Cloning leishmania telomeres in yeast by complementation

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Leishmania telemores were cloned in yeast by complementation using a half arm of the yeast artificial chromosome vector pJS97. A single copy marker associated to the telomere (YT2) located 6 kb away from one end of a megabase size chromosome of 1116c8 was cloned by end rescue and DNA sequenced. The...

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Detalles Bibliográficos
Autor: Espinoza, José
Formato: artículo
Fecha de Publicación:2003
Institución:Instituto Peruano de Energía Nuclear
Repositorio:IPEN-Institucional
Lenguaje:inglés
OAI Identifier:oai:repositorio.ipen.gob.pe:20.500.13054/363
Enlace del recurso:https://hdl.handle.net/20.500.13054/363
Nivel de acceso:acceso abierto
Materia:Leishmania telemores
Parásitos
Enfermedad de leishmaniasis
Secuencia de cromosomas
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spelling Espinoza, JoséEspinoza, José2015-06-24T16:45:46Z2015-06-24T16:45:46Z2003-07Espinoza J. Cloning leishmania telomeres in yeast by complementation. Informe Científico Tecnológico. Volumen 2 (2002) p. 147-149.1684-1662https://hdl.handle.net/20.500.13054/363Leishmania telemores were cloned in yeast by complementation using a half arm of the yeast artificial chromosome vector pJS97. A single copy marker associated to the telomere (YT2) located 6 kb away from one end of a megabase size chromosome of 1116c8 was cloned by end rescue and DNA sequenced. The sequence of 1479 bp did not show a significant homology with any sequence stored in GenBank. Leishmania telomeres are amenable to be cloned and maintained in yeast, this approach will facilitate the analysis of telomere and subtelomere regions which are usually underrepresented in genomic libraries.application/pdfengLima (Perú)PEinfo:eu-repo/semantics/openAccessInstituto Peruano de Energía NuclearRepositorio Institucional del Instituto Peruano de Energía Nuclearreponame:IPEN-Institucionalinstname:Instituto Peruano de Energía Nuclearinstacron:IPENLeishmania telemoresParásitosEnfermedad de leishmaniasisSecuencia de cromosomasCloning leishmania telomeres in yeast by complementationinfo:eu-repo/semantics/articleORIGINAL_147-149_ Telomeres ESPINOZA _r_.pdf_147-149_ Telomeres ESPINOZA _r_.pdfapplication/pdf216918https://repositorio.ipen.gob.pe/bitstream/20.500.13054/363/1/_147-149_%20Telomeres%20ESPINOZA%20_r_.pdfe0aa1583ecb28f49a24675f26bdcce9aMD51LICENSElicense.txtlicense.txttext/plain; charset=utf-8103https://repositorio.ipen.gob.pe/bitstream/20.500.13054/363/2/license.txt7ecf7b0639239de61a3a1ede9c397fecMD52TEXT_147-149_ Telomeres ESPINOZA _r_.pdf.txt_147-149_ Telomeres ESPINOZA _r_.pdf.txtExtracted texttext/plain7890https://repositorio.ipen.gob.pe/bitstream/20.500.13054/363/3/_147-149_%20Telomeres%20ESPINOZA%20_r_.pdf.txtd91799d0d524771bd5d3a6f6c2cc2be3MD53THUMBNAIL_147-149_ Telomeres ESPINOZA _r_.pdf.jpg_147-149_ Telomeres ESPINOZA _r_.pdf.jpgGenerated Thumbnailimage/jpeg23310https://repositorio.ipen.gob.pe/bitstream/20.500.13054/363/4/_147-149_%20Telomeres%20ESPINOZA%20_r_.pdf.jpg3eca4469d89668fe0d189995ff7590e0MD5420.500.13054/363oai:repositorio.ipen.gob.pe:20.500.13054/3632022-02-07 18:06:31.15Repositorio Institucional del Instituto Peruano de Energía Nuclearrepositorio@ipen.gob.peU2UgRGlzdHJpYnV5ZSBiYWpvIHVuYSBMaWNlbmNpYSBDcmVhdGl2ZSBDb21tb25zIEF0cmlidWNpw7NuLU5vQ29tZXJjaWFsLVNpbkRlcml2YXIgNC4wIEludGVybmFjaW9uYWwuCg==
dc.title.es_PE.fl_str_mv Cloning leishmania telomeres in yeast by complementation
title Cloning leishmania telomeres in yeast by complementation
spellingShingle Cloning leishmania telomeres in yeast by complementation
Espinoza, José
Leishmania telemores
Parásitos
Enfermedad de leishmaniasis
Secuencia de cromosomas
title_short Cloning leishmania telomeres in yeast by complementation
title_full Cloning leishmania telomeres in yeast by complementation
title_fullStr Cloning leishmania telomeres in yeast by complementation
title_full_unstemmed Cloning leishmania telomeres in yeast by complementation
title_sort Cloning leishmania telomeres in yeast by complementation
dc.creator.none.fl_str_mv Espinoza, José
author Espinoza, José
author_facet Espinoza, José
author_role author
dc.contributor.author.fl_str_mv Espinoza, José
dc.subject.es_PE.fl_str_mv Leishmania telemores
Parásitos
Enfermedad de leishmaniasis
Secuencia de cromosomas
topic Leishmania telemores
Parásitos
Enfermedad de leishmaniasis
Secuencia de cromosomas
description Leishmania telemores were cloned in yeast by complementation using a half arm of the yeast artificial chromosome vector pJS97. A single copy marker associated to the telomere (YT2) located 6 kb away from one end of a megabase size chromosome of 1116c8 was cloned by end rescue and DNA sequenced. The sequence of 1479 bp did not show a significant homology with any sequence stored in GenBank. Leishmania telomeres are amenable to be cloned and maintained in yeast, this approach will facilitate the analysis of telomere and subtelomere regions which are usually underrepresented in genomic libraries.
publishDate 2003
dc.date.accessioned.none.fl_str_mv 2015-06-24T16:45:46Z
dc.date.available.none.fl_str_mv 2015-06-24T16:45:46Z
dc.date.issued.fl_str_mv 2003-07
dc.type.es_PE.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.citation.es_PE.fl_str_mv Espinoza J. Cloning leishmania telomeres in yeast by complementation. Informe Científico Tecnológico. Volumen 2 (2002) p. 147-149.
dc.identifier.issn.none.fl_str_mv 1684-1662
dc.identifier.uri.none.fl_str_mv https://hdl.handle.net/20.500.13054/363
identifier_str_mv Espinoza J. Cloning leishmania telomeres in yeast by complementation. Informe Científico Tecnológico. Volumen 2 (2002) p. 147-149.
1684-1662
url https://hdl.handle.net/20.500.13054/363
dc.language.iso.es_PE.fl_str_mv eng
language eng
dc.rights.es_PE.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.es_PE.fl_str_mv application/pdf
dc.publisher.es_PE.fl_str_mv Lima (Perú)
dc.publisher.country.es_PE.fl_str_mv PE
dc.source.es_PE.fl_str_mv Instituto Peruano de Energía Nuclear
Repositorio Institucional del Instituto Peruano de Energía Nuclear
dc.source.none.fl_str_mv reponame:IPEN-Institucional
instname:Instituto Peruano de Energía Nuclear
instacron:IPEN
instname_str Instituto Peruano de Energía Nuclear
instacron_str IPEN
institution IPEN
reponame_str IPEN-Institucional
collection IPEN-Institucional
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Cloning leishmania telomeres in yeast by complementation
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