PURIFICATION AND SOME BIOCHEMICAL AND MOLECULAR PROPERTIES OF AN NEW NOT MYOTOXIC PHOSPHOLIPASE A2 FROM Bothrops atrox SNAKE VENOM

• Autores: Quispe, Edwin, Torrejón, Daniel, Bautista, Lorgio, Sandoval, Gustavo, Rodríguez, Edith, Lazo, Fanny, Vivas Ruiz, Dan, Yarlequé, Armando
• Formato: artículo
• Fecha de Publicación: 2019
• Institución: Sociedad Química del Perú
• Repositorio: Revista de la Sociedad Química del Perú
• Lenguaje: español
• OAI Identifier: oai:rsqp.revistas.sqperu.org.pe:article/263
• Enlace del recurso: http://revistas.sqperu.org.pe/index.php/revistasqperu/article/view/263
• Nivel de acceso: acceso abierto
• Materia: Bothrops atrox; venom; purification; acidic phospholipase A2; myotoxicity; veneno; purificación; fosfolipasa A2 ácida; miotoxicidad

Phospholipases A2 (PLA2 ) from snake venom are enzymes with highly variety of biological effects, due to their different isoforms and some may be myotoxins. This research aimed was to purify, characterize and evaluate the myotoxic activity of an isoform of acid PLA2 (BaPer-PLA2a). For purification, in DEAE Sephadex-A50, Sephadex-G75 and an automated medium pressure system-NGC were used. BaPer-PLA2a had a specific activity of 34.1 U/ mg and a MW of ~ 14.5 kDa by SDS-PAGE, non-reducing conditions. From venom were obtained total RNA, to synthesis of cDNA and an amplified of ~ 480 bp. A mature protein of 124 amino acids was deduced from the cDNA sequence with a pI (4.41), being an acidic isoform, likewise presented primary structure with conserved regions and residues His48, Asp49 and Tyr52 identified in the catalytic center. Additionally, the structural theoretical model has an identity greater than 70 % with other acidic PLA2. Finally, BaPer-PLA2a did not have myotoxic activity, however, when combined with the basic PLA2 isoform increased the last 's myotoxic activity in 21.58 %.