Identification and heterologous expression of the kocurin biosynthetic gene cluster

Descripción del Articulo

The antibiotically bioactive thiopeptide compound kocurin was identified in extracts from a newly isolated Kocuria rosea strain. The axenic strain was retrieved from a soil sample of the intertidal area at the Paracas National Park, Peru. The genetic basis of this promising natural product with acti...

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Detalles Bibliográficos
Autores: Linares-Otoya, L, Linares-Otoya, V, Armas-Mantilla, L, Blanco-Olano, C, Crusemann, M, Ganoza-Yupanqui, ML, Campos-Florian, J, Konig, GM, Schaberle, TF
Formato: artículo
Fecha de Publicación:2017
Institución:Consejo Nacional de Ciencia Tecnología e Innovación
Repositorio:CONCYTEC-Institucional
Lenguaje:inglés
OAI Identifier:oai:repositorio.concytec.gob.pe:20.500.12390/1082
Enlace del recurso:https://hdl.handle.net/20.500.12390/1082
https://doi.org/10.1099/mic.0.000538
Nivel de acceso:acceso abierto
Materia:Genética
https://purl.org/pe-repo/ocde/ford#1.06.07
id CONC_c4b81e2d5f182cae62769330841a73fd
oai_identifier_str oai:repositorio.concytec.gob.pe:20.500.12390/1082
network_acronym_str CONC
network_name_str CONCYTEC-Institucional
repository_id_str 4689
dc.title.none.fl_str_mv Identification and heterologous expression of the kocurin biosynthetic gene cluster
title Identification and heterologous expression of the kocurin biosynthetic gene cluster
spellingShingle Identification and heterologous expression of the kocurin biosynthetic gene cluster
Linares-Otoya, L
Genética
https://purl.org/pe-repo/ocde/ford#1.06.07
title_short Identification and heterologous expression of the kocurin biosynthetic gene cluster
title_full Identification and heterologous expression of the kocurin biosynthetic gene cluster
title_fullStr Identification and heterologous expression of the kocurin biosynthetic gene cluster
title_full_unstemmed Identification and heterologous expression of the kocurin biosynthetic gene cluster
title_sort Identification and heterologous expression of the kocurin biosynthetic gene cluster
author Linares-Otoya, L
author_facet Linares-Otoya, L
Linares-Otoya, V
Armas-Mantilla, L
Blanco-Olano, C
Crusemann, M
Ganoza-Yupanqui, ML
Campos-Florian, J
Konig, GM
Schaberle, TF
author_role author
author2 Linares-Otoya, V
Armas-Mantilla, L
Blanco-Olano, C
Crusemann, M
Ganoza-Yupanqui, ML
Campos-Florian, J
Konig, GM
Schaberle, TF
author2_role author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Linares-Otoya, L
Linares-Otoya, V
Armas-Mantilla, L
Blanco-Olano, C
Crusemann, M
Ganoza-Yupanqui, ML
Campos-Florian, J
Konig, GM
Schaberle, TF
dc.subject.none.fl_str_mv Genética
topic Genética
https://purl.org/pe-repo/ocde/ford#1.06.07
dc.subject.ocde.none.fl_str_mv https://purl.org/pe-repo/ocde/ford#1.06.07
description The antibiotically bioactive thiopeptide compound kocurin was identified in extracts from a newly isolated Kocuria rosea strain. The axenic strain was retrieved from a soil sample of the intertidal area at the Paracas National Park, Peru. The genetic basis of this promising natural product with activity against methicillin-resistant Staphylococcus aureus (MRSA) strains was revealed by comparative genome analysis of this new isolate and other reported thiopeptide producer strains. The functionality of the predicted gene locus was experimentally proven by heterologous expression in Streptomyces coelicolor M1146. Expression of the gene cluster under the control of a constitutive promoter enabled the transgenic strain to produce kocurin in selected media. The kocurin biosynthetic gene cluster comprises nine open reading frames and spans around 12 kbp of the genome.
publishDate 2017
dc.date.accessioned.none.fl_str_mv 2024-05-30T23:13:38Z
dc.date.available.none.fl_str_mv 2024-05-30T23:13:38Z
dc.date.issued.fl_str_mv 2017
dc.type.none.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.uri.none.fl_str_mv https://hdl.handle.net/20.500.12390/1082
dc.identifier.doi.none.fl_str_mv https://doi.org/10.1099/mic.0.000538
dc.identifier.isi.none.fl_str_mv 413495300005
url https://hdl.handle.net/20.500.12390/1082
https://doi.org/10.1099/mic.0.000538
identifier_str_mv 413495300005
dc.language.iso.none.fl_str_mv eng
language eng
dc.relation.ispartof.none.fl_str_mv Microbiology-SGM
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Microbiology Society
publisher.none.fl_str_mv Microbiology Society
dc.source.none.fl_str_mv reponame:CONCYTEC-Institucional
instname:Consejo Nacional de Ciencia Tecnología e Innovación
instacron:CONCYTEC
instname_str Consejo Nacional de Ciencia Tecnología e Innovación
instacron_str CONCYTEC
institution CONCYTEC
reponame_str CONCYTEC-Institucional
collection CONCYTEC-Institucional
repository.name.fl_str_mv Repositorio Institucional CONCYTEC
repository.mail.fl_str_mv repositorio@concytec.gob.pe
_version_ 1844883027631538176
