Local regulation of immune genes in rainbow trout (Oncorhynchus mykiss) naturally infected with Flavobacterium psychrophilum
Descripción del Articulo
The authors want to thank Lucía González and Laura Fernández for technical assistance. Dr. Erin Bromage (University of Massachusetts Dartmouth, USA) is also acknowledged for supplying the anti-IgD and anti-CD3 antibodies used in this study. This work was supported by the European Research Council (E...
| Autores: | , , , , , |
|---|---|
| Formato: | artículo |
| Fecha de Publicación: | 2019 |
| Institución: | Consejo Nacional de Ciencia Tecnología e Innovación |
| Repositorio: | CONCYTEC-Institucional |
| Lenguaje: | inglés |
| OAI Identifier: | oai:repositorio.concytec.gob.pe:20.500.12390/677 |
| Enlace del recurso: | https://hdl.handle.net/20.500.12390/677 https://doi.org/10.1016/j.fsi.2018.11.027 |
| Nivel de acceso: | acceso abierto |
| Materia: | gamma interferon CD4 antigen complementary DNA DNA 16S elongation factor 1alpha https://purl.org/pe-repo/ocde/ford#4.01.08 |
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4689 |
| dc.title.none.fl_str_mv |
Local regulation of immune genes in rainbow trout (Oncorhynchus mykiss) naturally infected with Flavobacterium psychrophilum |
| title |
Local regulation of immune genes in rainbow trout (Oncorhynchus mykiss) naturally infected with Flavobacterium psychrophilum |
| spellingShingle |
Local regulation of immune genes in rainbow trout (Oncorhynchus mykiss) naturally infected with Flavobacterium psychrophilum Muñoz-Atienza E. gamma interferon CD4 antigen complementary DNA DNA 16S elongation factor 1alpha https://purl.org/pe-repo/ocde/ford#4.01.08 |
| title_short |
Local regulation of immune genes in rainbow trout (Oncorhynchus mykiss) naturally infected with Flavobacterium psychrophilum |
| title_full |
Local regulation of immune genes in rainbow trout (Oncorhynchus mykiss) naturally infected with Flavobacterium psychrophilum |
| title_fullStr |
Local regulation of immune genes in rainbow trout (Oncorhynchus mykiss) naturally infected with Flavobacterium psychrophilum |
| title_full_unstemmed |
Local regulation of immune genes in rainbow trout (Oncorhynchus mykiss) naturally infected with Flavobacterium psychrophilum |
| title_sort |
Local regulation of immune genes in rainbow trout (Oncorhynchus mykiss) naturally infected with Flavobacterium psychrophilum |
| author |
Muñoz-Atienza E. |
| author_facet |
Muñoz-Atienza E. Távara C. Díaz-Rosales P. Llanco L. Serrano-Martínez E. Tafalla C. |
| author_role |
author |
| author2 |
Távara C. Díaz-Rosales P. Llanco L. Serrano-Martínez E. Tafalla C. |
| author2_role |
author author author author author |
| dc.contributor.author.fl_str_mv |
Muñoz-Atienza E. Távara C. Díaz-Rosales P. Llanco L. Serrano-Martínez E. Tafalla C. |
| dc.subject.none.fl_str_mv |
gamma interferon |
| topic |
gamma interferon CD4 antigen complementary DNA DNA 16S elongation factor 1alpha https://purl.org/pe-repo/ocde/ford#4.01.08 |
| dc.subject.es_PE.fl_str_mv |
CD4 antigen complementary DNA DNA 16S elongation factor 1alpha |
| dc.subject.ocde.none.fl_str_mv |
https://purl.org/pe-repo/ocde/ford#4.01.08 |
| description |
The authors want to thank Lucía González and Laura Fernández for technical assistance. Dr. Erin Bromage (University of Massachusetts Dartmouth, USA) is also acknowledged for supplying the anti-IgD and anti-CD3 antibodies used in this study. This work was supported by the European Research Council (ERC Consolidator Grant 2016 725061 TEMUBLYM). Carlos Távara would like to acknowledge the financial support from the Master Program in Aquatic Animal Health of the UPCH subsidized by Cienciactiva of CONCYTEC (Management Agreement No. 230-2015- FONDECYT-DE-PROMOCION 2). Carlos Távara also would like to acknowledge Vitapro S.A. for the interest in this research and support in the development of this work. E. Muñoz-Atienza holds a Juan de la Cierva-Formación Postdoctoral Contract (FJCI-2015-24452) funded by the Spanish Ministry of Economy, Industry and Competitiveness. |
| publishDate |
2019 |
| dc.date.accessioned.none.fl_str_mv |
2024-05-30T23:13:38Z |
| dc.date.available.none.fl_str_mv |
2024-05-30T23:13:38Z |
| dc.date.issued.fl_str_mv |
2019 |
| dc.type.none.fl_str_mv |
info:eu-repo/semantics/article |
| format |
article |
| dc.identifier.uri.none.fl_str_mv |
https://hdl.handle.net/20.500.12390/677 |
