2010
dc.date.none.fl_str_mv 2010-07-25
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://revistas.unitru.edu.pe/index.php/scientiaagrop/article/view/41
url https://revistas.unitru.edu.pe/index.php/scientiaagrop/article/view/41
dc.language.none.fl_str_mv spa
language spa
dc.relation.none.fl_str_mv https://revistas.unitru.edu.pe/index.php/scientiaagrop/article/view/41/46
dc.rights.none.fl_str_mv Derechos de autor 2010 Scientia Agropecuaria
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Derechos de autor 2010 Scientia Agropecuaria
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Universidad Nacional de Trujillo
publisher.none.fl_str_mv Universidad Nacional de Trujillo
dc.source.none.fl_str_mv Scientia Agropecuaria; Vol. 1 Núm. 3 y 4 (2010): Julio - Diciembre; 213-224
Scientia Agropecuaria; Vol. 1 No. 3 y 4 (2010): July - December; 213-224
2306-6741
2077-9917
reponame:Revistas - Universidad Nacional de Trujillo
instname:Universidad Nacional de Trujillo
instacron:UNITRU
instname_str Universidad Nacional de Trujillo
instacron_str UNITRU
institution UNITRU
reponame_str Revistas - Universidad Nacional de Trujillo
collection Revistas - Universidad Nacional de Trujillo
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 13.457506
Polyhydroxyalcanoates of strains of Azospirillum spp. isolated of roots of Lycopersicon esculentum Mill. “tomato” and Oryza sativa L. “rice” in Lambayeque
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