spelling Publicationrp00933500rp00927500rp00934500rp01066500rp00928500rp00932500rp00931500rp00929500rp00935500Linares-Otoya, LLinares-Otoya, VArmas-Mantilla, LBlanco-Olano, CCrusemann, MGanoza-Yupanqui, MLCampos-Florian, JKonig, GMSchaberle, TF2024-05-30T23:13:38Z2024-05-30T23:13:38Z2017https://hdl.handle.net/20.500.12390/1082https://doi.org/10.1099/mic.0.000538413495300005The antibiotically bioactive thiopeptide compound kocurin was identified in extracts from a newly isolated Kocuria rosea strain. The axenic strain was retrieved from a soil sample of the intertidal area at the Paracas National Park, Peru. The genetic basis of this promising natural product with activity against methicillin-resistant Staphylococcus aureus (MRSA) strains was revealed by comparative genome analysis of this new isolate and other reported thiopeptide producer strains. The functionality of the predicted gene locus was experimentally proven by heterologous expression in Streptomyces coelicolor M1146. Expression of the gene cluster under the control of a constitutive promoter enabled the transgenic strain to produce kocurin in selected media. The kocurin biosynthetic gene cluster comprises nine open reading frames and spans around 12 kbp of the genome.Consejo Nacional de Ciencia, Tecnología e Innovación Tecnológica - ConcytecengMicrobiology SocietyMicrobiology-SGMinfo:eu-repo/semantics/openAccessGenéticahttps://purl.org/pe-repo/ocde/ford#1.06.07-1Identification and heterologous expression of the kocurin biosynthetic gene clusterinfo:eu-repo/semantics/articlereponame:CONCYTEC-Institucionalinstname:Consejo Nacional de Ciencia Tecnología e Innovacióninstacron:CONCYTEC20.500.12390/1082oai:repositorio.concytec.gob.pe:20.500.12390/10822024-05-30 16:01:03.676http://purl.org/coar/access_right/c_14cbinfo:eu-repo/semantics/closedAccessmetadata only accesshttps://repositorio.concytec.gob.peRepositorio Institucional CONCYTECrepositorio@concytec.gob.pe#PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE#<Publication xmlns="https://www.openaire.eu/cerif-profile/1.1/" id="c4e19c08-53e3-4dde-9aa1-20c86301351d"> <Type xmlns="https://www.openaire.eu/cerif-profile/vocab/COAR_Publication_Types">http://purl.org/coar/resource_type/c_1843</Type> <Language>eng</Language> <Title>Identification and heterologous expression of the kocurin biosynthetic gene cluster</Title> <PublishedIn> <Publication> <Title>Microbiology-SGM</Title> </Publication> </PublishedIn> <PublicationDate>2017</PublicationDate> <DOI>https://doi.org/10.1099/mic.0.000538</DOI> <ISI-Number>413495300005</ISI-Number> <Authors> <Author> <DisplayName>Linares-Otoya, L</DisplayName> <Person id="rp00933" /> <Affiliation> <OrgUnit> </OrgUnit> </Affiliation> </Author> <Author> <DisplayName>Linares-Otoya, V</DisplayName> <Person id="rp00927" /> <Affiliation> <OrgUnit> </OrgUnit> </Affiliation> </Author> <Author> <DisplayName>Armas-Mantilla, L</DisplayName> <Person id="rp00934" /> <Affiliation> <OrgUnit> </OrgUnit> </Affiliation> </Author> <Author> <DisplayName>Blanco-Olano, C</DisplayName> <Person id="rp01066" /> <Affiliation> <OrgUnit> </OrgUnit> </Affiliation> </Author> <Author> <DisplayName>Crusemann, M</DisplayName> <Person id="rp00928" /> <Affiliation> <OrgUnit> </OrgUnit> </Affiliation> </Author> <Author> <DisplayName>Ganoza-Yupanqui, ML</DisplayName> <Person id="rp00932" /> <Affiliation> <OrgUnit> </OrgUnit> </Affiliation> </Author> <Author> <DisplayName>Campos-Florian, J</DisplayName> <Person id="rp00931" /> <Affiliation> <OrgUnit> </OrgUnit> </Affiliation> </Author> <Author> <DisplayName>Konig, GM</DisplayName> <Person id="rp00929" /> <Affiliation> <OrgUnit> </OrgUnit> </Affiliation> </Author> <Author> <DisplayName>Schaberle, TF</DisplayName> <Person id="rp00935" /> <Affiliation> <OrgUnit> </OrgUnit> </Affiliation> </Author> </Authors> <Editors> </Editors> <Publishers> <Publisher> <DisplayName>Microbiology Society</DisplayName> <OrgUnit /> </Publisher> </Publishers> <Keyword>Genética</Keyword> <Abstract>The antibiotically bioactive thiopeptide compound kocurin was identified in extracts from a newly isolated Kocuria rosea strain. The axenic strain was retrieved from a soil sample of the intertidal area at the Paracas National Park, Peru. The genetic basis of this promising natural product with activity against methicillin-resistant Staphylococcus aureus (MRSA) strains was revealed by comparative genome analysis of this new isolate and other reported thiopeptide producer strains. The functionality of the predicted gene locus was experimentally proven by heterologous expression in Streptomyces coelicolor M1146. Expression of the gene cluster under the control of a constitutive promoter enabled the transgenic strain to produce kocurin in selected media. The kocurin biosynthetic gene cluster comprises nine open reading frames and spans around 12 kbp of the genome.</Abstract> <Access xmlns="http://purl.org/coar/access_right" > </Access> </Publication> -1
score 13.881255
Identification and heterologous expression of the kocurin biosynthetic gene cluster
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