Assessment of specific cell-mediated immunity to SARS-CoV-2: techniques for its measurement

Descripción del Articulo

Understanding the immune response to infection or vaccination against SARS-CoV-2 has become a key objective for the understanding of the disease, its progression, and protection against future infections or possible new variants that may emerge of the virus. The aim of the present review is to focus...

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Detalles Bibliográficos
Autores: Enciso-Benavides, Javier, Garcia-Paita, Marlon, Alvarez-Vega, Santiago, Tapia-Rojas, Salyoc, Mayanga-Herrera, Ana, Pons, Maria J.
Formato: artículo
Fecha de Publicación:2026
Institución:Cuerpo Médico Hospital Nacional Almanzor Aguinaga Asenjo
Repositorio:Revista del Cuerpo Médico Hospital Nacional Almanzor Aguinaga Asenjo
Lenguaje:español
OAI Identifier:oai:cmhnaaa_ojs_cmhnaaa.cmhnaaa.org.pe:article/2762
Enlace del recurso:https://cmhnaaa.org.pe/ojs/index.php/rcmhnaaa/article/view/2762
Nivel de acceso:acceso abierto
Materia:Inmunidad adaptativa
linfocitos T
SARS-CoV-2
respuesta inmunológica
Adaptive Immunity
T Cells
Immunological response
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spelling Assessment of specific cell-mediated immunity to SARS-CoV-2: techniques for its measurementEvaluación de la inmunidad celular específica frente al SARS-CoV-2: técnicas para su mediciónEnciso-Benavides, Javier Garcia-Paita, MarlonAlvarez-Vega, Santiago Tapia-Rojas, Salyoc Mayanga-Herrera, AnaPons, Maria J.Inmunidad adaptativa linfocitos TSARS-CoV-2respuesta inmunológicaAdaptive ImmunityT CellsSARS-CoV-2Immunological response Understanding the immune response to infection or vaccination against SARS-CoV-2 has become a key objective for the understanding of the disease, its progression, and protection against future infections or possible new variants that may emerge of the virus. The aim of the present review is to focus on the relevance of the cellular immune response and to summarize the different methodologies described for the study of this memory T-cell-related response, either after exposure to the virus or vaccination against SARS-CoV-2. For this purpose, cell proliferation assays (CFSE, 3H-thymidine), cell typing methods (flow cytometry), immunological assays based on the measurement of cytokines (ELISA, ELISPOT, suspension multiplex) and analysis of genes related to cellular immunity by molecular techniques (RT-qPCR or qPCR and sequencing) have been included.Comprender la respuesta inmunitaria frente a la infección o vacunación contra SARS-CoV-2 se ha convertido en un objetivo clave para entender la enfermedad, su progresión, y protección frente a futuras infecciones o posibles nuevas variantes que puedan surgir del virus. El objetivo de la presente revisión es destacar la relevancia de la respuesta inmunológica celular y resumir las diferentes metodologías descritas para el estudio de esta respuesta mediada por células T, de memoria, tanto tras la exposición al virus como de la vacunación frente al SARS-CoV-2. Para ello, se incluyen los ensayos de proliferación celular (CFSE, 3H- timidina), métodos de tipificación celular (citometría de flujo), los ensayos inmunológicos basados en la medición de citocinas (ELISA, ELISPOT, Multiplexado en suspensión) y el análisis de genes relacionados con la inmunidad celular mediante técnicas moleculares (RT-qPCR o qPCR y secuenciación). Cuerpo Médico del Hospital Nacional Almanzor Aguinaga Asenjo2026-01-29info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontextoapplication/pdfhttps://cmhnaaa.org.pe/ojs/index.php/rcmhnaaa/article/view/276210.35434/rcmhnaaa.2025.184.2762Revista del Cuerpo Médico Hospital Nacional Almanzor Aguinaga Asenjo; Vol. 18 No. 4 (2025): Early PublicationRevista del Cuerpo Médico Hospital Nacional Almanzor Aguinaga Asenjo; Vol. 18 Núm. 4 (2025): Publicación Anticipada2227-47312225-510910.35434/rcmhnaaa.2025.184reponame:Revista del Cuerpo Médico Hospital Nacional Almanzor Aguinaga Asenjoinstname:Cuerpo Médico Hospital Nacional Almanzor Aguinaga Asenjoinstacron:HNAAAspahttps://cmhnaaa.org.pe/ojs/index.php/rcmhnaaa/article/view/2762/1155Derechos de autor 2026 Javier Enciso-Benavides, Marlon Garcia-Paita, Santiago Alvarez-Vega, Salyoc Tapia-Rojas, Ana Mayanga-Herrera, Maria J. Ponshttps://creativecommons.org/licenses/by/4.0info:eu-repo/semantics/openAccessoai:cmhnaaa_ojs_cmhnaaa.cmhnaaa.org.pe:article/27622025-12-23T11:14:04Z
dc.title.none.fl_str_mv Assessment of specific cell-mediated immunity to SARS-CoV-2: techniques for its measurement
