Effect of extenders based on tris, tes and skim milk on cryopreservation of epididymalalpaca sperm [Efecto de dilutores en base a tris, tes y leche descremada en la criopreservaciÓn de espermatozoides obtenidos del epidÍdimo de alpaca]
Descripción del Articulo
Se agradece al Consejo Nacional de Ciencia y Tecnología (CONCYTEC) por el financiamiento del presente trabajo a través del Proyecto PROCYT N.°157-2006 «Desarrollo de un protocolo de criopreservación de semen de alpacas mediante el estudio de diferentes dilutores y agentes crioprotectores sobre la fu...
| Autores: | , , , , , , |
|---|---|
| Formato: | artículo |
| Fecha de Publicación: | 2009 |
| Institución: | Consejo Nacional de Ciencia Tecnología e Innovación |
| Repositorio: | CONCYTEC-Institucional |
| Lenguaje: | español |
| OAI Identifier: | oai:repositorio.concytec.gob.pe:20.500.12390/895 |
| Enlace del recurso: | https://hdl.handle.net/20.500.12390/895 |
| Nivel de acceso: | acceso abierto |
| Materia: | espermatozoides epididimarios dilutores criopreservación de semen alpaca https://purl.org/pe-repo/ocde/ford#1.06.07 |
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CONC |
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CONCYTEC-Institucional |
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4689 |
| dc.title.none.fl_str_mv |
Effect of extenders based on tris, tes and skim milk on cryopreservation of epididymalalpaca sperm [Efecto de dilutores en base a tris, tes y leche descremada en la criopreservaciÓn de espermatozoides obtenidos del epidÍdimo de alpaca] |
| title |
Effect of extenders based on tris, tes and skim milk on cryopreservation of epididymalalpaca sperm [Efecto de dilutores en base a tris, tes y leche descremada en la criopreservaciÓn de espermatozoides obtenidos del epidÍdimo de alpaca] |
| spellingShingle |
Effect of extenders based on tris, tes and skim milk on cryopreservation of epididymalalpaca sperm [Efecto de dilutores en base a tris, tes y leche descremada en la criopreservaciÓn de espermatozoides obtenidos del epidÍdimo de alpaca] Jorge Banda R. espermatozoides epididimarios dilutores criopreservación de semen alpaca https://purl.org/pe-repo/ocde/ford#1.06.07 |
| title_short |
Effect of extenders based on tris, tes and skim milk on cryopreservation of epididymalalpaca sperm [Efecto de dilutores en base a tris, tes y leche descremada en la criopreservaciÓn de espermatozoides obtenidos del epidÍdimo de alpaca] |
| title_full |
Effect of extenders based on tris, tes and skim milk on cryopreservation of epididymalalpaca sperm [Efecto de dilutores en base a tris, tes y leche descremada en la criopreservaciÓn de espermatozoides obtenidos del epidÍdimo de alpaca] |
| title_fullStr |
Effect of extenders based on tris, tes and skim milk on cryopreservation of epididymalalpaca sperm [Efecto de dilutores en base a tris, tes y leche descremada en la criopreservaciÓn de espermatozoides obtenidos del epidÍdimo de alpaca] |
| title_full_unstemmed |
Effect of extenders based on tris, tes and skim milk on cryopreservation of epididymalalpaca sperm [Efecto de dilutores en base a tris, tes y leche descremada en la criopreservaciÓn de espermatozoides obtenidos del epidÍdimo de alpaca] |
| title_sort |
Effect of extenders based on tris, tes and skim milk on cryopreservation of epididymalalpaca sperm [Efecto de dilutores en base a tris, tes y leche descremada en la criopreservaciÓn de espermatozoides obtenidos del epidÍdimo de alpaca] |