| dc.identifier.doi.none.fl_str_mv |
https://doi.org/10.1016/j.fsi.2018.11.027 |
| dc.identifier.scopus.none.fl_str_mv |
2-s2.0-85056652191 |
| url |
https://hdl.handle.net/20.500.12390/677 https://doi.org/10.1016/j.fsi.2018.11.027 |
| identifier_str_mv |
2-s2.0-85056652191 |
| dc.language.iso.none.fl_str_mv |
eng |
| language |
eng |
| dc.relation.ispartof.none.fl_str_mv |
Fish and Shellfish Immunology |
| dc.rights.none.fl_str_mv |
info:eu-repo/semantics/openAccess |
| eu_rights_str_mv |
openAccess |
| dc.publisher.none.fl_str_mv |
Academic Press |
| publisher.none.fl_str_mv |
Academic Press |
| dc.source.none.fl_str_mv |
reponame:CONCYTEC-Institucional instname:Consejo Nacional de Ciencia Tecnología e Innovación instacron:CONCYTEC |
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Consejo Nacional de Ciencia Tecnología e Innovación |
| instacron_str |
CONCYTEC |
| institution |
CONCYTEC |
| reponame_str |
CONCYTEC-Institucional |
| collection |
CONCYTEC-Institucional |
| repository.name.fl_str_mv |
Repositorio Institucional CONCYTEC |
| repository.mail.fl_str_mv |
repositorio@concytec.gob.pe |
| _version_ |
1844883006848761856 |
| spelling |
Publicationrp01529600rp01525600rp01528600rp01526600rp01530600rp01527600Muñoz-Atienza E.Távara C.Díaz-Rosales P.Llanco L.Serrano-Martínez E.Tafalla C.2024-05-30T23:13:38Z2024-05-30T23:13:38Z2019https://hdl.handle.net/20.500.12390/677https://doi.org/10.1016/j.fsi.2018.11.0272-s2.0-85056652191The authors want to thank Lucía González and Laura Fernández for technical assistance. Dr. Erin Bromage (University of Massachusetts Dartmouth, USA) is also acknowledged for supplying the anti-IgD and anti-CD3 antibodies used in this study. This work was supported by the European Research Council (ERC Consolidator Grant 2016 725061 TEMUBLYM). Carlos Távara would like to acknowledge the financial support from the Master Program in Aquatic Animal Health of the UPCH subsidized by Cienciactiva of CONCYTEC (Management Agreement No. 230-2015- FONDECYT-DE-PROMOCION 2). Carlos Távara also would like to acknowledge Vitapro S.A. for the interest in this research and support in the development of this work. E. Muñoz-Atienza holds a Juan de la Cierva-Formación Postdoctoral Contract (FJCI-2015-24452) funded by the Spanish Ministry of Economy, Industry and Competitiveness.Flavobacterium psychrophilum is the etiological agent of bacterial cold water disease (BCWD), also referred to as rainbow trout fry syndrome (RTFS), a disease with great economic impact in salmonid aquaculture. Despite this, to date, not many studies have analyzed in depth how the immune system is regulated during the course of the disease. In the current study, we have studied the transcription of several immune genes related to T and B cell activity in the skin of rainbow trout (Oncorhynchus mykiss) naturally infected with F. psychrophilum in a farm located in Lake Titicaca (Peru). The levels of expression of these genes were tested and compared to those obtained in asymptomatic and apparently healthy rainbow trout. In the case of symptomatic fish, skin samples containing characteristic ulcerative lesions were taken, as well as skin samples with no lesions. Our results pointed to a significant local up-regulation of IgD, CD4, CD8, perforin and IFNγ within the ulcerative lesions. On the other hand, no differences between the levels of expression of these genes were visible in the spleen. To confirm these results, the distribution of IgD+ and CD3+ cells was studied through immunohistochemical techniques in the ulcerative lesions. Our results demonstrate a strong local response to F. psychrophilum in rainbow trout in which IgD and T cells seem to play a major role.Consejo Nacional de Ciencia, Tecnología e Innovación Tecnológica - ConcytecengAcademic PressFish and Shellfish Immunologyinfo:eu-repo/semantics/openAccessgamma interferonCD4 antigen-1complementary DNA-1DNA 16S-1elongation factor 1alpha-1https://purl.org/pe-repo/ocde/ford#4.01.08-1Local regulation of immune genes in rainbow trout (Oncorhynchus mykiss) naturally infected with Flavobacterium psychrophiluminfo:eu-repo/semantics/articlereponame:CONCYTEC-Institucionalinstname:Consejo Nacional de Ciencia Tecnología e Innovacióninstacron:CONCYTEC#PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE#20.500.12390/677oai:repositorio.concytec.gob.pe:20.500.12390/6772024-05-30 