Evaluación de la inmunidad celular específica frente al SARS-CoV-2: técnicas para su medición
title Assessment of specific cell-mediated immunity to SARS-CoV-2: techniques for its measurement
spellingShingle Assessment of specific cell-mediated immunity to SARS-CoV-2: techniques for its measurement
Enciso-Benavides, Javier
Inmunidad adaptativa
linfocitos T
SARS-CoV-2
respuesta inmunológica
Adaptive Immunity
T Cells
SARS-CoV-2
Immunological response
title_short Assessment of specific cell-mediated immunity to SARS-CoV-2: techniques for its measurement
title_full Assessment of specific cell-mediated immunity to SARS-CoV-2: techniques for its measurement
title_fullStr Assessment of specific cell-mediated immunity to SARS-CoV-2: techniques for its measurement
title_full_unstemmed Assessment of specific cell-mediated immunity to SARS-CoV-2: techniques for its measurement
title_sort Assessment of specific cell-mediated immunity to SARS-CoV-2: techniques for its measurement
dc.creator.none.fl_str_mv Enciso-Benavides, Javier
Garcia-Paita, Marlon
Alvarez-Vega, Santiago
Tapia-Rojas, Salyoc
Mayanga-Herrera, Ana
Pons, Maria J.
author Enciso-Benavides, Javier
author_facet Enciso-Benavides, Javier
Garcia-Paita, Marlon
Alvarez-Vega, Santiago
Tapia-Rojas, Salyoc
Mayanga-Herrera, Ana
Pons, Maria J.
author_role author
author2 Garcia-Paita, Marlon
Alvarez-Vega, Santiago
Tapia-Rojas, Salyoc
Mayanga-Herrera, Ana
Pons, Maria J.
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv Inmunidad adaptativa
linfocitos T
SARS-CoV-2
respuesta inmunológica
Adaptive Immunity
T Cells
SARS-CoV-2
Immunological response
topic Inmunidad adaptativa
linfocitos T
SARS-CoV-2
respuesta inmunológica
Adaptive Immunity
T Cells
SARS-CoV-2
Immunological response
description Understanding the immune response to infection or vaccination against SARS-CoV-2 has become a key objective for the understanding of the disease, its progression, and protection against future infections or possible new variants that may emerge of the virus. The aim of the present review is to focus on the relevance of the cellular immune response and to summarize the different methodologies described for the study of this memory T-cell-related response, either after exposure to the virus or vaccination against SARS-CoV-2. For this purpose, cell proliferation assays (CFSE, 3H-thymidine), cell typing methods (flow cytometry), immunological assays based on the measurement of cytokines (ELISA, ELISPOT, suspension multiplex) and analysis of genes related to cellular immunity by molecular techniques (RT-qPCR or qPCR and sequencing) have been included.
publishDate 2026
dc.date.none.fl_str_mv 2026-01-29
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
texto
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://cmhnaaa.org.pe/ojs/index.php/rcmhnaaa/article/view/2762
10.35434/rcmhnaaa.2025.184.2762
url https://cmhnaaa.org.pe/ojs/index.php/rcmhnaaa/article/view/2762
identifier_str_mv 10.35434/rcmhnaaa.2025.184.2762
dc.language.none.fl_str_mv spa
language spa
dc.relation.none.fl_str_mv https://cmhnaaa.org.pe/ojs/index.php/rcmhnaaa/article/view/2762/1155
dc.rights.none.fl_str_mv https://creativecommons.org/licenses/by/4.0
info:eu-repo/semantics/openAccess
rights_invalid_str_mv https://creativecommons.org/licenses/by/4.0
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Cuerpo Médico del Hospital Nacional Almanzor Aguinaga Asenjo
publisher.none.fl_str_mv Cuerpo Médico del Hospital Nacional Almanzor Aguinaga Asenjo
dc.source.none.fl_str_mv Revista del Cuerpo Médico Hospital Nacional Almanzor Aguinaga Asenjo; Vol. 18 No. 4 (2025): Early Publication
Revista del Cuerpo Médico Hospital Nacional Almanzor Aguinaga Asenjo; Vol. 18 Núm. 4 (2025): Publicación Anticipada
2227-4731
2225-5109
10.35434/rcmhnaaa.2025.184
reponame:Revista del Cuerpo Médico Hospital Nacional Almanzor Aguinaga Asenjo
instname:Cuerpo Médico Hospital Nacional Almanzor Aguinaga Asenjo
instacron:HNAAA
instname_str Cuerpo Médico Hospital Nacional Almanzor Aguinaga Asenjo
instacron_str HNAAA
institution HNAAA
reponame_str Revista del Cuerpo Médico Hospital Nacional Almanzor Aguinaga Asenjo
collection Revista del Cuerpo Médico Hospital Nacional Almanzor Aguinaga Asenjo
repository.name.fl_str_mv
repository.mail.fl_str_mv
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Assessment of specific cell-mediated immunity to SARS-CoV-2: techniques for its measurement
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