| author |
Jorge Banda R. |
| author_facet |
Jorge Banda R. Shirley Evangelista V. Luis Ruiz G. Rocío Sandoval M. Claudia Rodríguez Ll. Martha Valdivia C. Alexei Santiani A. |
| author_role |
author |
| author2 |
Shirley Evangelista V. Luis Ruiz G. Rocío Sandoval M. Claudia Rodríguez Ll. Martha Valdivia C. Alexei Santiani A. |
| author2_role |
author author author author author author |
| dc.contributor.author.fl_str_mv |
Jorge Banda R. Shirley Evangelista V. Luis Ruiz G. Rocío Sandoval M. Claudia Rodríguez Ll. Martha Valdivia C. Alexei Santiani A. |
| dc.subject.none.fl_str_mv |
espermatozoides |
| topic |
espermatozoides epididimarios dilutores criopreservación de semen alpaca https://purl.org/pe-repo/ocde/ford#1.06.07 |
| dc.subject.es_PE.fl_str_mv |
epididimarios dilutores criopreservación de semen alpaca |
| dc.subject.ocde.none.fl_str_mv |
https://purl.org/pe-repo/ocde/ford#1.06.07 |
| description |
Se agradece al Consejo Nacional de Ciencia y Tecnología (CONCYTEC) por el financiamiento del presente trabajo a través del Proyecto PROCYT N.°157-2006 «Desarrollo de un protocolo de criopreservación de semen de alpacas mediante el estudio de diferentes dilutores y agentes crioprotectores sobre la funcionalidad espermática». |
| publishDate |
2009 |
| dc.date.accessioned.none.fl_str_mv |
2024-05-30T23:13:38Z |
| dc.date.available.none.fl_str_mv |
2024-05-30T23:13:38Z |
| dc.date.issued.fl_str_mv |
2009 |
| dc.type.none.fl_str_mv |
info:eu-repo/semantics/article |
| format |
article |
| dc.identifier.uri.none.fl_str_mv |
https://hdl.handle.net/20.500.12390/895 |
| dc.identifier.scopus.none.fl_str_mv |
2-s2.0-85047112658 |
| url |
https://hdl.handle.net/20.500.12390/895 |
| identifier_str_mv |
2-s2.0-85047112658 |
| dc.language.iso.none.fl_str_mv |
spa |
| language |
spa |
| dc.relation.ispartof.none.fl_str_mv |
Revista de Investigaciones Veterinarias del Peru |
| dc.rights.none.fl_str_mv |
info:eu-repo/semantics/openAccess |
| dc.rights.uri.none.fl_str_mv |
https://creativecommons.org/licenses/by-nc-nd/4.0/ |
| eu_rights_str_mv |
openAccess |
| rights_invalid_str_mv |
https://creativecommons.org/licenses/by-nc-nd/4.0/ |
| dc.publisher.none.fl_str_mv |
Universidad Nacional Mayor de San Marcos |
| publisher.none.fl_str_mv |
Universidad Nacional Mayor de San Marcos |
| dc.source.none.fl_str_mv |
reponame:CONCYTEC-Institucional instname:Consejo Nacional de Ciencia Tecnología e Innovación instacron:CONCYTEC |
| instname_str |
Consejo Nacional de Ciencia Tecnología e Innovación |
| instacron_str |
CONCYTEC |
| institution |
CONCYTEC |
| reponame_str |
CONCYTEC-Institucional |
| collection |
CONCYTEC-Institucional |
| repository.name.fl_str_mv |
Repositorio Institucional CONCYTEC |
| repository.mail.fl_str_mv |
repositorio@concytec.gob.pe |
| _version_ |
1844883001215811584 |
| spelling |
Publicationrp02359600rp02360600rp02363600rp02361600rp02357600rp02358600rp02362600Jorge Banda R.Shirley Evangelista V.Luis Ruiz G.Rocío Sandoval M.Claudia Rodríguez Ll.Martha Valdivia C.Alexei Santiani A.2024-05-30T23:13:38Z2024-05-30T23:13:38Z2009https://hdl.handle.net/20.500.12390/8952-s2.0-85047112658Se agradece al Consejo Nacional de Ciencia y Tecnología (CONCYTEC) por el financiamiento del presente trabajo a través del Proyecto PROCYT N.