Comparison of two isothermal methods for SARS-COV-2 screening in Callao, Peru

• Autores: Cassa-Loaiza, William, Vásquez-Velásquez, Cinthya, Gonzales, Gustavo F., Velasquez-Medrano, Carol, Fernandez-Delgado, Kevin, Alvarez-Huambachano, Keyla, Gamboa-Serpa, Henry, Fano-Sizgorich, Diego
• Formato: artículo
• Fecha de Publicación: 2023
• Institución: Colegio Médico del Perú
• Repositorio: Acta Médica Peruana
• Lenguaje: inglés
• OAI Identifier: oai:ojs.pkp.sfu.ca:article/2475
• Enlace del recurso: https://amp.cmp.org.pe/index.php/AMP/article/view/2475
• Nivel de acceso: acceso abierto
• Materia: SARS-CoV-2; Reverse Transcriptase Polymerase Chain Reaction; Molecular Diagnostic Techniques

Objective: To compare the isothermal molecular screening techniques CPA and RT-LAMP, against the gold standard test, reverse transcription quantitative polymerase chain reaction (RT-qPCR), and to determine its agreement. Materials and Methods: Paired comparative case-control study. For the evaluation of the CPA method, 70 cases and 130 controls were used, while for RT-LAMP, 30 cases and 70 controls were used. The sensitivity and specificity of both tests were calculated. Subsequently, the bias-corrected Kappa index was calculated by resampling. Results: Both techniques have adequate and equivalent values of sensitivity (RT-LAMP: 82.8%, CPA: 83%) and specificity (RT-LAMP and CPA: 91.5%), as well as a high concordance (88%), and Kappa-index (0.72). Conclusion: Both isothermal molecular screening techniques are suitable for SARS-CoV-2 screening, with a similar sensitivity and specificity.