15:35:58.661http://purl.org/coar/access_right/c_14cbinfo:eu-repo/semantics/closedAccessmetadata only accesshttps://repositorio.concytec.gob.peRepositorio Institucional CONCYTECrepositorio@concytec.gob.pe#PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE#<Publication xmlns="https://www.openaire.eu/cerif-profile/1.1/" id="9e6e5590-91b5-4329-b7b9-cdf8c200de82"> <Type xmlns="https://www.openaire.eu/cerif-profile/vocab/COAR_Publication_Types">http://purl.org/coar/resource_type/c_1843</Type> <Language>eng</Language> <Title>Local regulation of immune genes in rainbow trout (Oncorhynchus mykiss) naturally infected with Flavobacterium psychrophilum</Title> <PublishedIn> <Publication> <Title>Fish and Shellfish Immunology</Title> </Publication> </PublishedIn> <PublicationDate>2019</PublicationDate> <DOI>https://doi.org/10.1016/j.fsi.2018.11.027</DOI> <SCP-Number>2-s2.0-85056652191</SCP-Number> <Authors> <Author> <DisplayName>Muñoz-Atienza E.</DisplayName> <Person id="rp01529" /> <Affiliation> <OrgUnit> </OrgUnit> </Affiliation> </Author> <Author> <DisplayName>Távara C.</DisplayName> <Person id="rp01525" /> <Affiliation> <OrgUnit> </OrgUnit> </Affiliation> </Author> <Author> <DisplayName>Díaz-Rosales P.</DisplayName> <Person id="rp01528" /> <Affiliation> <OrgUnit> </OrgUnit> </Affiliation> </Author> <Author> <DisplayName>Llanco L.</DisplayName> <Person id="rp01526" /> <Affiliation> <OrgUnit> </OrgUnit> </Affiliation> </Author> <Author> <DisplayName>Serrano-Martínez E.</DisplayName> <Person id="rp01530" /> <Affiliation> <OrgUnit> </OrgUnit> </Affiliation> </Author> <Author> <DisplayName>Tafalla C.</DisplayName> <Person id="rp01527" /> <Affiliation> <OrgUnit> </OrgUnit> </Affiliation> </Author> </Authors> <Editors> </Editors> <Publishers> <Publisher> <DisplayName>Academic Press</DisplayName> <OrgUnit /> </Publisher> </Publishers> <Keyword>gamma interferon</Keyword> <Keyword>CD4 antigen</Keyword> <Keyword>complementary DNA</Keyword> <Keyword>DNA 16S</Keyword> <Keyword>elongation factor 1alpha</Keyword> <Abstract>Flavobacterium psychrophilum is the etiological agent of bacterial cold water disease (BCWD), also referred to as rainbow trout fry syndrome (RTFS), a disease with great economic impact in salmonid aquaculture. Despite this, to date, not many studies have analyzed in depth how the immune system is regulated during the course of the disease. In the current study, we have studied the transcription of several immune genes related to T and B cell activity in the skin of rainbow trout (Oncorhynchus mykiss) naturally infected with F. psychrophilum in a farm located in Lake Titicaca (Peru). The levels of expression of these genes were tested and compared to those obtained in asymptomatic and apparently healthy rainbow trout. In the case of symptomatic fish, skin samples containing characteristic ulcerative lesions were taken, as well as skin samples with no lesions. Our results pointed to a significant local up-regulation of IgD, CD4, CD8, perforin and IFNγ within the ulcerative lesions. On the other hand, no differences between the levels of expression of these genes were visible in the spleen. To confirm these results, the distribution of IgD+ and CD3+ cells was studied through immunohistochemical techniques in the ulcerative lesions. Our results demonstrate a strong local response to F. psychrophilum in rainbow trout in which IgD and T cells seem to play a major role.</Abstract> <Access xmlns="http://purl.org/coar/access_right" > </Access> </Publication> -1 |
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13.43463 |
Nota importante:
La información contenida en este registro es de entera responsabilidad de la institución que gestiona el repositorio institucional donde esta contenido este documento o set de datos. El CONCYTEC no se hace responsable por los contenidos (publicaciones y/o datos) accesibles a través del Repositorio Nacional Digital de Ciencia, Tecnología e Innovación de Acceso Abierto (ALICIA).
La información contenida en este registro es de entera responsabilidad de la institución que gestiona el repositorio institucional donde esta contenido este documento o set de datos. El CONCYTEC no se hace responsable por los contenidos (publicaciones y/o datos) accesibles a través del Repositorio Nacional Digital de Ciencia, Tecnología e Innovación de Acceso Abierto (ALICIA).