°157-2006 «Desarrollo de un protocolo de criopreservación de semen de alpacas mediante el estudio de diferentes dilutores y agentes crioprotectores sobre la funcionalidad espermática».The objective of this study was to evaluate the effect of three semen extenders (skim milk, Tris, and Tes) on cryopreservation of epididymal alpaca sperm. Previously, the effect of timespan since slaughtering or castration to the recovery of epididymal sperm was evaluated. Twenty-four alpaca testicles were used to obtain sperm from the epididymis in a buffered saline solution (PBS). The recovery of spermatozoa was performed at 0, 35, 48, and 72 hours (6 testes per group) after slaughtering or castration. Sperm motility, concentration, and sperm membrane functional integrity were analyzed. Sperm recovered after 35 hours was not usable for cryopreservation. Sperm samples recovered at 0 hours were subjected to the process of freezing. Samples were diluted with skim milk, Tris, and Tes, cooled from 35 to 5°C in 90 minutes, packed into 0.25 ml straws and frozen in liquid nitrogen. After thawing, straws were evaluated by motility, sperm membrane functional integrity and vitality/acrosome integrity. Motility was 17.0, 14.0, and 8.6% on skim milk, Tris and Tes groups respectively, where the skim milk group was significantly better than the Tes (p<0.05). Percentages of sperm membrane functional integrity and vitality/acrosomal integrity were similar among the three extenders. It was concluded that all three extenders provided similar effects for the cryopreservation of epididymal alpaca spermatozoa.Consejo Nacional de Ciencia, Tecnología e Innovación Tecnológica - ConcytecspaUniversidad Nacional Mayor de San MarcosRevista de Investigaciones Veterinarias del Peruinfo:eu-repo/semantics/openAccesshttps://creativecommons.org/licenses/by-nc-nd/4.0/espermatozoidesepididimarios-1dilutores-1criopreservación de semen-1alpaca-1https://purl.org/pe-repo/ocde/ford#1.06.07-1Effect of extenders based on tris, tes and skim milk on cryopreservation of epididymalalpaca sperm [Efecto de dilutores en base a tris, tes y leche descremada en la criopreservaciÓn de espermatozoides obtenidos del epidÍdimo de alpaca]info:eu-repo/semantics/articlereponame:CONCYTEC-Institucionalinstname:Consejo Nacional de Ciencia Tecnología e Innovacióninstacron:CONCYTEC20.500.12390/895oai:repositorio.concytec.gob.pe:20.500.12390/8952024-05-30 15:59:41.135https://creativecommons.org/licenses/by-nc-nd/4.0/info:eu-repo/semantics/openAccesshttp://purl.org/coar/access_right/c_14cbinfo:eu-repo/semantics/closedAccessmetadata only accesshttps://repositorio.concytec.gob.peRepositorio Institucional CONCYTECrepositorio@concytec.gob.pe#PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE#<Publication xmlns="https://www.openaire.eu/cerif-profile/1.1/" id="92106850-09f2-4faf-994f-42f6ef389d66"> <Type xmlns="https://www.openaire.eu/cerif-profile/vocab/COAR_Publication_Types">http://purl.org/coar/resource_type/c_1843</Type> <Language>spa</Language> <Title>Effect of extenders based on tris, tes and skim milk on cryopreservation of epididymalalpaca sperm [Efecto de dilutores en base a tris, tes y leche descremada en la criopreservaciÓn de espermatozoides obtenidos del epidÍdimo de alpaca]</Title> <PublishedIn> <Publication> <Title>Revista de Investigaciones Veterinarias del Peru</Title> </Publication> </PublishedIn> <PublicationDate>2009</PublicationDate> <SCP-Number>2-s2.0-85047112658</SCP-Number> <Authors> <Author> <DisplayName>Jorge Banda R.</DisplayName> <Person id="rp02359" /> <Affiliation> <OrgUnit> </OrgUnit> </Affiliation> </Author> <Author> <DisplayName>Shirley Evangelista V.</DisplayName> <Person id="rp02360" /> <Affiliation> <OrgUnit> </OrgUnit> </Affiliation> </Author> <Author> <DisplayName>Luis Ruiz G.</DisplayName> <Person id="rp02363" /> <Affiliation> <OrgUnit> </OrgUnit> </Affiliation> </Author> <Author> <DisplayName>Rocío Sandoval M.</DisplayName> <Person id="rp02361" /> <Affiliation> <OrgUnit> </OrgUnit> </Affiliation> </Author> <Author> <DisplayName>Claudia Rodríguez Ll.</DisplayName> <Person id="rp02357" /> <Affiliation> <OrgUnit> </OrgUnit> </Affiliation> </Author> <Author> <DisplayName>Martha Valdivia C.</DisplayName> <Person id="rp02358" /> <Affiliation> <OrgUnit> </OrgUnit> </Affiliation> </Author> <Author> <DisplayName>Alexei Santiani A.</DisplayName> <Person id="rp02362" /> <Affiliation> <OrgUnit> </OrgUnit> </Affiliation> </Author> </Authors> <Editors> </Editors> <Publishers> <Publisher> <DisplayName>Universidad Nacional Mayor de San Marcos</DisplayName> <OrgUnit /> </Publisher> </Publishers> <License>https://creativecommons.org/licenses/by-nc-nd/4.0/</License> <Keyword>espermatozoides</Keyword> <Keyword>epididimarios</Keyword> <Keyword>dilutores</Keyword> <Keyword>criopreservación de semen</Keyword> <Keyword>alpaca</Keyword> <Abstract>The objective of this study was to evaluate the effect of three semen extenders (skim milk, Tris, and Tes) on cryopreservation of epididymal alpaca sperm. Previously, the effect of timespan since slaughtering or castration to the recovery of epididymal sperm was evaluated. Twenty-four alpaca testicles were used to obtain sperm from the epididymis in a buffered saline solution (PBS). The recovery of spermatozoa was performed at 0, 35, 48, and 72 hours (6 testes per group) after slaughtering or castration. Sperm motility, concentration, and sperm membrane functional integrity were analyzed. Sperm recovered after 35 hours was not usable for cryopreservation. Sperm samples recovered at 0 hours were subjected to the process of freezing. Samples were diluted with skim milk, Tris, and Tes, cooled from 35 to 5°C in 90 minutes, packed into 0.25 ml straws and frozen in liquid nitrogen. After thawing, straws were evaluated by motility, sperm membrane functional integrity and vitality/acrosome integrity. Motility was 17.0, 14.0, and 8.6% on skim milk, Tris and Tes groups respectively, where the skim milk group was significantly better than the Tes (p<0.05). Percentages of sperm membrane functional integrity and vitality/acrosomal integrity were similar among the three extenders. It was concluded that all three extenders provided similar effects for the cryopreservation of epididymal alpaca spermatozoa.</Abstract> <Access xmlns="http://purl.org/coar/access_right" > </Access> </Publication> -1 |
| score |
13.377221 |
Nota importante:
La información contenida en este registro es de entera responsabilidad de la institución que gestiona el repositorio institucional donde esta contenido este documento o set de datos. El CONCYTEC no se hace responsable por los contenidos (publicaciones y/o datos) accesibles a través del Repositorio Nacional Digital de Ciencia, Tecnología e Innovación de Acceso Abierto (ALICIA).
La información contenida en este registro es de entera responsabilidad de la institución que gestiona el repositorio institucional donde esta contenido este documento o set de datos. El CONCYTEC no se hace responsable por los contenidos (publicaciones y/o datos) accesibles a través del Repositorio Nacional Digital de Ciencia, Tecnología e Innovación de Acceso Abierto (ALICIA).
